Comprehensive analysis of microRNA expression in regionalized human neural progenitor cells reveals microRNA-10 as a caudalizing factor.
(2015) In Development: For advances in developmental biology and stem cells 142(18). p.3166-3177- Abstract
- MicroRNAs (miRNAs) have been implicated in regulating multiple processes during brain development in various species. However, the function of miRNAs in human brain development remains largely unexplored. Here, we provide a comprehensive analysis of miRNA expression of regionalized neural progenitor cells derived from human embryonic stem cells and human foetal brain. We found miR-92b-3p and miR-130b-5p to be specifically associated with neural progenitors and several miRNAs that display both age-specific and region-specific expression patterns. Among these miRNAs, we identified miR-10 to be specifically expressed in the human hindbrain and spinal cord, while being absent from rostral regions. We found that miR-10 regulates a large number... (More)
- MicroRNAs (miRNAs) have been implicated in regulating multiple processes during brain development in various species. However, the function of miRNAs in human brain development remains largely unexplored. Here, we provide a comprehensive analysis of miRNA expression of regionalized neural progenitor cells derived from human embryonic stem cells and human foetal brain. We found miR-92b-3p and miR-130b-5p to be specifically associated with neural progenitors and several miRNAs that display both age-specific and region-specific expression patterns. Among these miRNAs, we identified miR-10 to be specifically expressed in the human hindbrain and spinal cord, while being absent from rostral regions. We found that miR-10 regulates a large number of genes enriched for functions including transcription, actin cytoskeleton and ephrin receptor signalling. When overexpressed, miR-10 influences caudalization of human neural progenitor cells. Together, these data confirm a role for miRNAs in establishing different human neural progenitor populations. This dataset also provides a comprehensive resource for future studies investigating the functional role of different miRNAs in human brain development. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/8035305
- author
- Jönsson, Marie LU ; Nelander Wahlestedt, Jenny LU ; Åkerblom, Malin LU ; Kirkeby, Agnete LU ; Malmevik, Josephine LU ; Brattaas, Per Ludvik LU ; Jakobsson, Johan LU and Parmar, Malin LU
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Development: For advances in developmental biology and stem cells
- volume
- 142
- issue
- 18
- pages
- 3166 - 3177
- publisher
- The Company of Biologists Ltd
- external identifiers
-
- pmid:26395143
- wos:000361742600011
- scopus:84942163494
- pmid:26395143
- ISSN
- 1477-9129
- DOI
- 10.1242/dev.122747
- language
- English
- LU publication?
- yes
- id
- 27f1747e-77b4-4223-a7bf-5e303ef38341 (old id 8035305)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/26395143?dopt=Abstract
- date added to LUP
- 2016-04-01 10:32:53
- date last changed
- 2022-05-13 18:02:30
@article{27f1747e-77b4-4223-a7bf-5e303ef38341, abstract = {{MicroRNAs (miRNAs) have been implicated in regulating multiple processes during brain development in various species. However, the function of miRNAs in human brain development remains largely unexplored. Here, we provide a comprehensive analysis of miRNA expression of regionalized neural progenitor cells derived from human embryonic stem cells and human foetal brain. We found miR-92b-3p and miR-130b-5p to be specifically associated with neural progenitors and several miRNAs that display both age-specific and region-specific expression patterns. Among these miRNAs, we identified miR-10 to be specifically expressed in the human hindbrain and spinal cord, while being absent from rostral regions. We found that miR-10 regulates a large number of genes enriched for functions including transcription, actin cytoskeleton and ephrin receptor signalling. When overexpressed, miR-10 influences caudalization of human neural progenitor cells. Together, these data confirm a role for miRNAs in establishing different human neural progenitor populations. This dataset also provides a comprehensive resource for future studies investigating the functional role of different miRNAs in human brain development.}}, author = {{Jönsson, Marie and Nelander Wahlestedt, Jenny and Åkerblom, Malin and Kirkeby, Agnete and Malmevik, Josephine and Brattaas, Per Ludvik and Jakobsson, Johan and Parmar, Malin}}, issn = {{1477-9129}}, language = {{eng}}, number = {{18}}, pages = {{3166--3177}}, publisher = {{The Company of Biologists Ltd}}, series = {{Development: For advances in developmental biology and stem cells}}, title = {{Comprehensive analysis of microRNA expression in regionalized human neural progenitor cells reveals microRNA-10 as a caudalizing factor.}}, url = {{http://dx.doi.org/10.1242/dev.122747}}, doi = {{10.1242/dev.122747}}, volume = {{142}}, year = {{2015}}, }