Determination of N-methylsuccinimide and 2-hydroxy-N-methylsuccinimide in human urine and plasma
(1997) In Journal of Chromatography. B 704(1-2). p.151-158- Abstract
- A method for determination of N-methylsuccinimide (MSI) and 2-hydroxy-N-methylsuccinimide (2-HMSI) in human urine and of MSI in human plasma was developed. MSI and 2-HMSI are metabolites of the widely used organic solvent N-methyl-2-pyrrolidone (NMP). MSI and 2-HMSI were purified from urine and plasma by C8 solid-phase extraction and analysed by gas chromatography-mass spectrometry in the negative-ion chemical ionisation mode. The intra-day precisions in urine were 2-6% for MSI (50 and 400 ng/ml) and 3-5% for 2-HMSI (1000 and 8000 ng/ml). For MSI in plasma it was 2% (60 and 1200 ng/ml). The between-day precisions in urine were 3-4% for MSI (100 and 1000 ng/ml) and 2-4% for 2-HMSI (10,000 and 18,000 ng/ml) and 3-4% for MSI in plasma (100... (More)
- A method for determination of N-methylsuccinimide (MSI) and 2-hydroxy-N-methylsuccinimide (2-HMSI) in human urine and of MSI in human plasma was developed. MSI and 2-HMSI are metabolites of the widely used organic solvent N-methyl-2-pyrrolidone (NMP). MSI and 2-HMSI were purified from urine and plasma by C8 solid-phase extraction and analysed by gas chromatography-mass spectrometry in the negative-ion chemical ionisation mode. The intra-day precisions in urine were 2-6% for MSI (50 and 400 ng/ml) and 3-5% for 2-HMSI (1000 and 8000 ng/ml). For MSI in plasma it was 2% (60 and 1200 ng/ml). The between-day precisions in urine were 3-4% for MSI (100 and 1000 ng/ml) and 2-4% for 2-HMSI (10,000 and 18,000 ng/ml) and 3-4% for MSI in plasma (100 and 900 ng/ml). The recoveries from urine were 109-117% for MSI (50 and 400 ng/ml) and 81-89% for 2-HMSI (1000 and 8000 ng/ml). The recovery of MSI from plasma was 91-101% (50 and 500 ng/ml). The detection limits for MSI were 3 ng/ml in urine and 1 ng/ml in plasma and that of 2-HMSI in urine was 200 ng/ml. The method is applicable for analysis of urine and plasma samples from workers exposed to NMP. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1111493
- author
- Jönsson, Bo A LU and Åkesson, Bengt LU
- organization
- publishing date
- 1997
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- N-Methylsuccinimide, 2-Hydroxy-N-methylsuccinimide, N-Methyl-2-pyrrolidone
- in
- Journal of Chromatography. B
- volume
- 704
- issue
- 1-2
- pages
- 151 - 158
- publisher
- Elsevier
- external identifiers
-
- pmid:9518144
- scopus:2542508150
- ISSN
- 1387-2273
- DOI
- 10.1016/S0378-4347(97)00452-0
- language
- English
- LU publication?
- yes
- id
- c5dfd62a-6b6a-4b0c-98e7-4dea203dddd4 (old id 1111493)
- date added to LUP
- 2016-04-01 16:44:31
- date last changed
- 2022-01-28 21:50:25
@article{c5dfd62a-6b6a-4b0c-98e7-4dea203dddd4, abstract = {{A method for determination of N-methylsuccinimide (MSI) and 2-hydroxy-N-methylsuccinimide (2-HMSI) in human urine and of MSI in human plasma was developed. MSI and 2-HMSI are metabolites of the widely used organic solvent N-methyl-2-pyrrolidone (NMP). MSI and 2-HMSI were purified from urine and plasma by C8 solid-phase extraction and analysed by gas chromatography-mass spectrometry in the negative-ion chemical ionisation mode. The intra-day precisions in urine were 2-6% for MSI (50 and 400 ng/ml) and 3-5% for 2-HMSI (1000 and 8000 ng/ml). For MSI in plasma it was 2% (60 and 1200 ng/ml). The between-day precisions in urine were 3-4% for MSI (100 and 1000 ng/ml) and 2-4% for 2-HMSI (10,000 and 18,000 ng/ml) and 3-4% for MSI in plasma (100 and 900 ng/ml). The recoveries from urine were 109-117% for MSI (50 and 400 ng/ml) and 81-89% for 2-HMSI (1000 and 8000 ng/ml). The recovery of MSI from plasma was 91-101% (50 and 500 ng/ml). The detection limits for MSI were 3 ng/ml in urine and 1 ng/ml in plasma and that of 2-HMSI in urine was 200 ng/ml. The method is applicable for analysis of urine and plasma samples from workers exposed to NMP.}}, author = {{Jönsson, Bo A and Åkesson, Bengt}}, issn = {{1387-2273}}, keywords = {{N-Methylsuccinimide; 2-Hydroxy-N-methylsuccinimide; N-Methyl-2-pyrrolidone}}, language = {{eng}}, number = {{1-2}}, pages = {{151--158}}, publisher = {{Elsevier}}, series = {{Journal of Chromatography. B}}, title = {{Determination of N-methylsuccinimide and 2-hydroxy-N-methylsuccinimide in human urine and plasma}}, url = {{http://dx.doi.org/10.1016/S0378-4347(97)00452-0}}, doi = {{10.1016/S0378-4347(97)00452-0}}, volume = {{704}}, year = {{1997}}, }