Mechanical function of intermediate filaments in arteries of different size examined using desmin deficient mice.
(2002) In Journal of Physiology 540(Pt 3). p.941-949- Abstract
- Protein composition and mechanical function of intermediate filaments were examined in arteries of different sizes using desmin deficient mice (Des-/-) and their wild-type controls (Des+/+). Using SDS-PAGE gels and Western blots we found a gradient in desmin expression in the arterial tree; the desmin content increased from the elastic artery aorta, via the muscular mesenteric artery to the resistance-sized mesenteric microarteries approximately 150 microm in diameter in Des+/+ mice. Mechanical experiments were performed on the aorta, the mesenteric artery and resistance-sized arteries using wire myographs. For aorta and mesenteric artery, no differences in passive or active circumference- stress relations were found between Des-/- and... (More)
- Protein composition and mechanical function of intermediate filaments were examined in arteries of different sizes using desmin deficient mice (Des-/-) and their wild-type controls (Des+/+). Using SDS-PAGE gels and Western blots we found a gradient in desmin expression in the arterial tree; the desmin content increased from the elastic artery aorta, via the muscular mesenteric artery to the resistance-sized mesenteric microarteries approximately 150 microm in diameter in Des+/+ mice. Mechanical experiments were performed on the aorta, the mesenteric artery and resistance-sized arteries using wire myographs. For aorta and mesenteric artery, no differences in passive or active circumference- stress relations were found between Des-/- and Des+/+ mice. In microarteries, both passive and active stress were lower in the Des-/- group. In conclusion, large elastic and muscular arteries contain a relatively low amount of desmin, and the desmin intermediate filaments do not seem to play a major role in the mechanical properties of these larger arterial vessels. In the microarteries, where expression of desmin is high, desmin plays a role in supporting both passive and active tension. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/107958
- author
- Karlsson Wede, Oskar LU ; Löfgren, Mia ; Li, Zhenlin ; Paulin, Denise and Arner, Anders LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Physiology
- volume
- 540
- issue
- Pt 3
- pages
- 941 - 949
- publisher
- The Physiological Society
- external identifiers
-
- wos:000175626300019
- pmid:11986381
- scopus:0036588248
- ISSN
- 1469-7793
- DOI
- 10.1113/jphysiol.2001.014910
- language
- English
- LU publication?
- yes
- id
- 41e8c845-e281-4dec-9879-50cdbbb2bda9 (old id 107958)
- date added to LUP
- 2016-04-01 16:15:02
- date last changed
- 2022-03-14 23:13:43
@article{41e8c845-e281-4dec-9879-50cdbbb2bda9,
abstract = {{Protein composition and mechanical function of intermediate filaments were examined in arteries of different sizes using desmin deficient mice (Des-/-) and their wild-type controls (Des+/+). Using SDS-PAGE gels and Western blots we found a gradient in desmin expression in the arterial tree; the desmin content increased from the elastic artery aorta, via the muscular mesenteric artery to the resistance-sized mesenteric microarteries approximately 150 microm in diameter in Des+/+ mice. Mechanical experiments were performed on the aorta, the mesenteric artery and resistance-sized arteries using wire myographs. For aorta and mesenteric artery, no differences in passive or active circumference- stress relations were found between Des-/- and Des+/+ mice. In microarteries, both passive and active stress were lower in the Des-/- group. In conclusion, large elastic and muscular arteries contain a relatively low amount of desmin, and the desmin intermediate filaments do not seem to play a major role in the mechanical properties of these larger arterial vessels. In the microarteries, where expression of desmin is high, desmin plays a role in supporting both passive and active tension.}},
author = {{Karlsson Wede, Oskar and Löfgren, Mia and Li, Zhenlin and Paulin, Denise and Arner, Anders}},
issn = {{1469-7793}},
language = {{eng}},
number = {{Pt 3}},
pages = {{941--949}},
publisher = {{The Physiological Society}},
series = {{Journal of Physiology}},
title = {{Mechanical function of intermediate filaments in arteries of different size examined using desmin deficient mice.}},
url = {{http://dx.doi.org/10.1113/jphysiol.2001.014910}},
doi = {{10.1113/jphysiol.2001.014910}},
volume = {{540}},
year = {{2002}},
}