Modelling and simulation of antibody purification
(2014) KET920 20142Department of Chemistry
Chemical Engineering (M.Sc.Eng.)
- Abstract
- Due to their high specificity against target antigens, monoclonal antibodies (mAbs) have proven to be a very powerful tool for diagnostic and therapeutic purpose against several diseases. Currently there are over 26 mAb approved for therapeutic use by the FDA. The manufacture of mAbs is a complex process where purification plays a key role. This project proposes and simulates a purification process based on an array of chromatographic columns. The different employed columns were modelled by kinetic dispersive models using parameters from available bibliography as well as some assumptions. The proposed method consists of five columns connected in series and offered arguably good results with a purity and yield of the target mAb of~90% and... (More)
- Due to their high specificity against target antigens, monoclonal antibodies (mAbs) have proven to be a very powerful tool for diagnostic and therapeutic purpose against several diseases. Currently there are over 26 mAb approved for therapeutic use by the FDA. The manufacture of mAbs is a complex process where purification plays a key role. This project proposes and simulates a purification process based on an array of chromatographic columns. The different employed columns were modelled by kinetic dispersive models using parameters from available bibliography as well as some assumptions. The proposed method consists of five columns connected in series and offered arguably good results with a purity and yield of the target mAb of~90% and ~85% respectively. (Less)
- Popular Abstract
- Monoclonal antibodies are a group of molecules with growing interest in the pharmaceutical industry. The different variations of this biomolecules are capable of identifying and binding to a wide variety of targets such as viruses, cancer cells or other agents causing diseases. This makes monoclonal antibodies a very powerful tool for diagnosis and therapy of diseases.
One of the critical steps in the manufacture of monoclonal antibodies is the purification process. The purpose of this work is to define a purification process and simulate it to predict the expected results.
In order to purify the target antibody it must be separated from the impurities present in the initial solution, which is achieved by using chromatographic columns... (More) - Monoclonal antibodies are a group of molecules with growing interest in the pharmaceutical industry. The different variations of this biomolecules are capable of identifying and binding to a wide variety of targets such as viruses, cancer cells or other agents causing diseases. This makes monoclonal antibodies a very powerful tool for diagnosis and therapy of diseases.
One of the critical steps in the manufacture of monoclonal antibodies is the purification process. The purpose of this work is to define a purification process and simulate it to predict the expected results.
In order to purify the target antibody it must be separated from the impurities present in the initial solution, which is achieved by using chromatographic columns which retain the different components more or less time depending on physical and chemical interactions, this means that some components will leave the column earlier while others will take longer allowing us the separate the target antibody from the impurities.
These different columns separate the components on the basis of their different charge, affinity to bind to a biomolecule and the different size of the components.
The simulated purification process consists of five different columns connected in series where the outflow of one column continuously feeds the next one.
This simulation showed promising results as over 90% purity was reached with a high yield of product and there is margin to refine the process. (Less)
Please use this url to cite or link to this publication:
http://lup.lub.lu.se/student-papers/record/4865142
- author
- Perez Serrano, Ferran LU
- supervisor
- organization
- course
- KET920 20142
- year
- 2014
- type
- H2 - Master's Degree (Two Years)
- subject
- keywords
- Chromatography, Protein A, Cationic Ion Exchange Chromatography, Antibody Purification.
- language
- English
- id
- 4865142
- date added to LUP
- 2014-12-19 12:36:45
- date last changed
- 2014-12-19 12:36:45
@misc{4865142, abstract = {{Due to their high specificity against target antigens, monoclonal antibodies (mAbs) have proven to be a very powerful tool for diagnostic and therapeutic purpose against several diseases. Currently there are over 26 mAb approved for therapeutic use by the FDA. The manufacture of mAbs is a complex process where purification plays a key role. This project proposes and simulates a purification process based on an array of chromatographic columns. The different employed columns were modelled by kinetic dispersive models using parameters from available bibliography as well as some assumptions. The proposed method consists of five columns connected in series and offered arguably good results with a purity and yield of the target mAb of~90% and ~85% respectively.}}, author = {{Perez Serrano, Ferran}}, language = {{eng}}, note = {{Student Paper}}, title = {{Modelling and simulation of antibody purification}}, year = {{2014}}, }