Reduced oxidative pentose phosphate pathway flux in recombinant xylose-utilizing Saccharomyces cerevisiae strains improves the ethanol yield from xylose.
(2002) In Applied and Environmental Microbiology 68(4). p.1604-1609- Abstract
- In recombinant, xylose-fermenting Saccharomyces cerevisiae, about 30% of the consumed xylose is converted to xylitol. Xylitol production results from a cofactor imbalance, since xylose reductase uses both NADPH and NADH, while xylitol dehydrogenase uses only NAD(+). In this study we increased the ethanol yield and decreased the xylitol yield by lowering the flux through the NADPH-producing pentose phosphate pathway. The pentose phosphate pathway was blocked either by disruption of the GND1 gene, one of the isogenes of 6-phosphogluconate dehydrogenase, or by disruption of the ZWF1 gene, which encodes glucose 6-phosphate dehydrogenase. Decreasing the phosphoglucose isomerase activity by 90% also lowered the pentose phosphate pathway flux.... (More)
- In recombinant, xylose-fermenting Saccharomyces cerevisiae, about 30% of the consumed xylose is converted to xylitol. Xylitol production results from a cofactor imbalance, since xylose reductase uses both NADPH and NADH, while xylitol dehydrogenase uses only NAD(+). In this study we increased the ethanol yield and decreased the xylitol yield by lowering the flux through the NADPH-producing pentose phosphate pathway. The pentose phosphate pathway was blocked either by disruption of the GND1 gene, one of the isogenes of 6-phosphogluconate dehydrogenase, or by disruption of the ZWF1 gene, which encodes glucose 6-phosphate dehydrogenase. Decreasing the phosphoglucose isomerase activity by 90% also lowered the pentose phosphate pathway flux. These modifications all resulted in lower xylitol yield and higher ethanol yield than in the control strains. TMB3255, carrying a disruption of ZWF1, gave the highest ethanol yield (0.41 g g(-1)) and the lowest xylitol yield (0.05 g g(-1)) reported for a xylose-fermenting recombinant S. cerevisiae strain, but also an 84% lower xylose consumption rate. The low xylose fermentation rate is probably due to limited NADPH-mediated xylose reduction. Metabolic flux modeling of TMB3255 confirmed that the NADPH-producing pentose phosphate pathway was blocked and that xylose reduction was mediated only by NADH, leading to a lower rate of xylose consumption. These results indicate that xylitol production is strongly connected to the flux through the oxidative part of the pentose phosphate pathway. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/107215
- author
- Jeppsson, Marie LU ; Johansson, Björn LU ; Hahn-Hägerdal, Bärbel LU and Gorwa-Grauslund, Marie-Francoise LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Ethanol : metabolism, Fungal Proteins : genetics, Fungal Proteins : metabolism, Genetic Engineering : methods, Oxidation-Reduction, Pentosephosphate Pathway : genetics, Pentosephosphate Pathway : physiology, Recombination, Genetic, Saccharomyces cerevisiae : enzymology, Support, Saccharomyces cerevisiae : genetics, Fermentation, Non-U.S. Gov't, Xylose : genetics, Xylose : metabolism
- in
- Applied and Environmental Microbiology
- volume
- 68
- issue
- 4
- pages
- 1604 - 1609
- publisher
- American Society for Microbiology
- external identifiers
-
- wos:000174842200016
- pmid:11916674
- scopus:0036208491
- ISSN
- 0099-2240
- DOI
- 10.1128/AEM.68.4.1604-1609.2002
- language
- English
- LU publication?
- yes
- id
- 48a097dc-4402-4900-99c6-de0c88c30f2f (old id 107215)
- date added to LUP
- 2016-04-01 11:34:26
- date last changed
- 2022-02-03 01:33:42
@article{48a097dc-4402-4900-99c6-de0c88c30f2f,
abstract = {{In recombinant, xylose-fermenting Saccharomyces cerevisiae, about 30% of the consumed xylose is converted to xylitol. Xylitol production results from a cofactor imbalance, since xylose reductase uses both NADPH and NADH, while xylitol dehydrogenase uses only NAD(+). In this study we increased the ethanol yield and decreased the xylitol yield by lowering the flux through the NADPH-producing pentose phosphate pathway. The pentose phosphate pathway was blocked either by disruption of the GND1 gene, one of the isogenes of 6-phosphogluconate dehydrogenase, or by disruption of the ZWF1 gene, which encodes glucose 6-phosphate dehydrogenase. Decreasing the phosphoglucose isomerase activity by 90% also lowered the pentose phosphate pathway flux. These modifications all resulted in lower xylitol yield and higher ethanol yield than in the control strains. TMB3255, carrying a disruption of ZWF1, gave the highest ethanol yield (0.41 g g(-1)) and the lowest xylitol yield (0.05 g g(-1)) reported for a xylose-fermenting recombinant S. cerevisiae strain, but also an 84% lower xylose consumption rate. The low xylose fermentation rate is probably due to limited NADPH-mediated xylose reduction. Metabolic flux modeling of TMB3255 confirmed that the NADPH-producing pentose phosphate pathway was blocked and that xylose reduction was mediated only by NADH, leading to a lower rate of xylose consumption. These results indicate that xylitol production is strongly connected to the flux through the oxidative part of the pentose phosphate pathway.}},
author = {{Jeppsson, Marie and Johansson, Björn and Hahn-Hägerdal, Bärbel and Gorwa-Grauslund, Marie-Francoise}},
issn = {{0099-2240}},
keywords = {{Ethanol : metabolism; Fungal Proteins : genetics; Fungal Proteins : metabolism; Genetic Engineering : methods; Oxidation-Reduction; Pentosephosphate Pathway : genetics; Pentosephosphate Pathway : physiology; Recombination; Genetic; Saccharomyces cerevisiae : enzymology; Support; Saccharomyces cerevisiae : genetics; Fermentation; Non-U.S. Gov't; Xylose : genetics; Xylose : metabolism}},
language = {{eng}},
number = {{4}},
pages = {{1604--1609}},
publisher = {{American Society for Microbiology}},
series = {{Applied and Environmental Microbiology}},
title = {{Reduced oxidative pentose phosphate pathway flux in recombinant xylose-utilizing Saccharomyces cerevisiae strains improves the ethanol yield from xylose.}},
url = {{https://lup.lub.lu.se/search/files/2547167/623588.pdf}},
doi = {{10.1128/AEM.68.4.1604-1609.2002}},
volume = {{68}},
year = {{2002}},
}