Atorvastatin activates PPAR-γ and attenuates the inflammatory response in human monocytes
(2002) In Inflammation Research 51(2). p.58-62- Abstract
Objective: To investigate the ability of statins to activate the nuclear receptor peroxisome proliferator-activated receptor gamma (PPAR-γ) in primary human monocytes in culture. Materials and methods: Human peripheral monocytes were incubated with atorvastatin (0.1-10 μmol/1) for up to 24 hours. PPAR-γ expression was analysed by electrophoretic mobility shift assay. Pro-inflammatory cytokines were measured by enzyme-linked immunosorbent assays, and oxygen consumption was determined polarographically with a Clark-type oxygen electrode. Results: We found that atorvastatin activates PPAR-γ and inhibits the production of tumour necrosis factor-alpha up to 38% (p < 0.05), monocyte chemoattractant protein-1 up to 85% (p < 0.05), and... (More)
Objective: To investigate the ability of statins to activate the nuclear receptor peroxisome proliferator-activated receptor gamma (PPAR-γ) in primary human monocytes in culture. Materials and methods: Human peripheral monocytes were incubated with atorvastatin (0.1-10 μmol/1) for up to 24 hours. PPAR-γ expression was analysed by electrophoretic mobility shift assay. Pro-inflammatory cytokines were measured by enzyme-linked immunosorbent assays, and oxygen consumption was determined polarographically with a Clark-type oxygen electrode. Results: We found that atorvastatin activates PPAR-γ and inhibits the production of tumour necrosis factor-alpha up to 38% (p < 0.05), monocyte chemoattractant protein-1 up to 85% (p < 0.05), and gelatinase B up to 73% (p < 0.05), in a concentration-dependent manner. Moreover, atorvastatin shows concentration-dependent inhibition of cellular oxygen consumption up to 41%. Conclusions: These findings contribute to the growing knowledge of the anti-inflammatory effects of statins, and have led us to the suggestion that statins may control inflammatory responses by the regulation of intracellular lipid homeostasis.
(Less)
- author
- Grip, Olof LU ; Janciauskiene, S. LU and Lindgren, S. LU
- organization
- publishing date
- 2002-02
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Atorvastatin, Inflammation, Monocytes, PPAR-γ
- in
- Inflammation Research
- volume
- 51
- issue
- 2
- pages
- 58 - 62
- publisher
- Birkhäuser
- external identifiers
-
- scopus:0036186165
- pmid:11926313
- ISSN
- 1023-3830
- DOI
- 10.1007/BF02684000
- language
- English
- LU publication?
- yes
- id
- f3aad159-89d5-4708-b006-68530bfc09f3
- date added to LUP
- 2019-08-07 13:29:27
- date last changed
- 2024-06-11 22:48:43
@article{f3aad159-89d5-4708-b006-68530bfc09f3, abstract = {{<p>Objective: To investigate the ability of statins to activate the nuclear receptor peroxisome proliferator-activated receptor gamma (PPAR-γ) in primary human monocytes in culture. Materials and methods: Human peripheral monocytes were incubated with atorvastatin (0.1-10 μmol/1) for up to 24 hours. PPAR-γ expression was analysed by electrophoretic mobility shift assay. Pro-inflammatory cytokines were measured by enzyme-linked immunosorbent assays, and oxygen consumption was determined polarographically with a Clark-type oxygen electrode. Results: We found that atorvastatin activates PPAR-γ and inhibits the production of tumour necrosis factor-alpha up to 38% (p < 0.05), monocyte chemoattractant protein-1 up to 85% (p < 0.05), and gelatinase B up to 73% (p < 0.05), in a concentration-dependent manner. Moreover, atorvastatin shows concentration-dependent inhibition of cellular oxygen consumption up to 41%. Conclusions: These findings contribute to the growing knowledge of the anti-inflammatory effects of statins, and have led us to the suggestion that statins may control inflammatory responses by the regulation of intracellular lipid homeostasis.</p>}}, author = {{Grip, Olof and Janciauskiene, S. and Lindgren, S.}}, issn = {{1023-3830}}, keywords = {{Atorvastatin; Inflammation; Monocytes; PPAR-γ}}, language = {{eng}}, number = {{2}}, pages = {{58--62}}, publisher = {{Birkhäuser}}, series = {{Inflammation Research}}, title = {{Atorvastatin activates PPAR-γ and attenuates the inflammatory response in human monocytes}}, url = {{http://dx.doi.org/10.1007/BF02684000}}, doi = {{10.1007/BF02684000}}, volume = {{51}}, year = {{2002}}, }