Early B-cell factor (o/e-1) is a promoter of adipogenesis and involved in control of genes important for terminal adipocyte differentiation.
(2002) In Molecular and Cellular Biology 22(22). p.8015-8025- Abstract
- Olf-1/early B-cell factor (O/E-1) is a transcription factor important for B-lymphocyte and neuronal gene regulation. Here we report that all three known O/E genes (O/E-1, -2, and -3) are expressed in mouse adipose tissue and are upregulated during adipocyte differentiation. Forced expression of O/E-1 in either the preadipocyte cell line 3T3-L1 or mouse embryonic fibroblasts augmented adipogenesis, and constitutive expression of O/E-1 in uncommitted NIH 3T3 fibroblasts led to initiation of adipocyte differentiation. Furthermore, a dominant negative form of O/E-1 partially suppressed 3T3-L1 adipogenesis, indicating that expression from endogenous O/E target genes is required for 3T3-L1 terminal differentiation. Thus, our data point to the... (More)
- Olf-1/early B-cell factor (O/E-1) is a transcription factor important for B-lymphocyte and neuronal gene regulation. Here we report that all three known O/E genes (O/E-1, -2, and -3) are expressed in mouse adipose tissue and are upregulated during adipocyte differentiation. Forced expression of O/E-1 in either the preadipocyte cell line 3T3-L1 or mouse embryonic fibroblasts augmented adipogenesis, and constitutive expression of O/E-1 in uncommitted NIH 3T3 fibroblasts led to initiation of adipocyte differentiation. Furthermore, a dominant negative form of O/E-1 partially suppressed 3T3-L1 adipogenesis, indicating that expression from endogenous O/E target genes is required for 3T3-L1 terminal differentiation. Thus, our data point to the importance of O/E target genes for adipocyte differentiation and suggest a novel role for O/E-1 as an initiator and stimulator of adipogenesis. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/110544
- author
- Åkerblad, Peter ; Lind, Ulrika ; Liberg, David LU ; Bamberg, Krister and Sigvardsson, Mikael LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Molecular and Cellular Biology
- volume
- 22
- issue
- 22
- pages
- 8015 - 8025
- publisher
- American Society for Microbiology
- external identifiers
-
- wos:000178953700026
- pmid:12391167
- scopus:0036839672
- ISSN
- 0270-7306
- DOI
- 10.1128/MCB.22.22.8015-8025.2002
- language
- English
- LU publication?
- yes
- id
- d668653e-cd4c-4abc-8fc0-459195c327a9 (old id 110544)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12391167&dopt=Abstract
- http://mcb.asm.org/cgi/content/full/22/22/8015?view=full&pmid=12391167
- date added to LUP
- 2016-04-01 11:38:02
- date last changed
- 2022-04-20 19:30:25
@article{d668653e-cd4c-4abc-8fc0-459195c327a9, abstract = {{Olf-1/early B-cell factor (O/E-1) is a transcription factor important for B-lymphocyte and neuronal gene regulation. Here we report that all three known O/E genes (O/E-1, -2, and -3) are expressed in mouse adipose tissue and are upregulated during adipocyte differentiation. Forced expression of O/E-1 in either the preadipocyte cell line 3T3-L1 or mouse embryonic fibroblasts augmented adipogenesis, and constitutive expression of O/E-1 in uncommitted NIH 3T3 fibroblasts led to initiation of adipocyte differentiation. Furthermore, a dominant negative form of O/E-1 partially suppressed 3T3-L1 adipogenesis, indicating that expression from endogenous O/E target genes is required for 3T3-L1 terminal differentiation. Thus, our data point to the importance of O/E target genes for adipocyte differentiation and suggest a novel role for O/E-1 as an initiator and stimulator of adipogenesis.}}, author = {{Åkerblad, Peter and Lind, Ulrika and Liberg, David and Bamberg, Krister and Sigvardsson, Mikael}}, issn = {{0270-7306}}, language = {{eng}}, number = {{22}}, pages = {{8015--8025}}, publisher = {{American Society for Microbiology}}, series = {{Molecular and Cellular Biology}}, title = {{Early B-cell factor (o/e-1) is a promoter of adipogenesis and involved in control of genes important for terminal adipocyte differentiation.}}, url = {{https://lup.lub.lu.se/search/files/2570804/623661.pdf}}, doi = {{10.1128/MCB.22.22.8015-8025.2002}}, volume = {{22}}, year = {{2002}}, }