Immunogenic proteins of Helicobacter pullorum, Helicobacter bilis and Helicobacter hepaticus identified by two-dimensional gel electrophoresis and immunoblotting.
(2002) In Proteomics 2(6). p.775-783- Abstract
- The ecological niches occupied by various species of Helicobacter are not yet known and the full spectrum of diseases associated with Helicobacter infections are not yet defined. Since these fastidious microaerofilic bacteria require special growth conditions new and improved molecular and serologic diagnostic methods have been developed to increase our understanding of their pathogenesis and virulence characteristics. Immunogenic cell surface proteins of Helicobacter pullorum, Helicobacter bilis, and Helicobacter hepaticus were characterised by proteomic techniques using two-dimensional electrophoresis and immunoblotting with antisera from immunised rabbits. Cross-reactivity between the three Helicobacter species were analysed after a... (More)
- The ecological niches occupied by various species of Helicobacter are not yet known and the full spectrum of diseases associated with Helicobacter infections are not yet defined. Since these fastidious microaerofilic bacteria require special growth conditions new and improved molecular and serologic diagnostic methods have been developed to increase our understanding of their pathogenesis and virulence characteristics. Immunogenic cell surface proteins of Helicobacter pullorum, Helicobacter bilis, and Helicobacter hepaticus were characterised by proteomic techniques using two-dimensional electrophoresis and immunoblotting with antisera from immunised rabbits. Cross-reactivity between the three Helicobacter species were analysed after a four-step cross-absorption experiment. For H. pullorum, H. bilis and H. hepaticus 21, 13 and 27 specific immunogenic proteins, respectively, were identified. These proteins could be of important sero-diagnostic value for analyses of sera from humans, laboratory animals and for the veterinarian field. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/109206
- author
- Kornilovska, Iryna ; Nilsson, Ingrid LU ; Utt, Meeme LU ; Ljungh, Åsa LU and Wadström, Torkel LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Proteomics
- volume
- 2
- issue
- 6
- pages
- 775 - 783
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:12112861
- wos:000176501900023
- scopus:0035987513
- ISSN
- 1615-9861
- DOI
- 10.1002/1615-9861(200206)2:6<775::AID-PROT775>3.0.CO;2-R
- language
- English
- LU publication?
- yes
- id
- aae84f6a-cb4c-41c5-8fca-0b36ae9d94b5 (old id 109206)
- alternative location
- http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12112861&dopt=Abstract
- date added to LUP
- 2016-04-01 12:16:32
- date last changed
- 2022-01-27 01:23:00
@article{aae84f6a-cb4c-41c5-8fca-0b36ae9d94b5,
abstract = {{The ecological niches occupied by various species of Helicobacter are not yet known and the full spectrum of diseases associated with Helicobacter infections are not yet defined. Since these fastidious microaerofilic bacteria require special growth conditions new and improved molecular and serologic diagnostic methods have been developed to increase our understanding of their pathogenesis and virulence characteristics. Immunogenic cell surface proteins of Helicobacter pullorum, Helicobacter bilis, and Helicobacter hepaticus were characterised by proteomic techniques using two-dimensional electrophoresis and immunoblotting with antisera from immunised rabbits. Cross-reactivity between the three Helicobacter species were analysed after a four-step cross-absorption experiment. For H. pullorum, H. bilis and H. hepaticus 21, 13 and 27 specific immunogenic proteins, respectively, were identified. These proteins could be of important sero-diagnostic value for analyses of sera from humans, laboratory animals and for the veterinarian field.}},
author = {{Kornilovska, Iryna and Nilsson, Ingrid and Utt, Meeme and Ljungh, Åsa and Wadström, Torkel}},
issn = {{1615-9861}},
language = {{eng}},
number = {{6}},
pages = {{775--783}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Proteomics}},
title = {{Immunogenic proteins of Helicobacter pullorum, Helicobacter bilis and Helicobacter hepaticus identified by two-dimensional gel electrophoresis and immunoblotting.}},
url = {{http://dx.doi.org/10.1002/1615-9861(200206)2:6<775::AID-PROT775>3.0.CO;2-R}},
doi = {{10.1002/1615-9861(200206)2:6<775::AID-PROT775>3.0.CO;2-R}},
volume = {{2}},
year = {{2002}},
}