Proteins and antibodies in serum, plasma, and whole blood—size characterization using asymmetrical flow field-flow fractionation (AF4)
(2018) In Analytical and Bioanalytical Chemistry 410(20). p.4867-4873- Abstract
The analysis of aggregates of therapeutic proteins is crucial in order to ensure efficacy and patient safety. Typically, the analysis is performed in the finished formulation to ensure that aggregates are not present. An important question is, however, what happens to therapeutic proteins, with regard to oligomerization and aggregation, after they have been administrated (i.e., in the blood). In this paper, the separation of whole blood, plasma, and serum is shown using asymmetric flow field-flow fractionation (AF4) with a minimum of sample pre-treatment. Furthermore, the analysis and size characterization of a fluorescent antibody in blood plasma using AF4 are demonstrated. The results show the suitability and strength of AF4 for blood... (More)
The analysis of aggregates of therapeutic proteins is crucial in order to ensure efficacy and patient safety. Typically, the analysis is performed in the finished formulation to ensure that aggregates are not present. An important question is, however, what happens to therapeutic proteins, with regard to oligomerization and aggregation, after they have been administrated (i.e., in the blood). In this paper, the separation of whole blood, plasma, and serum is shown using asymmetric flow field-flow fractionation (AF4) with a minimum of sample pre-treatment. Furthermore, the analysis and size characterization of a fluorescent antibody in blood plasma using AF4 are demonstrated. The results show the suitability and strength of AF4 for blood analysis and open new important routes for the analysis and characterization of therapeutic proteins in the blood.
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- author
- Leeman, Mats LU ; Choi, Jaeyeong LU ; Hansson, Sebastian LU ; Storm, Matilda Ulmius and Nilsson, Lars LU
- organization
- publishing date
- 2018-05-29
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Antibodies, Asymmetric flow field-flow fractionation (AF4), Fluorescence labelling, Plasma, Serum, Whole blood
- in
- Analytical and Bioanalytical Chemistry
- volume
- 410
- issue
- 20
- pages
- 4867 - 4873
- publisher
- Springer
- external identifiers
-
- pmid:29808297
- scopus:85047660690
- ISSN
- 1618-2642
- DOI
- 10.1007/s00216-018-1127-2
- language
- English
- LU publication?
- yes
- id
- 56cf8c7a-35be-4273-bee6-ceb799ef3df9
- date added to LUP
- 2018-06-12 15:46:44
- date last changed
- 2024-09-17 22:18:42
@article{56cf8c7a-35be-4273-bee6-ceb799ef3df9, abstract = {{<p>The analysis of aggregates of therapeutic proteins is crucial in order to ensure efficacy and patient safety. Typically, the analysis is performed in the finished formulation to ensure that aggregates are not present. An important question is, however, what happens to therapeutic proteins, with regard to oligomerization and aggregation, after they have been administrated (i.e., in the blood). In this paper, the separation of whole blood, plasma, and serum is shown using asymmetric flow field-flow fractionation (AF4) with a minimum of sample pre-treatment. Furthermore, the analysis and size characterization of a fluorescent antibody in blood plasma using AF4 are demonstrated. The results show the suitability and strength of AF4 for blood analysis and open new important routes for the analysis and characterization of therapeutic proteins in the blood.</p>}}, author = {{Leeman, Mats and Choi, Jaeyeong and Hansson, Sebastian and Storm, Matilda Ulmius and Nilsson, Lars}}, issn = {{1618-2642}}, keywords = {{Antibodies; Asymmetric flow field-flow fractionation (AF4); Fluorescence labelling; Plasma; Serum; Whole blood}}, language = {{eng}}, month = {{05}}, number = {{20}}, pages = {{4867--4873}}, publisher = {{Springer}}, series = {{Analytical and Bioanalytical Chemistry}}, title = {{Proteins and antibodies in serum, plasma, and whole blood—size characterization using asymmetrical flow field-flow fractionation (AF4)}}, url = {{http://dx.doi.org/10.1007/s00216-018-1127-2}}, doi = {{10.1007/s00216-018-1127-2}}, volume = {{410}}, year = {{2018}}, }