Separation and surveys of proteins of Helicobacter pylori.
(2002) In Journal of Chromatography. B 771(1-2). p.251-260- Abstract
- The analysis of Helicobacter pylori proteins is a demanding task for the elucidation of virulence factors, antigens and vaccines, all important for diagnosis, therapy and protection. In the "pre-genomic era" the purification of proteins was mostly performed by using various techniques such as detergent treatment of the bacterial cells, ultra-centrifugation, various chromatographic methods, antibody detection, N-terminal sequence determination and finally cloning and identification of the corresponding gene. In this review, the most representative methods used for purification, separation and identification of H. pylori proteins will be presented as well as some important developments in the "post-genomic era" that have improved the... (More)
- The analysis of Helicobacter pylori proteins is a demanding task for the elucidation of virulence factors, antigens and vaccines, all important for diagnosis, therapy and protection. In the "pre-genomic era" the purification of proteins was mostly performed by using various techniques such as detergent treatment of the bacterial cells, ultra-centrifugation, various chromatographic methods, antibody detection, N-terminal sequence determination and finally cloning and identification of the corresponding gene. In this review, the most representative methods used for purification, separation and identification of H. pylori proteins will be presented as well as some important developments in the "post-genomic era" that have improved the performance of these characterisation techniques. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/108286
- author
- Nilsson, Ingrid LU and Utt, Meeme LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Helicobacter pylori : chemistry, Bacterial, Genome, Two-Dimensional, Gel, Electrophoresis, Bacterial Proteins : isolation & purification, Bacterial Proteins : genetics, Proteome, Helicobacter pylori : genetics, Helicobacter pylori : pathogenicity
- in
- Journal of Chromatography. B
- volume
- 771
- issue
- 1-2
- pages
- 251 - 260
- publisher
- Elsevier
- external identifiers
-
- wos:000175952300015
- pmid:12016003
- scopus:0037023870
- ISSN
- 1873-376X
- DOI
- 10.1016/S1570-0232(02)00113-7
- language
- English
- LU publication?
- yes
- id
- af09504f-98d8-4fc2-b733-5ad2c30ff052 (old id 108286)
- alternative location
- http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12016003&dopt=Abstract
- date added to LUP
- 2016-04-01 12:29:40
- date last changed
- 2022-01-27 05:48:47
@article{af09504f-98d8-4fc2-b733-5ad2c30ff052, abstract = {{The analysis of Helicobacter pylori proteins is a demanding task for the elucidation of virulence factors, antigens and vaccines, all important for diagnosis, therapy and protection. In the "pre-genomic era" the purification of proteins was mostly performed by using various techniques such as detergent treatment of the bacterial cells, ultra-centrifugation, various chromatographic methods, antibody detection, N-terminal sequence determination and finally cloning and identification of the corresponding gene. In this review, the most representative methods used for purification, separation and identification of H. pylori proteins will be presented as well as some important developments in the "post-genomic era" that have improved the performance of these characterisation techniques.}}, author = {{Nilsson, Ingrid and Utt, Meeme}}, issn = {{1873-376X}}, keywords = {{Helicobacter pylori : chemistry; Bacterial; Genome; Two-Dimensional; Gel; Electrophoresis; Bacterial Proteins : isolation & purification; Bacterial Proteins : genetics; Proteome; Helicobacter pylori : genetics; Helicobacter pylori : pathogenicity}}, language = {{eng}}, number = {{1-2}}, pages = {{251--260}}, publisher = {{Elsevier}}, series = {{Journal of Chromatography. B}}, title = {{Separation and surveys of proteins of Helicobacter pylori.}}, url = {{http://dx.doi.org/10.1016/S1570-0232(02)00113-7}}, doi = {{10.1016/S1570-0232(02)00113-7}}, volume = {{771}}, year = {{2002}}, }