The NF-κB-modulated miR-19a-3p enhances malignancy of human ovarian cancer cells through inhibition of IGFBP-3 expression
(2019) In Molecular Carcinogenesis 58(12). p.2254-2265- Abstract
Ovarian cancer is the most lethal gynecologic malignancy due to the lack of symptoms until advanced stages, and new diagnosis and treatment strategy is in urgent need. In this study, we found higher expression of miR-19a-3p in ovarian cancer tissues compared with that in the adjacent normal tissues. By chromatin immunoprecipitation (ChIP) and electrophoretic mobility shift assay (EMSA) analysis, we showed that nuclear factor-kappaB (NF-κB) binds to the promoter of miR-19a-3p, leading to reduced expression in ovarian cancer cells. Further study indicated that miR-19a-3p inhibits the expression of insulin-like growth factor binding protein-3 (IGFBP-3), resulting in enhanced growth and migration of ovarian cancer cells in vitro and tumor... (More)
Ovarian cancer is the most lethal gynecologic malignancy due to the lack of symptoms until advanced stages, and new diagnosis and treatment strategy is in urgent need. In this study, we found higher expression of miR-19a-3p in ovarian cancer tissues compared with that in the adjacent normal tissues. By chromatin immunoprecipitation (ChIP) and electrophoretic mobility shift assay (EMSA) analysis, we showed that nuclear factor-kappaB (NF-κB) binds to the promoter of miR-19a-3p, leading to reduced expression in ovarian cancer cells. Further study indicated that miR-19a-3p inhibits the expression of insulin-like growth factor binding protein-3 (IGFBP-3), resulting in enhanced growth and migration of ovarian cancer cells in vitro and tumor growth in vivo. These results showed that miR-19a-3p enhances the oncogenesis of ovarian cancer through inhibition of IGFBP-3 expression, and which can be inhibited by NF-κB, suggesting an NF-κB/miR-19a-3p/IGFBP-3 pathway in the oncogenesis of ovarian cancer, which expands our understanding of ovarian cancer and they may contribute to the development of new diagnosis and treatment of ovarian cancer.
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- author
- Bai, Ru ; Cui, Zhenhua ; Ma, Yongjing ; Wu, Yang ; Wang, Ningping ; Huang, Ling ; Yao, Qing and Sun, Jianmin LU
- organization
- publishing date
- 2019-12
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- IGFBP-3, miR-19a-3p, NF-κB, ovarian cancer
- in
- Molecular Carcinogenesis
- volume
- 58
- issue
- 12
- pages
- 12 pages
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- scopus:85073789803
- pmid:31513316
- ISSN
- 0899-1987
- DOI
- 10.1002/mc.23113
- language
- English
- LU publication?
- yes
- id
- d3234bd5-92c8-4cd5-9655-d1551f4af997
- date added to LUP
- 2019-11-01 11:30:50
- date last changed
- 2024-07-10 05:10:43
@article{d3234bd5-92c8-4cd5-9655-d1551f4af997,
abstract = {{<p>Ovarian cancer is the most lethal gynecologic malignancy due to the lack of symptoms until advanced stages, and new diagnosis and treatment strategy is in urgent need. In this study, we found higher expression of miR-19a-3p in ovarian cancer tissues compared with that in the adjacent normal tissues. By chromatin immunoprecipitation (ChIP) and electrophoretic mobility shift assay (EMSA) analysis, we showed that nuclear factor-kappaB (NF-κB) binds to the promoter of miR-19a-3p, leading to reduced expression in ovarian cancer cells. Further study indicated that miR-19a-3p inhibits the expression of insulin-like growth factor binding protein-3 (IGFBP-3), resulting in enhanced growth and migration of ovarian cancer cells in vitro and tumor growth in vivo. These results showed that miR-19a-3p enhances the oncogenesis of ovarian cancer through inhibition of IGFBP-3 expression, and which can be inhibited by NF-κB, suggesting an NF-κB/miR-19a-3p/IGFBP-3 pathway in the oncogenesis of ovarian cancer, which expands our understanding of ovarian cancer and they may contribute to the development of new diagnosis and treatment of ovarian cancer.</p>}},
author = {{Bai, Ru and Cui, Zhenhua and Ma, Yongjing and Wu, Yang and Wang, Ningping and Huang, Ling and Yao, Qing and Sun, Jianmin}},
issn = {{0899-1987}},
keywords = {{IGFBP-3; miR-19a-3p; NF-κB; ovarian cancer}},
language = {{eng}},
number = {{12}},
pages = {{2254--2265}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Molecular Carcinogenesis}},
title = {{The NF-κB-modulated miR-19a-3p enhances malignancy of human ovarian cancer cells through inhibition of IGFBP-3 expression}},
url = {{http://dx.doi.org/10.1002/mc.23113}},
doi = {{10.1002/mc.23113}},
volume = {{58}},
year = {{2019}},
}