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Functional diversity of LIM proteins : amino-terminal activation domains in the oncogenic proteins RBTN1 and RBTN2

Sánchez-García, I; Axelson, H LU and Rabbitts, T H (1995) In Oncogene 10(7). p.6-1301
Abstract

The RBTN1 and RBTN2 genes are activated by distinct translocations involving chromosome 11 in some T cell acute leukaemias. The RBTN proteins belong to the LIM family which comprises proteins with one, two or three cysteine-rich LIM domains, sometimes together with homeodomains or protein kinase domains. The RBTN1 and RBTN2 proteins comprise only tandem LIM domains. We report that RBTN1 and RBTN2 proteins are capable of supporting transcriptional transactivation of specific reporter genes in transfection assays. The results, using intact proteins or fusions with the homeodomain of the heterologous protein Isl-1, show that this transcriptional activation ability resides in the NH2-terminal parts of both proteins. The use of yeast assays... (More)

The RBTN1 and RBTN2 genes are activated by distinct translocations involving chromosome 11 in some T cell acute leukaemias. The RBTN proteins belong to the LIM family which comprises proteins with one, two or three cysteine-rich LIM domains, sometimes together with homeodomains or protein kinase domains. The RBTN1 and RBTN2 proteins comprise only tandem LIM domains. We report that RBTN1 and RBTN2 proteins are capable of supporting transcriptional transactivation of specific reporter genes in transfection assays. The results, using intact proteins or fusions with the homeodomain of the heterologous protein Isl-1, show that this transcriptional activation ability resides in the NH2-terminal parts of both proteins. The use of yeast assays with RBTN2 shows that RBTN2 forms homodimers and that the NH2-terminal 27 amino acids are sufficient to facilitate transcriptional transactivation. These data expand the functional diversity of the LIM-domain protein family and they augment the previously defined relationship between chromosomal translocations and transcriptional activation.

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organization
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type
Contribution to journal
publication status
published
subject
keywords
Adaptor Proteins, Signal Transducing, Amino Acid Sequence, DNA-Binding Proteins, Humans, LIM Domain Proteins, Metalloproteins, Molecular Sequence Data, Mutagenesis, Site-Directed, Oligodeoxyribonucleotides, Oncogene Proteins, Proto-Oncogene Proteins, Saccharomyces cerevisiae, Structure-Activity Relationship, Trans-Activators, Transcription Factors, Transcriptional Activation
in
Oncogene
volume
10
issue
7
pages
6 pages
publisher
Nature Publishing Group
external identifiers
  • Scopus:0028914486
ISSN
0950-9232
language
English
LU publication?
yes
id
b20d098f-07fe-42a5-9c7f-e833b03256ee
date added to LUP
2016-08-09 09:14:13
date last changed
2016-10-13 05:12:05
@misc{b20d098f-07fe-42a5-9c7f-e833b03256ee,
  abstract     = {<p>The RBTN1 and RBTN2 genes are activated by distinct translocations involving chromosome 11 in some T cell acute leukaemias. The RBTN proteins belong to the LIM family which comprises proteins with one, two or three cysteine-rich LIM domains, sometimes together with homeodomains or protein kinase domains. The RBTN1 and RBTN2 proteins comprise only tandem LIM domains. We report that RBTN1 and RBTN2 proteins are capable of supporting transcriptional transactivation of specific reporter genes in transfection assays. The results, using intact proteins or fusions with the homeodomain of the heterologous protein Isl-1, show that this transcriptional activation ability resides in the NH2-terminal parts of both proteins. The use of yeast assays with RBTN2 shows that RBTN2 forms homodimers and that the NH2-terminal 27 amino acids are sufficient to facilitate transcriptional transactivation. These data expand the functional diversity of the LIM-domain protein family and they augment the previously defined relationship between chromosomal translocations and transcriptional activation.</p>},
  author       = {Sánchez-García, I and Axelson, H and Rabbitts, T H},
  issn         = {0950-9232},
  keyword      = {Adaptor Proteins, Signal Transducing,Amino Acid Sequence,DNA-Binding Proteins,Humans,LIM Domain Proteins,Metalloproteins,Molecular Sequence Data,Mutagenesis, Site-Directed,Oligodeoxyribonucleotides,Oncogene Proteins,Proto-Oncogene Proteins,Saccharomyces cerevisiae,Structure-Activity Relationship,Trans-Activators,Transcription Factors,Transcriptional Activation},
  language     = {eng},
  month        = {04},
  number       = {7},
  pages        = {6--1301},
  publisher    = {ARRAY(0xb59c0e0)},
  series       = {Oncogene},
  title        = {Functional diversity of LIM proteins : amino-terminal activation domains in the oncogenic proteins RBTN1 and RBTN2},
  volume       = {10},
  year         = {1995},
}