Electron microscopy of native cartilage oligomeric matrix protein purified from the Swarm rat chondrosarcoma reveals a five-armed structure
(1992) In Journal of Biological Chemistry 267(9). p.6137-6141- Abstract
- Cartilage oligomeric matrix protein was isolated in the native state from the Swarm rat chondrosarcoma. A crucial step was its selective extraction with EDTA-containing buffer. The purified protein was subjected to electron microscopy using rotary shadowing and negative stain. The images allowed the construction of a structural model. The bouquet-like protein consists of five 28-nm-long arms containing a peripheral globular domain, a flexible strand, and a central assembly domain, where the five arms meet in a cylindrical structure.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1106697
- author
- Mörgelin, Matthias LU ; Heinegård, Dick LU ; Engel, Jürgen and Paulsson, Mats
- organization
- publishing date
- 1992
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Biological Chemistry
- volume
- 267
- issue
- 9
- pages
- 6137 - 6141
- publisher
- American Society for Biochemistry and Molecular Biology
- external identifiers
-
- pmid:1556122
- scopus:0026703257
- ISSN
- 1083-351X
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Connective Tissue Biology (013230151), Division of Infection Medicine (BMC) (013024020)
- id
- 67f156bb-ecc6-4f9c-89ac-ecd0c651d199 (old id 1106697)
- alternative location
- http://www.jbc.org/cgi/reprint/267/9/6137
- date added to LUP
- 2016-04-01 11:45:55
- date last changed
- 2021-03-14 05:08:16
@article{67f156bb-ecc6-4f9c-89ac-ecd0c651d199, abstract = {{Cartilage oligomeric matrix protein was isolated in the native state from the Swarm rat chondrosarcoma. A crucial step was its selective extraction with EDTA-containing buffer. The purified protein was subjected to electron microscopy using rotary shadowing and negative stain. The images allowed the construction of a structural model. The bouquet-like protein consists of five 28-nm-long arms containing a peripheral globular domain, a flexible strand, and a central assembly domain, where the five arms meet in a cylindrical structure.}}, author = {{Mörgelin, Matthias and Heinegård, Dick and Engel, Jürgen and Paulsson, Mats}}, issn = {{1083-351X}}, language = {{eng}}, number = {{9}}, pages = {{6137--6141}}, publisher = {{American Society for Biochemistry and Molecular Biology}}, series = {{Journal of Biological Chemistry}}, title = {{Electron microscopy of native cartilage oligomeric matrix protein purified from the Swarm rat chondrosarcoma reveals a five-armed structure}}, url = {{http://www.jbc.org/cgi/reprint/267/9/6137}}, volume = {{267}}, year = {{1992}}, }