Endothelin-1 Reduces Microvascular Fluid Permeability through Secondary Release of Prostacyclin in Cat Skeletal Muscle.
(2002) In Microvascular Research 63(1). p.50-60- Abstract
- The aim of the study was to analyze effects of various plasma concentrations of the vasoconstrictor endothelin-1 on microvascular fluid permeability and on transcapillary fluid exchange. We also analyzed whether the permeability-reducing substance prostacyclin is involved in the permeability effects of endothelin-1, as prostacylin is suggested to be released via ET(B) receptor stimulation. The study was performed on an autoperfused cat calf muscle preparation, and a capillary filtration coefficient (CFC) technique was used to estimate variations in microvascular fluid permeability (conductivity). Intraarterial infusion of endothelin-1 in low doses (5 and 10 ng/min/100 g muscle) caused transcapillary absorption, whereas higher doses (20-40... (More)
- The aim of the study was to analyze effects of various plasma concentrations of the vasoconstrictor endothelin-1 on microvascular fluid permeability and on transcapillary fluid exchange. We also analyzed whether the permeability-reducing substance prostacyclin is involved in the permeability effects of endothelin-1, as prostacylin is suggested to be released via ET(B) receptor stimulation. The study was performed on an autoperfused cat calf muscle preparation, and a capillary filtration coefficient (CFC) technique was used to estimate variations in microvascular fluid permeability (conductivity). Intraarterial infusion of endothelin-1 in low doses (5 and 10 ng/min/100 g muscle) caused transcapillary absorption, whereas higher doses (20-40 ng/min/100 g) induced filtration despite further vasoconstriction. Low-dose endothelin-1 had no significant effect on CFC, while CFC was reduced to at most 55% of baseline at higher doses (P < 0.01). Simultaneous local intraarterial infusion of the prostacyclin synthesis inhibitor tranylcypromine restored CFC to 114% of baseline (P < 0.01) and further increased vascular resistance. A low, nonvasodilator dose of prostacyclin given intravenously counteracted the tranylcypromine effect on CFC. The decreased CFC induced by a high dose of endothelin-1 was counteracted by the ET(B) receptor antagonist BQ-788 with no change in vascular resistance (P < 0.05). We conclude that the decreased CFC following high doses of endothelin-1 can be attributed to a decrease in microvascular hydraulic conductivity, mediated by secondary release of prostacylin via stimulation of the ET(B) receptor. Endothelin-1 may induce edema through postcapillary vasoconstriction. (c)2001 Elsevier Science. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/106996
- author
- Bentzer, Peter LU ; Holbeck, Staffan LU and Grände, Per-Olof LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Epoprostenol : antagonists & inhibitors : metabolism, Male, Microcirculation : metabolism, Muscle Skeletal : cytology : metabolism, Receptors Endothelin metabolism, Support Non-U.S. Gov't, Tranylcypromine : pharmacology, Time Factors, Endothelin-1 : biosynthesis, Dose-Response Relationship Drug, Cats, Capillary Permeability, Arteries : metabolism, Capillaries : metabolism, Animal
- in
- Microvascular Research
- volume
- 63
- issue
- 1
- pages
- 50 - 60
- publisher
- Academic Press
- external identifiers
-
- wos:000173196800006
- pmid:11749072
- scopus:0036351269
- pmid:11749072
- ISSN
- 1095-9319
- DOI
- 10.1006/mvre.2001.2365
- language
- English
- LU publication?
- yes
- id
- 0915a05f-ddaf-41ee-ab49-3b9df7aeca29 (old id 106996)
- alternative location
- http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11749072&dopt=Abstract
- date added to LUP
- 2016-04-01 12:20:51
- date last changed
- 2022-02-26 05:49:45
@article{0915a05f-ddaf-41ee-ab49-3b9df7aeca29, abstract = {{The aim of the study was to analyze effects of various plasma concentrations of the vasoconstrictor endothelin-1 on microvascular fluid permeability and on transcapillary fluid exchange. We also analyzed whether the permeability-reducing substance prostacyclin is involved in the permeability effects of endothelin-1, as prostacylin is suggested to be released via ET(B) receptor stimulation. The study was performed on an autoperfused cat calf muscle preparation, and a capillary filtration coefficient (CFC) technique was used to estimate variations in microvascular fluid permeability (conductivity). Intraarterial infusion of endothelin-1 in low doses (5 and 10 ng/min/100 g muscle) caused transcapillary absorption, whereas higher doses (20-40 ng/min/100 g) induced filtration despite further vasoconstriction. Low-dose endothelin-1 had no significant effect on CFC, while CFC was reduced to at most 55% of baseline at higher doses (P < 0.01). Simultaneous local intraarterial infusion of the prostacyclin synthesis inhibitor tranylcypromine restored CFC to 114% of baseline (P < 0.01) and further increased vascular resistance. A low, nonvasodilator dose of prostacyclin given intravenously counteracted the tranylcypromine effect on CFC. The decreased CFC induced by a high dose of endothelin-1 was counteracted by the ET(B) receptor antagonist BQ-788 with no change in vascular resistance (P < 0.05). We conclude that the decreased CFC following high doses of endothelin-1 can be attributed to a decrease in microvascular hydraulic conductivity, mediated by secondary release of prostacylin via stimulation of the ET(B) receptor. Endothelin-1 may induce edema through postcapillary vasoconstriction. (c)2001 Elsevier Science.}}, author = {{Bentzer, Peter and Holbeck, Staffan and Grände, Per-Olof}}, issn = {{1095-9319}}, keywords = {{Epoprostenol : antagonists & inhibitors : metabolism; Male; Microcirculation : metabolism; Muscle Skeletal : cytology : metabolism; Receptors Endothelin metabolism; Support Non-U.S. Gov't; Tranylcypromine : pharmacology; Time Factors; Endothelin-1 : biosynthesis; Dose-Response Relationship Drug; Cats; Capillary Permeability; Arteries : metabolism; Capillaries : metabolism; Animal}}, language = {{eng}}, number = {{1}}, pages = {{50--60}}, publisher = {{Academic Press}}, series = {{Microvascular Research}}, title = {{Endothelin-1 Reduces Microvascular Fluid Permeability through Secondary Release of Prostacyclin in Cat Skeletal Muscle.}}, url = {{http://dx.doi.org/10.1006/mvre.2001.2365}}, doi = {{10.1006/mvre.2001.2365}}, volume = {{63}}, year = {{2002}}, }