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A Probing Feeding Strategy for Escherichia coli Cultures

Axelsson, Jan Peter; Åkesson, Mats LU and Hagander, Per LU (1999) In Biotechnology Techniques 13. p.523-528
Abstract
A strain-independent feeding strategy for fed-batch cultures of <em>Escherichia coli</em> is presented. The key idea is to exploit a characteristic change in the cell metabolism at the onset of acetate formation. By superimposing short pulses in the glucose feed rate, on-line detection of acetate formation can be made using a standard dissolved oxygen sensor. A simple feedback algorithm is then used to adjust the feed rate to avoid acetate formation. The feasibility of the strategy is demonstrated by both simulation and experiments.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Escherichia coli, fed-batch culture, glucose, acetate, feedback control, dissolved oxygen.
in
Biotechnology Techniques
volume
13
pages
523 - 528
publisher
Springer
ISSN
0951-208X
language
English
LU publication?
yes
id
99cc0e2d-5c9d-4926-b8a4-f1653b98082e (old id 8497617)
date added to LUP
2015-12-26 16:15:07
date last changed
2016-04-16 12:43:08
@misc{99cc0e2d-5c9d-4926-b8a4-f1653b98082e,
  abstract     = {A strain-independent feeding strategy for fed-batch cultures of &lt;em&gt;Escherichia coli&lt;/em&gt; is presented. The key idea is to exploit a characteristic change in the cell metabolism at the onset of acetate formation. By superimposing short pulses in the glucose feed rate, on-line detection of acetate formation can be made using a standard dissolved oxygen sensor. A simple feedback algorithm is then used to adjust the feed rate to avoid acetate formation. The feasibility of the strategy is demonstrated by both simulation and experiments.},
  author       = {Axelsson, Jan Peter and Åkesson, Mats and Hagander, Per},
  issn         = {0951-208X},
  keyword      = {Escherichia coli,fed-batch culture,glucose,acetate,feedback control,dissolved oxygen.},
  language     = {eng},
  pages        = {523--528},
  publisher    = {ARRAY(0x9da2b20)},
  series       = {Biotechnology Techniques},
  title        = {A Probing Feeding Strategy for <em>Escherichia coli</em> Cultures},
  volume       = {13},
  year         = {1999},
}