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The quantification of zebrafish ocular-associated proteins provides hints for sex-biased visual impairments and perception

Niksirat, Hamid LU orcid ; Siino, Valentina LU ; Steinbach, Christoph and Levander, Fredrik LU (2024) In Heliyon 10(12).
Abstract

Biochemical differences between sexes can also be seen in non-sexual organs and may affect organ functions and susceptibility to diseases. It has been shown that there are sex-biased visual perceptions and impairments. Abundance differences of eye proteins could provide explanations for some of these. Exploration of the ocular proteome was performed to find sex-based protein abundance differences in zebrafish Danio rerio. A label-free protein quantification workflow using high-resolution mass spectrometry was employed to find proteins with significant differences between the sexes. In total, 3740 unique master proteins were identified and quantified, and 49 proteins showed significant abundance differences between the eyes of male and... (More)

Biochemical differences between sexes can also be seen in non-sexual organs and may affect organ functions and susceptibility to diseases. It has been shown that there are sex-biased visual perceptions and impairments. Abundance differences of eye proteins could provide explanations for some of these. Exploration of the ocular proteome was performed to find sex-based protein abundance differences in zebrafish Danio rerio. A label-free protein quantification workflow using high-resolution mass spectrometry was employed to find proteins with significant differences between the sexes. In total, 3740 unique master proteins were identified and quantified, and 49 proteins showed significant abundance differences between the eyes of male and female zebrafish. Those proteins belong to lipoproteins, immune system, blood coagulation, antioxidants, iron and heme-binding proteins, ion channels, pumps and exchangers, neuronal and photoreceptor proteins, and the cytoskeleton. An extensive literature review provided clues for the possible links between the sex-biased level of proteins and visual perception and impairments. In conclusion, sexual dimorphism at the protein level was discovered for the first time in the eye of zebrafish and should be accounted for in ophthalmological studies. Data are available via ProteomeXchange with identifier PXD033338.

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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Eye, Protein, Proteomics, Sexual dimorphism
in
Heliyon
volume
10
issue
12
article number
e33057
publisher
Elsevier
external identifiers
  • scopus:85196143848
  • pmid:38994070
ISSN
2405-8440
DOI
10.1016/j.heliyon.2024.e33057
language
English
LU publication?
yes
id
00eff8d5-e6be-4b1e-959a-3751b1e8f8dd
date added to LUP
2024-08-19 13:56:33
date last changed
2024-08-20 03:00:03
@article{00eff8d5-e6be-4b1e-959a-3751b1e8f8dd,
  abstract     = {{<p>Biochemical differences between sexes can also be seen in non-sexual organs and may affect organ functions and susceptibility to diseases. It has been shown that there are sex-biased visual perceptions and impairments. Abundance differences of eye proteins could provide explanations for some of these. Exploration of the ocular proteome was performed to find sex-based protein abundance differences in zebrafish Danio rerio. A label-free protein quantification workflow using high-resolution mass spectrometry was employed to find proteins with significant differences between the sexes. In total, 3740 unique master proteins were identified and quantified, and 49 proteins showed significant abundance differences between the eyes of male and female zebrafish. Those proteins belong to lipoproteins, immune system, blood coagulation, antioxidants, iron and heme-binding proteins, ion channels, pumps and exchangers, neuronal and photoreceptor proteins, and the cytoskeleton. An extensive literature review provided clues for the possible links between the sex-biased level of proteins and visual perception and impairments. In conclusion, sexual dimorphism at the protein level was discovered for the first time in the eye of zebrafish and should be accounted for in ophthalmological studies. Data are available via ProteomeXchange with identifier PXD033338.</p>}},
  author       = {{Niksirat, Hamid and Siino, Valentina and Steinbach, Christoph and Levander, Fredrik}},
  issn         = {{2405-8440}},
  keywords     = {{Eye; Protein; Proteomics; Sexual dimorphism}},
  language     = {{eng}},
  month        = {{06}},
  number       = {{12}},
  publisher    = {{Elsevier}},
  series       = {{Heliyon}},
  title        = {{The quantification of zebrafish ocular-associated proteins provides hints for sex-biased visual impairments and perception}},
  url          = {{http://dx.doi.org/10.1016/j.heliyon.2024.e33057}},
  doi          = {{10.1016/j.heliyon.2024.e33057}},
  volume       = {{10}},
  year         = {{2024}},
}