Label-free concentration of viable neurons, hESCs and cancer cells by means of acoustophoresis.
(2016) In Integrative Biology 8(3). p.332-340- Abstract
- Concentration of viable cell populations in suspension is of interest for several clinical and pre-clinical applications. Here, we report that microfluidic acoustophoresis is an effective method to efficiently concentrate live and viable cells with high target purity without any need for protein fluorescent labeling using antibodies or over-expression. We explored the effect of the acoustic field acoustic energy density and systematically used different protocols to induce apoptosis or cell death and then determined the efficiency of live and dead cell separation. We used the breast cancer cell line MCF-7, the mouse neuroblastoma N2a as well as human embryonic stem cells (hESCs) to demonstrate that this method is gentle and can be applied... (More)
- Concentration of viable cell populations in suspension is of interest for several clinical and pre-clinical applications. Here, we report that microfluidic acoustophoresis is an effective method to efficiently concentrate live and viable cells with high target purity without any need for protein fluorescent labeling using antibodies or over-expression. We explored the effect of the acoustic field acoustic energy density and systematically used different protocols to induce apoptosis or cell death and then determined the efficiency of live and dead cell separation. We used the breast cancer cell line MCF-7, the mouse neuroblastoma N2a as well as human embryonic stem cells (hESCs) to demonstrate that this method is gentle and can be applied to different cell populations. First, we induced cell death by means of high osmotic shock using a high concentration of PBS (10×), the protein kinase inhibitor staurosporine, high concentrations of dimethyl sulfoxide (DMSO, 10%), and finally, cell starvation. In all the methods employed, we successfully induced cell death and were able to purify and concentrate the remaining live cells using acoustophoresis. Importantly, the concentration of viable cells was not dependent on a specific cell type. Further, we demonstrate that different death inducing stimuli have different effects on the intrinsic cell properties and therefore affect the efficiency of the acoustophoretic separation. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/8821651
- author
- Castro Zalis, Marina LU ; Reyes, Juan F LU ; Augustsson, Per LU ; Holmqvist, Staffan LU ; Roybon, Laurent LU ; Laurell, Thomas LU and Deierborg, Tomas LU
- organization
-
- MultiPark: Multidisciplinary research focused on Parkinson´s disease
- NanoLund: Centre for Nanoscience
- Ophthalmology, Lund
- Department of Experimental Medical Science
- Department of Biomedical Engineering
- IPSC Laboratory for CNS Disease Modeling (research group)
- BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
- Neuroinflammation (research group)
- publishing date
- 2016-02-26
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Integrative Biology
- volume
- 8
- issue
- 3
- pages
- 9 pages
- publisher
- Royal Society of Chemistry
- external identifiers
-
- pmid:26915333
- scopus:84960961317
- wos:000372256400007
- pmid:26915333
- ISSN
- 1757-9708
- DOI
- 10.1039/c5ib00288e
- language
- English
- LU publication?
- yes
- id
- ac318363-3704-402e-8c0c-f4cdfc8e3ef8 (old id 8821651)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/26915333?dopt=Abstract
- date added to LUP
- 2016-04-01 10:45:03
- date last changed
- 2023-11-10 04:21:31
@article{ac318363-3704-402e-8c0c-f4cdfc8e3ef8, abstract = {{Concentration of viable cell populations in suspension is of interest for several clinical and pre-clinical applications. Here, we report that microfluidic acoustophoresis is an effective method to efficiently concentrate live and viable cells with high target purity without any need for protein fluorescent labeling using antibodies or over-expression. We explored the effect of the acoustic field acoustic energy density and systematically used different protocols to induce apoptosis or cell death and then determined the efficiency of live and dead cell separation. We used the breast cancer cell line MCF-7, the mouse neuroblastoma N2a as well as human embryonic stem cells (hESCs) to demonstrate that this method is gentle and can be applied to different cell populations. First, we induced cell death by means of high osmotic shock using a high concentration of PBS (10×), the protein kinase inhibitor staurosporine, high concentrations of dimethyl sulfoxide (DMSO, 10%), and finally, cell starvation. In all the methods employed, we successfully induced cell death and were able to purify and concentrate the remaining live cells using acoustophoresis. Importantly, the concentration of viable cells was not dependent on a specific cell type. Further, we demonstrate that different death inducing stimuli have different effects on the intrinsic cell properties and therefore affect the efficiency of the acoustophoretic separation.}}, author = {{Castro Zalis, Marina and Reyes, Juan F and Augustsson, Per and Holmqvist, Staffan and Roybon, Laurent and Laurell, Thomas and Deierborg, Tomas}}, issn = {{1757-9708}}, language = {{eng}}, month = {{02}}, number = {{3}}, pages = {{332--340}}, publisher = {{Royal Society of Chemistry}}, series = {{Integrative Biology}}, title = {{Label-free concentration of viable neurons, hESCs and cancer cells by means of acoustophoresis.}}, url = {{http://dx.doi.org/10.1039/c5ib00288e}}, doi = {{10.1039/c5ib00288e}}, volume = {{8}}, year = {{2016}}, }