High-fat diet consumption alters energy metabolism in the mouse hypothalamus
(2019) In International Journal of Obesity 43(6). p.1295-1304- Abstract
Background/Objectives: High-fat diet consumption is known to trigger an inflammatory response in the hypothalamus, which has been characterized by an initial expression of pro-inflammatory genes followed by hypothalamic astrocytosis, microgliosis, and the appearance of neuronal injury markers. The specific effects of high-fat diet on hypothalamic energy metabolism and neurotransmission are however not yet known and have not been investigated before. Subjects/Methods: We used 1H and 13C magnetic resonance spectroscopy (MRS) and immunofluorescence techniques to evaluate in vivo the consequences of high-saturated fat diet administration to mice, and explored the effects on hypothalamic metabolism in three mouse... (More)
Background/Objectives: High-fat diet consumption is known to trigger an inflammatory response in the hypothalamus, which has been characterized by an initial expression of pro-inflammatory genes followed by hypothalamic astrocytosis, microgliosis, and the appearance of neuronal injury markers. The specific effects of high-fat diet on hypothalamic energy metabolism and neurotransmission are however not yet known and have not been investigated before. Subjects/Methods: We used 1H and 13C magnetic resonance spectroscopy (MRS) and immunofluorescence techniques to evaluate in vivo the consequences of high-saturated fat diet administration to mice, and explored the effects on hypothalamic metabolism in three mouse cohorts at different time points for up to 4 months. Results: We found that high-fat diet increases significantly the hypothalamic levels of glucose (P < 0.001), osmolytes (P < 0.001), and neurotransmitters (P < 0.05) from 2 months of diet, and alters the rates of metabolic (P < 0.05) and neurotransmission fluxes (P < 0.001), and the contribution of non-glycolytic substrates to hypothalamic metabolism (P < 0.05) after 10 weeks of high-fat feeding. Conclusions/interpretation: We report changes that reveal a high-fat diet-induced alteration of hypothalamic metabolism and neurotransmission that is quantifiable by 1H and 13C MRS in vivo, and present the first evidence of the extension of the inflammation pathology to a localized metabolic imbalance.
(Less)
- author
- Lizarbe, Blanca ; Cherix, Antoine ; Duarte, João M.N. LU ; Cardinaux, Jean René and Gruetter, Rolf
- organization
- publishing date
- 2019
- type
- Contribution to journal
- publication status
- published
- subject
- in
- International Journal of Obesity
- volume
- 43
- issue
- 6
- pages
- 1295 - 1304
- publisher
- Nature Publishing Group
- external identifiers
-
- scopus:85054616210
- pmid:30301962
- ISSN
- 0307-0565
- DOI
- 10.1038/s41366-018-0224-9
- language
- English
- LU publication?
- yes
- id
- 76330b89-219c-43c5-8fb7-ba1669ed12ea
- date added to LUP
- 2018-11-13 10:01:09
- date last changed
- 2024-09-18 06:13:15
@article{76330b89-219c-43c5-8fb7-ba1669ed12ea, abstract = {{<p>Background/Objectives: High-fat diet consumption is known to trigger an inflammatory response in the hypothalamus, which has been characterized by an initial expression of pro-inflammatory genes followed by hypothalamic astrocytosis, microgliosis, and the appearance of neuronal injury markers. The specific effects of high-fat diet on hypothalamic energy metabolism and neurotransmission are however not yet known and have not been investigated before. Subjects/Methods: We used <sup>1</sup>H and <sup>13</sup>C magnetic resonance spectroscopy (MRS) and immunofluorescence techniques to evaluate in vivo the consequences of high-saturated fat diet administration to mice, and explored the effects on hypothalamic metabolism in three mouse cohorts at different time points for up to 4 months. Results: We found that high-fat diet increases significantly the hypothalamic levels of glucose (P < 0.001), osmolytes (P < 0.001), and neurotransmitters (P < 0.05) from 2 months of diet, and alters the rates of metabolic (P < 0.05) and neurotransmission fluxes (P < 0.001), and the contribution of non-glycolytic substrates to hypothalamic metabolism (P < 0.05) after 10 weeks of high-fat feeding. Conclusions/interpretation: We report changes that reveal a high-fat diet-induced alteration of hypothalamic metabolism and neurotransmission that is quantifiable by <sup>1</sup>H and <sup>13</sup>C MRS in vivo, and present the first evidence of the extension of the inflammation pathology to a localized metabolic imbalance.</p>}}, author = {{Lizarbe, Blanca and Cherix, Antoine and Duarte, João M.N. and Cardinaux, Jean René and Gruetter, Rolf}}, issn = {{0307-0565}}, language = {{eng}}, number = {{6}}, pages = {{1295--1304}}, publisher = {{Nature Publishing Group}}, series = {{International Journal of Obesity}}, title = {{High-fat diet consumption alters energy metabolism in the mouse hypothalamus}}, url = {{http://dx.doi.org/10.1038/s41366-018-0224-9}}, doi = {{10.1038/s41366-018-0224-9}}, volume = {{43}}, year = {{2019}}, }