Protein Kinase Cepsilon Actin-binding Site Is Important for Neurite Outgrowth during Neuronal Differentiation.
(2002) In Molecular Biology of the Cell 13(1). p.12-24- Abstract
- We have previously shown that protein kinase Cepsilon (PKCepsilon) induces neurite outgrowth via its regulatory domain and independently of its kinase activity. This study aimed at identifying mechanisms regulating PKCepsilon-mediated neurite induction. We show an increased association of PKCepsilon to the cytoskeleton during neuronal differentiation. Furthermore, neurite induction by overexpression of full-length PKCepsilon is suppressed if serum is removed from the cultures or if an actin-binding site is deleted from the protein. A peptide corresponding to the PKCepsilon actin-binding site suppresses neurite outgrowth during neuronal differentiation and outgrowth elicited by PKCepsilon overexpression. Neither serum removal, deletion of... (More)
- We have previously shown that protein kinase Cepsilon (PKCepsilon) induces neurite outgrowth via its regulatory domain and independently of its kinase activity. This study aimed at identifying mechanisms regulating PKCepsilon-mediated neurite induction. We show an increased association of PKCepsilon to the cytoskeleton during neuronal differentiation. Furthermore, neurite induction by overexpression of full-length PKCepsilon is suppressed if serum is removed from the cultures or if an actin-binding site is deleted from the protein. A peptide corresponding to the PKCepsilon actin-binding site suppresses neurite outgrowth during neuronal differentiation and outgrowth elicited by PKCepsilon overexpression. Neither serum removal, deletion of the actin-binding site, nor introduction of the peptide affects neurite induction by the isolated regulatory domain. Membrane targeting by myristoylation renders full-length PKCepsilon independent of both serum and the actin-binding site, and PKCepsilon colocalized with F-actin at the cortical cytoskeleton during neurite outgrowth. These results demonstrate that the actin-binding site is of importance for signals acting on PKCepsilon in a pathway leading to neurite outgrowth. Localization of PKCepsilon to the plasma membrane and/or the cortical cytoskeleton is conceivably important for its effect on neurite outgrowth. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/106461
- author
- Zeidman, Ruth LU ; Trollér, Ulrika LU ; Raghunath, Arathi ; Påhlman, Sven LU and Larsson, Christer LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Image Cytometry, Microscopy, Confocal, Fluorescence, Neurites/*physiology, Neuroblastoma, Protein Conformation, Protein Kinase C/*chemistry/genetics/*metabolism, Recombinant Fusion Proteins/metabolism, Substrate Specificity, Support, Non-U.S. Gov't, Transfection, Cultured, Tumor Cells, Isoenzymes/*chemistry/genetics/*metabolism, Human, Cytoskeleton/metabolism, Cell Differentiation/physiology, Binding Sites, Actins/*metabolism
- in
- Molecular Biology of the Cell
- volume
- 13
- issue
- 1
- pages
- 12 - 24
- publisher
- American Society for Cell Biology
- external identifiers
-
- wos:000173402000002
- pmid:11809819
- scopus:0036007287
- ISSN
- 1939-4586
- DOI
- 10.1091/mbc.01-04-0210
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Tumour Cell Biology (013017530), Molecular Medicine (013031200), Division of Molecular Medicine and Gene Therapy (013022010)
- id
- e7050503-30b8-4232-9fad-6c1e1fc481dd (old id 106461)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11809819&dopt=Abstract
- date added to LUP
- 2016-04-01 11:35:03
- date last changed
- 2022-01-26 07:15:11
@article{e7050503-30b8-4232-9fad-6c1e1fc481dd, abstract = {{We have previously shown that protein kinase Cepsilon (PKCepsilon) induces neurite outgrowth via its regulatory domain and independently of its kinase activity. This study aimed at identifying mechanisms regulating PKCepsilon-mediated neurite induction. We show an increased association of PKCepsilon to the cytoskeleton during neuronal differentiation. Furthermore, neurite induction by overexpression of full-length PKCepsilon is suppressed if serum is removed from the cultures or if an actin-binding site is deleted from the protein. A peptide corresponding to the PKCepsilon actin-binding site suppresses neurite outgrowth during neuronal differentiation and outgrowth elicited by PKCepsilon overexpression. Neither serum removal, deletion of the actin-binding site, nor introduction of the peptide affects neurite induction by the isolated regulatory domain. Membrane targeting by myristoylation renders full-length PKCepsilon independent of both serum and the actin-binding site, and PKCepsilon colocalized with F-actin at the cortical cytoskeleton during neurite outgrowth. These results demonstrate that the actin-binding site is of importance for signals acting on PKCepsilon in a pathway leading to neurite outgrowth. Localization of PKCepsilon to the plasma membrane and/or the cortical cytoskeleton is conceivably important for its effect on neurite outgrowth.}}, author = {{Zeidman, Ruth and Trollér, Ulrika and Raghunath, Arathi and Påhlman, Sven and Larsson, Christer}}, issn = {{1939-4586}}, keywords = {{Image Cytometry; Microscopy; Confocal; Fluorescence; Neurites/*physiology; Neuroblastoma; Protein Conformation; Protein Kinase C/*chemistry/genetics/*metabolism; Recombinant Fusion Proteins/metabolism; Substrate Specificity; Support; Non-U.S. Gov't; Transfection; Cultured; Tumor Cells; Isoenzymes/*chemistry/genetics/*metabolism; Human; Cytoskeleton/metabolism; Cell Differentiation/physiology; Binding Sites; Actins/*metabolism}}, language = {{eng}}, number = {{1}}, pages = {{12--24}}, publisher = {{American Society for Cell Biology}}, series = {{Molecular Biology of the Cell}}, title = {{Protein Kinase Cepsilon Actin-binding Site Is Important for Neurite Outgrowth during Neuronal Differentiation.}}, url = {{http://dx.doi.org/10.1091/mbc.01-04-0210}}, doi = {{10.1091/mbc.01-04-0210}}, volume = {{13}}, year = {{2002}}, }