Contractile effects of neuropeptide Y in human subcutaneous resistance arteries are mediated by Y1 receptors
Nilsson, Torun ; Erlinge, David LU
; Cantera, Leonor
and Edvinsson, Lars
LU
(1996)
In Journal of Cardiovascular Pharmacology
28(6).
p.764-768
- Abstract
- The aim of our study was to determine the neuropeptide Y (NPY) receptor subtype responsible for the NPY-induced contraction of human subcutaneous (s.c.) resistance arteries. To elucidate this, we used (a) in vitro studies of NPY agonists: NPY, peptide YY (PYY), and Pro34NPY induced equally strong and equipotent concentration-dependent contractions of human s.c. resistance arteries, whereas NPY13-36 and NPY18-36 had no contractile effects; (b) in vitro studies using the NPY Y1-receptor antagonist, BIBP3226, which in nanomolar concentrations inhibited the contractile effect of NPY, causing a rightward shift of the concentration-response curve. pEC50 for NPY alone, 8.41 +/- 0.21; NPY + BIBP3226, 10 nM, 7.79 +/- 0.21; NPY + BIBP3226, 100 nM,... (More)
- The aim of our study was to determine the neuropeptide Y (NPY) receptor subtype responsible for the NPY-induced contraction of human subcutaneous (s.c.) resistance arteries. To elucidate this, we used (a) in vitro studies of NPY agonists: NPY, peptide YY (PYY), and Pro34NPY induced equally strong and equipotent concentration-dependent contractions of human s.c. resistance arteries, whereas NPY13-36 and NPY18-36 had no contractile effects; (b) in vitro studies using the NPY Y1-receptor antagonist, BIBP3226, which in nanomolar concentrations inhibited the contractile effect of NPY, causing a rightward shift of the concentration-response curve. pEC50 for NPY alone, 8.41 +/- 0.21; NPY + BIBP3226, 10 nM, 7.79 +/- 0.21; NPY + BIBP3226, 100 nM, 7.18 +/- 0.18; NPY + BIBP3226, 1 microM, 6.32 +/- 0.05 (n = 5-8). Schild-plot analysis indicated competitive antagonism: pA2 = 8.53 +/- 0.22 and slope = 0.99 +/- 0.14; (c) with reverse transcriptase-polymerase chain reaction (RT-PCR), we detected messenger RNA (mRNA) encoding the human NPY Y1 receptor and a splice variant of the receptor in human s.c. resistance arteries. On the basis of the agonists' potency order, the antagonistic effect of BIBP3226 on the NPY-induced contraction, and the presence of mRNA encoding the NPY Y1 receptor, we conclude that the NPY-induced contraction of human s.c. resistance arteries is mediated by NPY Y1 receptors. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1110298
- author
- Nilsson, Torun
; Erlinge, David
LU
; Cantera, Leonor
and Edvinsson, Lars
LU
- organization
- publishing date
- 1996
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Resistance arteries, Neuropeptide Y, Human, BIBP3226, Reverse transcriptase, Polymerase chain reaction
- in
- Journal of Cardiovascular Pharmacology
- volume
- 28
- issue
- 6
- pages
- 764 - 768
- publisher
- Lippincott Williams & Wilkins
- external identifiers
-
- pmid:8961073
- scopus:0029850602
- ISSN
- 1533-4023
- language
- English
- LU publication?
- yes
- id
- 4066ea92-18f8-4cbd-8a48-0706fecfd862 (old id 1110298)
- date added to LUP
- 2016-04-01 11:39:56
- date last changed
- 2024-02-23 00:36:27
@article{4066ea92-18f8-4cbd-8a48-0706fecfd862,
abstract = {{The aim of our study was to determine the neuropeptide Y (NPY) receptor subtype responsible for the NPY-induced contraction of human subcutaneous (s.c.) resistance arteries. To elucidate this, we used (a) in vitro studies of NPY agonists: NPY, peptide YY (PYY), and Pro34NPY induced equally strong and equipotent concentration-dependent contractions of human s.c. resistance arteries, whereas NPY13-36 and NPY18-36 had no contractile effects; (b) in vitro studies using the NPY Y1-receptor antagonist, BIBP3226, which in nanomolar concentrations inhibited the contractile effect of NPY, causing a rightward shift of the concentration-response curve. pEC50 for NPY alone, 8.41 +/- 0.21; NPY + BIBP3226, 10 nM, 7.79 +/- 0.21; NPY + BIBP3226, 100 nM, 7.18 +/- 0.18; NPY + BIBP3226, 1 microM, 6.32 +/- 0.05 (n = 5-8). Schild-plot analysis indicated competitive antagonism: pA2 = 8.53 +/- 0.22 and slope = 0.99 +/- 0.14; (c) with reverse transcriptase-polymerase chain reaction (RT-PCR), we detected messenger RNA (mRNA) encoding the human NPY Y1 receptor and a splice variant of the receptor in human s.c. resistance arteries. On the basis of the agonists' potency order, the antagonistic effect of BIBP3226 on the NPY-induced contraction, and the presence of mRNA encoding the NPY Y1 receptor, we conclude that the NPY-induced contraction of human s.c. resistance arteries is mediated by NPY Y1 receptors.}},
author = {{Nilsson, Torun and Erlinge, David and Cantera, Leonor and Edvinsson, Lars}},
issn = {{1533-4023}},
keywords = {{Resistance arteries; Neuropeptide Y; Human; BIBP3226; Reverse transcriptase; Polymerase chain reaction}},
language = {{eng}},
number = {{6}},
pages = {{764--768}},
publisher = {{Lippincott Williams & Wilkins}},
series = {{Journal of Cardiovascular Pharmacology}},
title = {{Contractile effects of neuropeptide Y in human subcutaneous resistance arteries are mediated by Y1 receptors}},
volume = {{28}},
year = {{1996}},
}