Distribution of dopamine-immunoreactive neuronal perikarya and fibres in the brain of a teleost, gasterosteus aculeatus L. Comparison with tyrosine hydroxylase- and dopamine-β-hydroxylase-immunoreactive neurons
(1990) In Journal of Chemical Neuroanatomy 3(4). p.233-260- Abstract
The distribution of dopamine in the brain of the teleost Gasterosteus aculeatus L. was demonstrated with the indirect peroxidase-antiperoxidase immunohistochemical method using highly specific antibodies against a dopamine-glutaraldehyde-thyroglobulin conjugate. Dopamine-immunoreactive (DAir) neuronal somata were observed in all main brain regions. In the forebrain, DAir neurons were located in a continuous cell column extending from the caudal part of the olfactory bulbs to the preoptic area. The neurons lie lateral to the dorsal (and caudally to the subcommissural) portion of the ventral telencephalic area, and ventromedial to the central nuclei of the dorsal area. In the diencephalon, cerebrospinal fluid-contacting neurons were... (More)
The distribution of dopamine in the brain of the teleost Gasterosteus aculeatus L. was demonstrated with the indirect peroxidase-antiperoxidase immunohistochemical method using highly specific antibodies against a dopamine-glutaraldehyde-thyroglobulin conjugate. Dopamine-immunoreactive (DAir) neuronal somata were observed in all main brain regions. In the forebrain, DAir neurons were located in a continuous cell column extending from the caudal part of the olfactory bulbs to the preoptic area. The neurons lie lateral to the dorsal (and caudally to the subcommissural) portion of the ventral telencephalic area, and ventromedial to the central nuclei of the dorsal area. In the diencephalon, cerebrospinal fluid-contacting neurons were located in the paraventricular organ and in the subependymallayers of the dorsal and caudal zones of the periventricular hypothalamus. Small DAir neurons were observed in the suprachiasmatic nucleus, in the parvocellular preoptic nucleus and in the ventromedial thalamic nucleus, while large perikarya were observed dorsolateral to the dorsal zone of the periventricular hypothalamus ('PVO-accompanying cells'), in the posterior tuberal nucleus and in the most rostral portion of the mammillary bodies. Numerous small DAir neurons were located in the periventricular pretectal nucleus. In the brainstem, DAir neurons were observed in the isthmus region, in the dorsal raphe nucleus and in the lateral parts of the nucleus of the solitary tract. DAir perikarya were also observed in the area postrema. Direct comparison with the distribution of tyrosine hydroxylase- and dopamine-β- hydroxylaseimmunoreactivity (THir and DBHir) gave the following results: THir neurons were found in all areas where DAir neurons were located, except for the paraventricular organ and the dorsal and caudal zones of the periventricular hypothalamus, which were devoid of Thir. DBHir (putatively noradrenergic or adrenergic) neurons were observed in the lateral parts of the nucleus of the solitary tract, and in the isthmus region. The DBHir neurons in the isthmus region, which have previously been shown to be noradrenergic, appeared to be identical with the THir and DAir neurons of the same area. DAir axons were found in hihg numbers in most parts of the brain. Especially dense innervation was found in the ventrolateral and posterior parts of the dorsal telencephalic area, the region surrounding the lateral recesses of the third ventricle, the interpeduncular nucleus, the dorsal and median raphe nuclei (the rostral raphe nuclei), and in the nucleus of the solitary tract. The lowest densities were found in parts of the dorsal telencephalic area, the central nucleus of the inferior lobe, mammillary bodies, nucleus glomerulosus, dorsal thalamus, synencephalon, pretectum, torus longitudinalis, in valvula and corpus cerebelli and in cristae cerebellares. Like in previous studies of catecholaminergic systems in teleosts, the present study has not revealed DAir neurons in the midbrain tegmentum. In comparison with other vertebrates, the absence of differentiated mesencephalic dopaminergic nuclei-'substantia nigra' and 'ventral tegmental area'-is striking. The dense innervation of the ventrolateral and posterior parts of the dorsal telencephalic area raises the question whether the dopaminergic innervation of this area- the 'medial pallium' of teleost-has developed at the expense of a nigrostriatal system in the stickleback. However, the medial and central parts of the dorsal telencephalic area also receive a relatively prominent DAir innervation. Thus, other DAir neuronal groups may have developed 'striatal' projections in the stickleback.
(Less)
- author
- Ekström, Peter LU ; Honkanen, T. and Steinbusch, H. W M
- publishing date
- 1990-01-01
- type
- Contribution to journal
- publication status
- published
- in
- Journal of Chemical Neuroanatomy
- volume
- 3
- issue
- 4
- pages
- 28 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:1975745
- scopus:0025007999
- ISSN
- 0891-0618
- language
- English
- LU publication?
- no
- id
- 06464e34-0167-4bdb-bac1-f0d89c13257d
- date added to LUP
- 2019-10-02 15:41:04
- date last changed
- 2024-01-01 21:40:44
@article{06464e34-0167-4bdb-bac1-f0d89c13257d,
abstract = {{<p>The distribution of dopamine in the brain of the teleost Gasterosteus aculeatus L. was demonstrated with the indirect peroxidase-antiperoxidase immunohistochemical method using highly specific antibodies against a dopamine-glutaraldehyde-thyroglobulin conjugate. Dopamine-immunoreactive (DAir) neuronal somata were observed in all main brain regions. In the forebrain, DAir neurons were located in a continuous cell column extending from the caudal part of the olfactory bulbs to the preoptic area. The neurons lie lateral to the dorsal (and caudally to the subcommissural) portion of the ventral telencephalic area, and ventromedial to the central nuclei of the dorsal area. In the diencephalon, cerebrospinal fluid-contacting neurons were located in the paraventricular organ and in the subependymallayers of the dorsal and caudal zones of the periventricular hypothalamus. Small DAir neurons were observed in the suprachiasmatic nucleus, in the parvocellular preoptic nucleus and in the ventromedial thalamic nucleus, while large perikarya were observed dorsolateral to the dorsal zone of the periventricular hypothalamus ('PVO-accompanying cells'), in the posterior tuberal nucleus and in the most rostral portion of the mammillary bodies. Numerous small DAir neurons were located in the periventricular pretectal nucleus. In the brainstem, DAir neurons were observed in the isthmus region, in the dorsal raphe nucleus and in the lateral parts of the nucleus of the solitary tract. DAir perikarya were also observed in the area postrema. Direct comparison with the distribution of tyrosine hydroxylase- and dopamine-β- hydroxylaseimmunoreactivity (THir and DBHir) gave the following results: THir neurons were found in all areas where DAir neurons were located, except for the paraventricular organ and the dorsal and caudal zones of the periventricular hypothalamus, which were devoid of Thir. DBHir (putatively noradrenergic or adrenergic) neurons were observed in the lateral parts of the nucleus of the solitary tract, and in the isthmus region. The DBHir neurons in the isthmus region, which have previously been shown to be noradrenergic, appeared to be identical with the THir and DAir neurons of the same area. DAir axons were found in hihg numbers in most parts of the brain. Especially dense innervation was found in the ventrolateral and posterior parts of the dorsal telencephalic area, the region surrounding the lateral recesses of the third ventricle, the interpeduncular nucleus, the dorsal and median raphe nuclei (the rostral raphe nuclei), and in the nucleus of the solitary tract. The lowest densities were found in parts of the dorsal telencephalic area, the central nucleus of the inferior lobe, mammillary bodies, nucleus glomerulosus, dorsal thalamus, synencephalon, pretectum, torus longitudinalis, in valvula and corpus cerebelli and in cristae cerebellares. Like in previous studies of catecholaminergic systems in teleosts, the present study has not revealed DAir neurons in the midbrain tegmentum. In comparison with other vertebrates, the absence of differentiated mesencephalic dopaminergic nuclei-'substantia nigra' and 'ventral tegmental area'-is striking. The dense innervation of the ventrolateral and posterior parts of the dorsal telencephalic area raises the question whether the dopaminergic innervation of this area- the 'medial pallium' of teleost-has developed at the expense of a nigrostriatal system in the stickleback. However, the medial and central parts of the dorsal telencephalic area also receive a relatively prominent DAir innervation. Thus, other DAir neuronal groups may have developed 'striatal' projections in the stickleback.</p>}},
author = {{Ekström, Peter and Honkanen, T. and Steinbusch, H. W M}},
issn = {{0891-0618}},
language = {{eng}},
month = {{01}},
number = {{4}},
pages = {{233--260}},
publisher = {{Elsevier}},
series = {{Journal of Chemical Neuroanatomy}},
title = {{Distribution of dopamine-immunoreactive neuronal perikarya and fibres in the brain of a teleost, gasterosteus aculeatus L. Comparison with tyrosine hydroxylase- and dopamine-β-hydroxylase-immunoreactive neurons}},
volume = {{3}},
year = {{1990}},
}