Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Human monoclonal antibodies with different fine-specificity for digoxin derivatives: Cloning of heavy and light chain variable region sequences

Danielsson, L ; Furebring, Christina LU ; Ohlin, Mats LU orcid ; Hultman, L ; Abrahamson, Magnus LU ; Carlsson, Roland LU and Borrebaeck, Carl LU (1991) In Immunology 74(1). p.50-54
Abstract
Human-mouse hybridoma cell lines producing human monoclonal antibodies against the cardiac glycoside digoxin were established after in vitro immunization or direct immortalization of human peripheral blood lymphocytes with digoxin. Three antibodies, designated M06, LH92 and LH 1 14, displayed different patterns of fine specificity against digoxin and several digoxin analogues, as

elucidated by inhibition ELISA. All three monoclonal antibodies had p heavy chains, two of them (M06 and LH 114) had K light chains and one (LH92) A light chains. DNA encoding the variable regions of both heavy and light chains of the three antibodies were amplified from cDNA using the polymerase chain reaction (PCR). The nucleotide sequences of the... (More)
Human-mouse hybridoma cell lines producing human monoclonal antibodies against the cardiac glycoside digoxin were established after in vitro immunization or direct immortalization of human peripheral blood lymphocytes with digoxin. Three antibodies, designated M06, LH92 and LH 1 14, displayed different patterns of fine specificity against digoxin and several digoxin analogues, as

elucidated by inhibition ELISA. All three monoclonal antibodies had p heavy chains, two of them (M06 and LH 114) had K light chains and one (LH92) A light chains. DNA encoding the variable regions of both heavy and light chains of the three antibodies were amplified from cDNA using the polymerase chain reaction (PCR). The nucleotide sequences of the amplified DNA were determined after subcloning of PCR fragments in M13 vectors. The deduced amino acid sequences revealed considerable sequence differences in the complementarity determining regions between the three antibodies. (Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Immunology
volume
74
issue
1
pages
50 - 54
publisher
Wiley-Blackwell
external identifiers
  • scopus:0025741621
ISSN
0019-2805
language
English
LU publication?
yes
id
8401045b-aa38-436e-ab67-de6600c7d6ae (old id 1106117)
alternative location
http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1937573
date added to LUP
2016-04-01 12:28:44
date last changed
2021-05-25 17:02:23
@article{8401045b-aa38-436e-ab67-de6600c7d6ae,
  abstract     = {{Human-mouse hybridoma cell lines producing human monoclonal antibodies against the cardiac glycoside digoxin were established after in vitro immunization or direct immortalization of human peripheral blood lymphocytes with digoxin. Three antibodies, designated M06, LH92 and LH 1 14, displayed different patterns of fine specificity against digoxin and several digoxin analogues, as<br/><br>
elucidated by inhibition ELISA. All three monoclonal antibodies had p heavy chains, two of them (M06 and LH 114) had K light chains and one (LH92) A light chains. DNA encoding the variable regions of both heavy and light chains of the three antibodies were amplified from cDNA using the polymerase chain reaction (PCR). The nucleotide sequences of the amplified DNA were determined after subcloning of PCR fragments in M13 vectors. The deduced amino acid sequences revealed considerable sequence differences in the complementarity determining regions between the three antibodies.}},
  author       = {{Danielsson, L and Furebring, Christina and Ohlin, Mats and Hultman, L and Abrahamson, Magnus and Carlsson, Roland and Borrebaeck, Carl}},
  issn         = {{0019-2805}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{50--54}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Immunology}},
  title        = {{Human monoclonal antibodies with different fine-specificity for digoxin derivatives: Cloning of heavy and light chain variable region sequences}},
  url          = {{http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1937573}},
  volume       = {{74}},
  year         = {{1991}},
}