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Isolation and identification from Salvia officinalis of two diterpenes which inhibit t-butylbicyclophosphoro[35S]thionate binding to chloride channel of rat cerebrocortical membranes in vitro

Rutherford, Diane M ; Nielsen, Mogens P ; Hansen, Susanne K ; Witt, Michael-Robin ; Bergendorff, Ola LU and Sterner, Olov LU (1992) In Neuroscience Letters 135(2). p.224-226
Abstract
Ethanolic extracts from dried leaves of sage (Salvia officinalis) showed inhibition of [35S]tertiary-butylbicyclophosphorothionate ([35S]TBPS) binding to rat brain membranes in vitro. This ligand is considered to bind to the chloride channel of the GABA/benzodiazepine receptor complex in brain tissue. Substances having inhibitory activity were purified and their chemical structure identified as the diterpenes carnosic acid and carnosol (IC50 values of 33 +/- 3 microM and 57 +/- 4 microM, respectively). The two compounds did not affect binding of the ligands [3H]muscimol and [3H]diazepam to the GABA/benzodiazepine complex in vitro. Saturation experiments of [35S]TBPS binding indicated that carnosic acid decreases the binding affinity.
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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
γ-Aminobutyric acid, Benzodiazepine receptor, [35S]TBPS binding, Salvia officinalis, Diterpenoid, Carnosol, Carnosic acid
in
Neuroscience Letters
volume
135
issue
2
pages
224 - 226
publisher
Elsevier
external identifiers
  • pmid:1378214
  • scopus:0026613007
ISSN
0304-3940
DOI
10.1016/0304-3940(92)90441-9
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Department of Dermatology and Venereology (Lund) (013006000), Organic chemistry (S/LTH) (011001240)
id
8c26a01a-4004-4fb3-bbe1-89bf91155ca7 (old id 1106616)
date added to LUP
2016-04-01 12:26:46
date last changed
2021-05-23 04:36:06
@article{8c26a01a-4004-4fb3-bbe1-89bf91155ca7,
  abstract     = {{Ethanolic extracts from dried leaves of sage (Salvia officinalis) showed inhibition of [35S]tertiary-butylbicyclophosphorothionate ([35S]TBPS) binding to rat brain membranes in vitro. This ligand is considered to bind to the chloride channel of the GABA/benzodiazepine receptor complex in brain tissue. Substances having inhibitory activity were purified and their chemical structure identified as the diterpenes carnosic acid and carnosol (IC50 values of 33 +/- 3 microM and 57 +/- 4 microM, respectively). The two compounds did not affect binding of the ligands [3H]muscimol and [3H]diazepam to the GABA/benzodiazepine complex in vitro. Saturation experiments of [35S]TBPS binding indicated that carnosic acid decreases the binding affinity.}},
  author       = {{Rutherford, Diane M and Nielsen, Mogens P and Hansen, Susanne K and Witt, Michael-Robin and Bergendorff, Ola and Sterner, Olov}},
  issn         = {{0304-3940}},
  keywords     = {{γ-Aminobutyric acid; Benzodiazepine receptor; [35S]TBPS binding; Salvia officinalis; Diterpenoid; Carnosol; Carnosic acid}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{224--226}},
  publisher    = {{Elsevier}},
  series       = {{Neuroscience Letters}},
  title        = {{Isolation and identification from Salvia officinalis of two diterpenes which inhibit t-butylbicyclophosphoro[35S]thionate binding to chloride channel of rat cerebrocortical membranes in vitro}},
  url          = {{http://dx.doi.org/10.1016/0304-3940(92)90441-9}},
  doi          = {{10.1016/0304-3940(92)90441-9}},
  volume       = {{135}},
  year         = {{1992}},
}