Human neuropeptide Y Y1 receptor antisense oligodeoxynucleotide specifically inhibits neuropeptide Y-evoked vasoconstriction
(1993) In European Journal of Pharmacology 240(1). p.77-80- Abstract
- This paper describes a new approach for the development of an inhibitor of the contractile responses of neuropeptide Y in human blood vessels by the use of an antisense oligodeoxynucleotide complementary to human neuropeptide Y Y1 receptor mRNA. One micromolar of an antisense 18-base oligodeoxynucleotide (hY1-AS), corresponding to the human Y1 receptor NH2-terminus, was incubated with segments of human subcutaneous arteries and veins for 48 h at 37 degrees C. Control vessels were incubated with the corresponding sense oligodeoxynucleotide (hY1-S) or a 3-base mismatched antisense oligodeoxynucleotide (hY1-MM) or no oligodeoxynucleotide. The contractile response to neuropeptide Y was markedly attenuated in both arteries and veins after... (More)
- This paper describes a new approach for the development of an inhibitor of the contractile responses of neuropeptide Y in human blood vessels by the use of an antisense oligodeoxynucleotide complementary to human neuropeptide Y Y1 receptor mRNA. One micromolar of an antisense 18-base oligodeoxynucleotide (hY1-AS), corresponding to the human Y1 receptor NH2-terminus, was incubated with segments of human subcutaneous arteries and veins for 48 h at 37 degrees C. Control vessels were incubated with the corresponding sense oligodeoxynucleotide (hY1-S) or a 3-base mismatched antisense oligodeoxynucleotide (hY1-MM) or no oligodeoxynucleotide. The contractile response to neuropeptide Y was markedly attenuated in both arteries and veins after treatment with hY1-AS, but was unaffected by hY1-S or hY1-MM. The pD2 values, i.e. the potency of neuropeptide Y, did not differ in hY1-AS treated vessels, suggesting a non-competitive receptor interaction as a result of down-regulation of Y1 receptors. Responses to noradrenaline or high K+ were unaffected by hY1-AS. This study may represent a new and highly specific approach to vascular pharmacology. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1107192
- author
- Erlinge, David LU ; Edvinsson, Lars LU ; Brunkwall, Jan ; Yee, Frances and Wahlestedt, Claes
- organization
- publishing date
- 1993
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Neuropeptide Y, Blood vessel (human), Antisense, Neuropeptide Y Y1 receptor
- in
- European Journal of Pharmacology
- volume
- 240
- issue
- 1
- pages
- 77 - 80
- publisher
- Elsevier
- external identifiers
-
- pmid:8405124
- scopus:0027281604
- ISSN
- 1879-0712
- DOI
- 10.1016/0014-2999(93)90548-V
- language
- English
- LU publication?
- yes
- id
- 505c414d-afca-434f-925c-d78e8a6f8525 (old id 1107192)
- date added to LUP
- 2016-04-01 12:15:51
- date last changed
- 2024-01-08 14:13:29
@article{505c414d-afca-434f-925c-d78e8a6f8525, abstract = {{This paper describes a new approach for the development of an inhibitor of the contractile responses of neuropeptide Y in human blood vessels by the use of an antisense oligodeoxynucleotide complementary to human neuropeptide Y Y1 receptor mRNA. One micromolar of an antisense 18-base oligodeoxynucleotide (hY1-AS), corresponding to the human Y1 receptor NH2-terminus, was incubated with segments of human subcutaneous arteries and veins for 48 h at 37 degrees C. Control vessels were incubated with the corresponding sense oligodeoxynucleotide (hY1-S) or a 3-base mismatched antisense oligodeoxynucleotide (hY1-MM) or no oligodeoxynucleotide. The contractile response to neuropeptide Y was markedly attenuated in both arteries and veins after treatment with hY1-AS, but was unaffected by hY1-S or hY1-MM. The pD2 values, i.e. the potency of neuropeptide Y, did not differ in hY1-AS treated vessels, suggesting a non-competitive receptor interaction as a result of down-regulation of Y1 receptors. Responses to noradrenaline or high K+ were unaffected by hY1-AS. This study may represent a new and highly specific approach to vascular pharmacology.}}, author = {{Erlinge, David and Edvinsson, Lars and Brunkwall, Jan and Yee, Frances and Wahlestedt, Claes}}, issn = {{1879-0712}}, keywords = {{Neuropeptide Y; Blood vessel (human); Antisense; Neuropeptide Y Y1 receptor}}, language = {{eng}}, number = {{1}}, pages = {{77--80}}, publisher = {{Elsevier}}, series = {{European Journal of Pharmacology}}, title = {{Human neuropeptide Y Y1 receptor antisense oligodeoxynucleotide specifically inhibits neuropeptide Y-evoked vasoconstriction}}, url = {{http://dx.doi.org/10.1016/0014-2999(93)90548-V}}, doi = {{10.1016/0014-2999(93)90548-V}}, volume = {{240}}, year = {{1993}}, }