Identification and characterization of the ccdA gene, required for cytochrome c synthesis in Bacillus subtilis
(1997) In Journal of Bacteriology 179(6). p.1962-1973- Abstract
- The gram-positive, endospore-forming bacterium Bacillus subtilis contains several membrane-bound c-type cytochromes. We have isolated a mutant pleiotropically deficient in cytochromes c. The responsible mutation resides in a gene which we have named ccdA (cytochrome c defective). This gene is located at 173 degrees on the B. subtilis chromosome. The ccdA gene was found to be specifically required for synthesis of cytochromes of the c type. CcdA is a predicted 26-kDa integral membrane protein with no clear similarity to any known cytochrome c biogenesis protein but seems to be related to a part of Escherichia coli DipZ/DsbD. The ccdA gene is cotranscribed with two other genes. These genes encode a putative 13.5-kDa single-domain response... (More)
- The gram-positive, endospore-forming bacterium Bacillus subtilis contains several membrane-bound c-type cytochromes. We have isolated a mutant pleiotropically deficient in cytochromes c. The responsible mutation resides in a gene which we have named ccdA (cytochrome c defective). This gene is located at 173 degrees on the B. subtilis chromosome. The ccdA gene was found to be specifically required for synthesis of cytochromes of the c type. CcdA is a predicted 26-kDa integral membrane protein with no clear similarity to any known cytochrome c biogenesis protein but seems to be related to a part of Escherichia coli DipZ/DsbD. The ccdA gene is cotranscribed with two other genes. These genes encode a putative 13.5-kDa single-domain response regulator, similar to B. subtilis CheY and SpoOF, and a predicted 18-kDa hydrophobic protein with no similarity to any protein in databases, respectively. Inactivation of the three genes showed that only ccdA is required for cytochrome c synthesis. The results also demonstrated that cytochromes of the c type are not needed for growth of B. subtilis. (Less)
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- author
- Schiött, Torbjörn LU ; von Wachenfeldt, Claes LU and Hederstedt, Lars LU
- organization
- publishing date
- 1997
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Bacteriology
- volume
- 179
- issue
- 6
- pages
- 1962 - 1973
- publisher
- American Society for Microbiology
- external identifiers
-
- scopus:0030897419
- ISSN
- 0021-9193
- DOI
- 10.1128/jb.179.6.1962-1973.1997
- language
- English
- LU publication?
- yes
- id
- d9deeef7-e7ac-4622-b67d-f3efe6e080a0
- date added to LUP
- 2017-07-18 10:02:33
- date last changed
- 2024-04-14 14:19:33
@article{d9deeef7-e7ac-4622-b67d-f3efe6e080a0, abstract = {{The gram-positive, endospore-forming bacterium Bacillus subtilis contains several membrane-bound c-type cytochromes. We have isolated a mutant pleiotropically deficient in cytochromes c. The responsible mutation resides in a gene which we have named ccdA (cytochrome c defective). This gene is located at 173 degrees on the B. subtilis chromosome. The ccdA gene was found to be specifically required for synthesis of cytochromes of the c type. CcdA is a predicted 26-kDa integral membrane protein with no clear similarity to any known cytochrome c biogenesis protein but seems to be related to a part of Escherichia coli DipZ/DsbD. The ccdA gene is cotranscribed with two other genes. These genes encode a putative 13.5-kDa single-domain response regulator, similar to B. subtilis CheY and SpoOF, and a predicted 18-kDa hydrophobic protein with no similarity to any protein in databases, respectively. Inactivation of the three genes showed that only ccdA is required for cytochrome c synthesis. The results also demonstrated that cytochromes of the c type are not needed for growth of B. subtilis.}}, author = {{Schiött, Torbjörn and von Wachenfeldt, Claes and Hederstedt, Lars}}, issn = {{0021-9193}}, language = {{eng}}, number = {{6}}, pages = {{1962--1973}}, publisher = {{American Society for Microbiology}}, series = {{Journal of Bacteriology}}, title = {{Identification and characterization of the<em> ccdA</em> gene, required for cytochrome c synthesis in <em>Bacillus subtilis</em>}}, url = {{http://dx.doi.org/10.1128/jb.179.6.1962-1973.1997}}, doi = {{10.1128/jb.179.6.1962-1973.1997}}, volume = {{179}}, year = {{1997}}, }