Evaluation of ethanol effects on PLC signal transduction pathways using cell lines of neuronal origin
(1993) In Alcohol and alcoholism. Supplement 2. p.295-299- Abstract
- Human neuroblastoma cells SH-SY5Y and neuroblastoma-glioma cells NG 108-15 have been used as models for the elucidation of the effects of ethanol on receptor-mediated phospholipase C activity, c-fos mRNA expression and protein kinase C activity. Cells were exposed to ethanol (0-200 mM) for varying periods up to seven days. Agonist stimulated events were obtained in NG 108-15 cells with bradykinin and in SH-SY5Y cells with carbachol. Chronic ethanol exposure reduced the agonist-stimulated formation of inositol 1,4,5-trisphosphate in NG 108-15 cells and in SH-SY5Y cells. 100 mM ethanol for seven days increased the membrane bound and cytosolic forms of protein kinase C activity in SH-SY5Y cells. Carbachol (1 mM) induced a maximal c-fos mRNA... (More)
- Human neuroblastoma cells SH-SY5Y and neuroblastoma-glioma cells NG 108-15 have been used as models for the elucidation of the effects of ethanol on receptor-mediated phospholipase C activity, c-fos mRNA expression and protein kinase C activity. Cells were exposed to ethanol (0-200 mM) for varying periods up to seven days. Agonist stimulated events were obtained in NG 108-15 cells with bradykinin and in SH-SY5Y cells with carbachol. Chronic ethanol exposure reduced the agonist-stimulated formation of inositol 1,4,5-trisphosphate in NG 108-15 cells and in SH-SY5Y cells. 100 mM ethanol for seven days increased the membrane bound and cytosolic forms of protein kinase C activity in SH-SY5Y cells. Carbachol (1 mM) induced a maximal c-fos mRNA response after 40 minutes in SH-SY5Y cells, an effect that could be mimicked through protein kinase C stimulation by phorbol esters. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1106956
- author
- Alling, Christer LU ; Bergman, O ; Larsson, Christer LU and Simonsson, Per LU
- organization
- publishing date
- 1993
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Alcohol and alcoholism. Supplement
- volume
- 2
- pages
- 295 - 299
- publisher
- Pergamon Press Ltd.
- external identifiers
-
- pmid:7748314
- scopus:0027902710
- ISSN
- 1358-6173
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Division of Clinical Chemistry and Pharmacology (013250300), Tumour Cell Biology (013017530), Clinical Chemistry, Malmö (013016000)
- id
- 4be1e77e-82f3-409d-ab93-9d89a0cf24ce (old id 1106956)
- date added to LUP
- 2016-04-01 16:10:05
- date last changed
- 2021-01-03 07:16:45
@article{4be1e77e-82f3-409d-ab93-9d89a0cf24ce, abstract = {{Human neuroblastoma cells SH-SY5Y and neuroblastoma-glioma cells NG 108-15 have been used as models for the elucidation of the effects of ethanol on receptor-mediated phospholipase C activity, c-fos mRNA expression and protein kinase C activity. Cells were exposed to ethanol (0-200 mM) for varying periods up to seven days. Agonist stimulated events were obtained in NG 108-15 cells with bradykinin and in SH-SY5Y cells with carbachol. Chronic ethanol exposure reduced the agonist-stimulated formation of inositol 1,4,5-trisphosphate in NG 108-15 cells and in SH-SY5Y cells. 100 mM ethanol for seven days increased the membrane bound and cytosolic forms of protein kinase C activity in SH-SY5Y cells. Carbachol (1 mM) induced a maximal c-fos mRNA response after 40 minutes in SH-SY5Y cells, an effect that could be mimicked through protein kinase C stimulation by phorbol esters.}}, author = {{Alling, Christer and Bergman, O and Larsson, Christer and Simonsson, Per}}, issn = {{1358-6173}}, language = {{eng}}, pages = {{295--299}}, publisher = {{Pergamon Press Ltd.}}, series = {{Alcohol and alcoholism. Supplement}}, title = {{Evaluation of ethanol effects on PLC signal transduction pathways using cell lines of neuronal origin}}, volume = {{2}}, year = {{1993}}, }