Genetic Variation Within the HLA-DRA1 Gene Modulates Susceptibility to Type 1 Diabetes in HLA-DR3 Homozygotes
(2019) In Diabetes 68(7). p.1523-1527- Abstract
Type 1 diabetes (T1D) involves the interaction of multiple gene variants, environmental factors, and immunoregulatory dysfunction. Major T1D genetic risk loci encode HLA-DR and -DQ. Genetic heterogeneity and linkage disequilibrium in the highly polymorphic HLA region confound attempts to identify additional T1D susceptibility loci. To minimize HLA heterogeneity, T1D patients (N = 365) and control subjects (N = 668) homozygous for the HLA-DR3 high-risk haplotype were selected from multiple large T1D studies and examined to identify new T1D susceptibility loci using molecular inversion probe sequencing technology. We report that risk for T1D in HLA-DR3 homozygotes is increased significantly by a previously unreported haplotype of three... (More)
Type 1 diabetes (T1D) involves the interaction of multiple gene variants, environmental factors, and immunoregulatory dysfunction. Major T1D genetic risk loci encode HLA-DR and -DQ. Genetic heterogeneity and linkage disequilibrium in the highly polymorphic HLA region confound attempts to identify additional T1D susceptibility loci. To minimize HLA heterogeneity, T1D patients (N = 365) and control subjects (N = 668) homozygous for the HLA-DR3 high-risk haplotype were selected from multiple large T1D studies and examined to identify new T1D susceptibility loci using molecular inversion probe sequencing technology. We report that risk for T1D in HLA-DR3 homozygotes is increased significantly by a previously unreported haplotype of three single nucleotide polymorphisms (SNPs) within the first intron of HLA-DRA1. The homozygous risk haplotype has an odds ratio of 4.65 relative to the protective homozygous haplotype in our sample. Individually, these SNPs reportedly function as "expression quantitative trait loci," modulating HLA-DR and -DQ expression. From our analysis of available data, we conclude that the tri-SNP haplotype within HLA-DRA1 may modulate class II expression, suggesting that increased T1D risk could be attributable to regulated expression of class II genes. These findings could help clarify the role of HLA in T1D susceptibility and improve diabetes risk assessment, particularly in high-risk HLA-DR3 homozygous individuals.
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- author
- Aydemir, Özkan ; Noble, Janelle A ; Bailey, Jeffrey A ; Lernmark, Åke LU ; Marsh, Patrick ; Andersson Svärd, Agnes LU ; Bearoff, Frank ; Blankenhorn, Elizabeth P and Mordes, John P
- author collaboration
- organization
- publishing date
- 2019-07
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Diabetes
- volume
- 68
- issue
- 7
- pages
- 5 pages
- publisher
- American Diabetes Association Inc.
- external identifiers
-
- scopus:85068537598
- pmid:30962219
- ISSN
- 1939-327X
- DOI
- 10.2337/db18-1128
- project
- Immunological markers of type 1 diabetes pathogenesis prior to clinical diagnosis
- language
- English
- LU publication?
- yes
- additional info
- © 2019 by the American Diabetes Association.
- id
- 07d614ee-9e12-4d7a-8c27-8ad22469703f
- date added to LUP
- 2019-06-25 13:11:10
- date last changed
- 2024-04-02 12:01:26
@article{07d614ee-9e12-4d7a-8c27-8ad22469703f, abstract = {{<p>Type 1 diabetes (T1D) involves the interaction of multiple gene variants, environmental factors, and immunoregulatory dysfunction. Major T1D genetic risk loci encode HLA-DR and -DQ. Genetic heterogeneity and linkage disequilibrium in the highly polymorphic HLA region confound attempts to identify additional T1D susceptibility loci. To minimize HLA heterogeneity, T1D patients (N = 365) and control subjects (N = 668) homozygous for the HLA-DR3 high-risk haplotype were selected from multiple large T1D studies and examined to identify new T1D susceptibility loci using molecular inversion probe sequencing technology. We report that risk for T1D in HLA-DR3 homozygotes is increased significantly by a previously unreported haplotype of three single nucleotide polymorphisms (SNPs) within the first intron of HLA-DRA1. The homozygous risk haplotype has an odds ratio of 4.65 relative to the protective homozygous haplotype in our sample. Individually, these SNPs reportedly function as "expression quantitative trait loci," modulating HLA-DR and -DQ expression. From our analysis of available data, we conclude that the tri-SNP haplotype within HLA-DRA1 may modulate class II expression, suggesting that increased T1D risk could be attributable to regulated expression of class II genes. These findings could help clarify the role of HLA in T1D susceptibility and improve diabetes risk assessment, particularly in high-risk HLA-DR3 homozygous individuals.</p>}}, author = {{Aydemir, Özkan and Noble, Janelle A and Bailey, Jeffrey A and Lernmark, Åke and Marsh, Patrick and Andersson Svärd, Agnes and Bearoff, Frank and Blankenhorn, Elizabeth P and Mordes, John P}}, issn = {{1939-327X}}, language = {{eng}}, number = {{7}}, pages = {{1523--1527}}, publisher = {{American Diabetes Association Inc.}}, series = {{Diabetes}}, title = {{Genetic Variation Within the HLA-DRA1 Gene Modulates Susceptibility to Type 1 Diabetes in HLA-DR3 Homozygotes}}, url = {{http://dx.doi.org/10.2337/db18-1128}}, doi = {{10.2337/db18-1128}}, volume = {{68}}, year = {{2019}}, }