Quantitative proteomic characterization of lung-MSC and bone marrow-MSC using DIA-mass spectrometry
(2017) In Scientific Reports 7(1).- Abstract
Mesenchymal stromal cells (MSC) are ideal candidates for cell therapies, due to their immune-regulatory and regenerative properties. We have previously reported that lung-derived MSC are tissue-resident cells with lung-specific properties compared to bone marrow-derived MSC. Assessing relevant molecular differences between lung-MSC and bone marrow-MSC is important, given that such differences may impact their behavior and potential therapeutic use. Here, we present an in-depth mass spectrometry (MS) based strategy to investigate the proteomes of lung-MSC and bone marrow-MSC. The MS-strategy relies on label free quantitative data-independent acquisition (DIA) analysis and targeted data analysis using a MSC specific spectral library. We... (More)
Mesenchymal stromal cells (MSC) are ideal candidates for cell therapies, due to their immune-regulatory and regenerative properties. We have previously reported that lung-derived MSC are tissue-resident cells with lung-specific properties compared to bone marrow-derived MSC. Assessing relevant molecular differences between lung-MSC and bone marrow-MSC is important, given that such differences may impact their behavior and potential therapeutic use. Here, we present an in-depth mass spectrometry (MS) based strategy to investigate the proteomes of lung-MSC and bone marrow-MSC. The MS-strategy relies on label free quantitative data-independent acquisition (DIA) analysis and targeted data analysis using a MSC specific spectral library. We identified several significantly differentially expressed proteins between lung-MSC and bone marrow-MSC within the cell layer (352 proteins) and in the conditioned medium (49 proteins). Bioinformatics analysis revealed differences in regulation of cell proliferation, which was functionally confirmed by decreasing proliferation rate through Cytochrome P450 stimulation. Our study reveals important differences within proteome and matrisome profiles between lung- and bone marrow-derived MSC that may influence their behavior and affect the clinical outcome when used for cell-therapy.
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- author
- Rolandsson Enes, Sara LU ; Åhrman, Emma LU ; Palani, Anitha ; Hallgren, Oskar LU ; Bjermer, Leif LU ; Malmström, Anders LU ; Scheding, Stefan LU ; Malmström, Johan LU and Westergren-Thorsson, Gunilla LU
- organization
- publishing date
- 2017-08-24
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Journal Article
- in
- Scientific Reports
- volume
- 7
- issue
- 1
- article number
- 9316
- publisher
- Nature Publishing Group
- external identifiers
-
- scopus:85028076535
- wos:000408441600040
- pmid:28839187
- ISSN
- 2045-2322
- DOI
- 10.1038/s41598-017-09127-y
- language
- English
- LU publication?
- yes
- id
- eee65450-6f88-4664-a5ca-1ab378efe401
- date added to LUP
- 2017-09-04 17:11:06
- date last changed
- 2024-08-05 03:57:42
@article{eee65450-6f88-4664-a5ca-1ab378efe401, abstract = {{<p>Mesenchymal stromal cells (MSC) are ideal candidates for cell therapies, due to their immune-regulatory and regenerative properties. We have previously reported that lung-derived MSC are tissue-resident cells with lung-specific properties compared to bone marrow-derived MSC. Assessing relevant molecular differences between lung-MSC and bone marrow-MSC is important, given that such differences may impact their behavior and potential therapeutic use. Here, we present an in-depth mass spectrometry (MS) based strategy to investigate the proteomes of lung-MSC and bone marrow-MSC. The MS-strategy relies on label free quantitative data-independent acquisition (DIA) analysis and targeted data analysis using a MSC specific spectral library. We identified several significantly differentially expressed proteins between lung-MSC and bone marrow-MSC within the cell layer (352 proteins) and in the conditioned medium (49 proteins). Bioinformatics analysis revealed differences in regulation of cell proliferation, which was functionally confirmed by decreasing proliferation rate through Cytochrome P450 stimulation. Our study reveals important differences within proteome and matrisome profiles between lung- and bone marrow-derived MSC that may influence their behavior and affect the clinical outcome when used for cell-therapy.</p>}}, author = {{Rolandsson Enes, Sara and Åhrman, Emma and Palani, Anitha and Hallgren, Oskar and Bjermer, Leif and Malmström, Anders and Scheding, Stefan and Malmström, Johan and Westergren-Thorsson, Gunilla}}, issn = {{2045-2322}}, keywords = {{Journal Article}}, language = {{eng}}, month = {{08}}, number = {{1}}, publisher = {{Nature Publishing Group}}, series = {{Scientific Reports}}, title = {{Quantitative proteomic characterization of lung-MSC and bone marrow-MSC using DIA-mass spectrometry}}, url = {{http://dx.doi.org/10.1038/s41598-017-09127-y}}, doi = {{10.1038/s41598-017-09127-y}}, volume = {{7}}, year = {{2017}}, }