Kit regulates maintenance of quiescent hematopoietic stem cells.
(2008) In Journal of Immunology 180(4). p.2045-2053- Abstract
- Hematopoietic stem cell (HSC) numbers are tightly regulated and maintained in postnatal hematopoiesis. Extensive studies have supported a role of the cytokine tyrosine kinase receptor Kit in sustaining cycling HSCs when competing with wild-type HSCs posttransplantation, but not in maintenance of quiescent HSCs in steady state adult bone marrow. In this study, we investigated HSC regulation in White Spotting 41 (Kit(W41/W41)) mice, with a partial loss of function of Kit. Although the extensive fetal HSC expansion was Kit-independent, adult Kit(W41/W41) mice had an almost 2-fold reduction in long-term HSCs, reflecting a loss of roughly 10,000 Lin(-)Sca-1(+)Kit(high) (LSK)CD34(-)Flt3(-) long-term HSCs by 12 wk of age, whereas... (More)
- Hematopoietic stem cell (HSC) numbers are tightly regulated and maintained in postnatal hematopoiesis. Extensive studies have supported a role of the cytokine tyrosine kinase receptor Kit in sustaining cycling HSCs when competing with wild-type HSCs posttransplantation, but not in maintenance of quiescent HSCs in steady state adult bone marrow. In this study, we investigated HSC regulation in White Spotting 41 (Kit(W41/W41)) mice, with a partial loss of function of Kit. Although the extensive fetal HSC expansion was Kit-independent, adult Kit(W41/W41) mice had an almost 2-fold reduction in long-term HSCs, reflecting a loss of roughly 10,000 Lin(-)Sca-1(+)Kit(high) (LSK)CD34(-)Flt3(-) long-term HSCs by 12 wk of age, whereas LSKCD34(+)Flt3(-) short-term HSCs and LSKCD34(+)Flt3(+) multipotent progenitors were less affected. Whereas homing and initial reconstitution of Kit(W41/W41) bone marrow cells in myeloablated recipients were close to normal, self-renewing Kit(W41/W41) HSCs were progressively depleted in not only competitive but also noncompetitive transplantation assays. Overexpression of the anti-apoptotic regulator BCL-2 partially rescued the posttransplantation Kit(W41/W41) HSC deficiency, suggesting that Kit might at least in the posttransplantation setting in part sustain HSC numbers by promoting HSC survival. Most notably, accelerated in vivo BrdU incorporation and cell cycle kinetics implicated a previously unrecognized role of Kit in maintaining quiescent HSCs in steady state adult hematopoiesis. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1042212
- author
- Thorén, Lina LU ; Liuba, Karina LU ; Bryder, David LU ; Nygren, Jens LU ; Jensen, Christina LU ; Qian, Hong LU ; Antonchuk, Jennifer LU and Jacobsen, Sten Eirik W LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Immunology
- volume
- 180
- issue
- 4
- pages
- 2045 - 2053
- publisher
- American Association of Immunologists
- external identifiers
-
- pmid:18250409
- wos:000253005600013
- scopus:42149157791
- ISSN
- 1550-6606
- language
- English
- LU publication?
- yes
- id
- 4b7d9226-195d-439e-8b7e-4c56c0a24ea8 (old id 1042212)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/18250409?dopt=Abstract
- date added to LUP
- 2016-04-04 09:11:12
- date last changed
- 2022-08-08 17:10:50
@article{4b7d9226-195d-439e-8b7e-4c56c0a24ea8, abstract = {{Hematopoietic stem cell (HSC) numbers are tightly regulated and maintained in postnatal hematopoiesis. Extensive studies have supported a role of the cytokine tyrosine kinase receptor Kit in sustaining cycling HSCs when competing with wild-type HSCs posttransplantation, but not in maintenance of quiescent HSCs in steady state adult bone marrow. In this study, we investigated HSC regulation in White Spotting 41 (Kit(W41/W41)) mice, with a partial loss of function of Kit. Although the extensive fetal HSC expansion was Kit-independent, adult Kit(W41/W41) mice had an almost 2-fold reduction in long-term HSCs, reflecting a loss of roughly 10,000 Lin(-)Sca-1(+)Kit(high) (LSK)CD34(-)Flt3(-) long-term HSCs by 12 wk of age, whereas LSKCD34(+)Flt3(-) short-term HSCs and LSKCD34(+)Flt3(+) multipotent progenitors were less affected. Whereas homing and initial reconstitution of Kit(W41/W41) bone marrow cells in myeloablated recipients were close to normal, self-renewing Kit(W41/W41) HSCs were progressively depleted in not only competitive but also noncompetitive transplantation assays. Overexpression of the anti-apoptotic regulator BCL-2 partially rescued the posttransplantation Kit(W41/W41) HSC deficiency, suggesting that Kit might at least in the posttransplantation setting in part sustain HSC numbers by promoting HSC survival. Most notably, accelerated in vivo BrdU incorporation and cell cycle kinetics implicated a previously unrecognized role of Kit in maintaining quiescent HSCs in steady state adult hematopoiesis.}}, author = {{Thorén, Lina and Liuba, Karina and Bryder, David and Nygren, Jens and Jensen, Christina and Qian, Hong and Antonchuk, Jennifer and Jacobsen, Sten Eirik W}}, issn = {{1550-6606}}, language = {{eng}}, number = {{4}}, pages = {{2045--2053}}, publisher = {{American Association of Immunologists}}, series = {{Journal of Immunology}}, title = {{Kit regulates maintenance of quiescent hematopoietic stem cells.}}, url = {{http://www.ncbi.nlm.nih.gov/pubmed/18250409?dopt=Abstract}}, volume = {{180}}, year = {{2008}}, }