Bacillus subtilis cytochrome oxidase mutants: biochemical analysis and genetic evidence for two aa3-type oxidases
(1991) In Molecular Microbiology 5(8). p.2063-2072- Abstract
- The ctaBCDEF genes coding for cytochrome c oxidase were found to reside adjacent to a regulatory gene ctaA at 127-degrees on the Bacillus subtilis chromosome. The structural genes for subunits I and II, ctaD and ctaC, were deleted by gene-replacement using a phleomycin-resistance marker. The mutant was unable to oxidize N,N,N',N'-tetramethyl-p-phenylenediamine and oxidized cytochrome c at a significantly lower rate. Absorption spectra of the mutant and wild-type membranes confirmed the presence of two haem A-containing enzymes in B. subtilis. Another mutant, with a spontaneous deletion upstream from ctaC, was found to express neither of these enzymes. Radioactive haem-labelling was used to identify subunit 11, which contains a haem C, and... (More)
- The ctaBCDEF genes coding for cytochrome c oxidase were found to reside adjacent to a regulatory gene ctaA at 127-degrees on the Bacillus subtilis chromosome. The structural genes for subunits I and II, ctaD and ctaC, were deleted by gene-replacement using a phleomycin-resistance marker. The mutant was unable to oxidize N,N,N',N'-tetramethyl-p-phenylenediamine and oxidized cytochrome c at a significantly lower rate. Absorption spectra of the mutant and wild-type membranes confirmed the presence of two haem A-containing enzymes in B. subtilis. Another mutant, with a spontaneous deletion upstream from ctaC, was found to express neither of these enzymes. Radioactive haem-labelling was used to identify subunit 11, which contains a haem C, and cytochrome c-550 among the membrane-bound c-type cytochromes of B. subtilis. (Less)
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https://lup.lub.lu.se/record/c9936668-1503-4a1e-8f4e-5f3ff34174bb
- author
- van der Oost, John ; von Wachenfeldt, Claes LU ; Hederstedt, Lars LU and Saraste, M
- organization
- publishing date
- 1991
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Molecular Microbiology
- volume
- 5
- issue
- 8
- pages
- 2063 - 2072
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:0025918198
- ISSN
- 1365-2958
- DOI
- 10.1111/j.1365-2958.1991.tb00829.x
- language
- English
- LU publication?
- yes
- id
- c9936668-1503-4a1e-8f4e-5f3ff34174bb
- date added to LUP
- 2017-07-18 10:36:36
- date last changed
- 2021-07-18 04:15:58
@article{c9936668-1503-4a1e-8f4e-5f3ff34174bb,
abstract = {{The ctaBCDEF genes coding for cytochrome c oxidase were found to reside adjacent to a regulatory gene ctaA at 127-degrees on the Bacillus subtilis chromosome. The structural genes for subunits I and II, ctaD and ctaC, were deleted by gene-replacement using a phleomycin-resistance marker. The mutant was unable to oxidize N,N,N',N'-tetramethyl-p-phenylenediamine and oxidized cytochrome c at a significantly lower rate. Absorption spectra of the mutant and wild-type membranes confirmed the presence of two haem A-containing enzymes in B. subtilis. Another mutant, with a spontaneous deletion upstream from ctaC, was found to express neither of these enzymes. Radioactive haem-labelling was used to identify subunit 11, which contains a haem C, and cytochrome c-550 among the membrane-bound c-type cytochromes of B. subtilis.}},
author = {{van der Oost, John and von Wachenfeldt, Claes and Hederstedt, Lars and Saraste, M}},
issn = {{1365-2958}},
language = {{eng}},
number = {{8}},
pages = {{2063--2072}},
publisher = {{Wiley-Blackwell}},
series = {{Molecular Microbiology}},
title = {{<em>Bacillus subtilis</em> cytochrome oxidase mutants: biochemical analysis and genetic evidence for two aa<sub>3</sub>-type oxidases}},
url = {{http://dx.doi.org/10.1111/j.1365-2958.1991.tb00829.x}},
doi = {{10.1111/j.1365-2958.1991.tb00829.x}},
volume = {{5}},
year = {{1991}},
}