Determination of 5-hydroxy-N-methylpyrrolidone and 2-hydroxy-N-methylsuccinimide in human urine
(1997) In Journal of Chromatography. B 694(2). p.351-357- Abstract
- A method for simultaneous determination of 5-hydroxy-N-methylpyrrolidone and 2-hydroxy-N-methylsuccinimide in urine is described. These compounds are metabolites of N-methyl-2-pyrrolidone, a powerful and widely used organic solvent. 5-Hydroxy-N-methylpyrrolidone and 2-hydroxy-N-methylsuccinimide were purified from urine by adsorption to a C8 solid-phase extraction column and then elution by ethyl acetate-methanol (80:20). After evaporation, the samples were derivatised at 100 degrees C for 1 h by bis(trimethylsilyl)trifluoroacetamide. Ethyl acetate was then added and the samples were analysed by gas chromatography-mass spectrometry in the electron impact mode. The extraction recovery for 5-hydroxy-N-methylpyrrolidone was about 80% while... (More)
- A method for simultaneous determination of 5-hydroxy-N-methylpyrrolidone and 2-hydroxy-N-methylsuccinimide in urine is described. These compounds are metabolites of N-methyl-2-pyrrolidone, a powerful and widely used organic solvent. 5-Hydroxy-N-methylpyrrolidone and 2-hydroxy-N-methylsuccinimide were purified from urine by adsorption to a C8 solid-phase extraction column and then elution by ethyl acetate-methanol (80:20). After evaporation, the samples were derivatised at 100 degrees C for 1 h by bis(trimethylsilyl)trifluoroacetamide. Ethyl acetate was then added and the samples were analysed by gas chromatography-mass spectrometry in the electron impact mode. The extraction recovery for 5-hydroxy-N-methylpyrrolidone was about 80% while that for 2-hydroxy-N-methylsuccinimide was about 30%. The intra-day precision for 5-hydroxy-N-methylpyrrolidone was 2-4% and the between-day precision 4-21% (4 and 60 microg/ml). The intra-day precision for 2-hydroxy-N-methylsuccinimide was 4-8% and the between-day precision 6-7% (2 and 20 microg/ml). The detection limit was 0.2 microg/ml urine for both compounds. The method is applicable for analysis of urine samples from workers exposed to N-methyl-2-pyrrolidone. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1111495
- author
- Jönsson, Bo A LU and Åkesson, Bengt LU
- organization
- publishing date
- 1997
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- 5-Hydroxy-N-methylpyrrolidone, 2-Hydroxy-N-methylsuccininide, N-Methyl-2-pyrrolidone
- in
- Journal of Chromatography. B
- volume
- 694
- issue
- 2
- pages
- 351 - 357
- publisher
- Elsevier
- external identifiers
-
- pmid:9252049
- scopus:0342276128
- ISSN
- 1387-2273
- DOI
- 10.1016/S0378-4347(97)00125-4
- language
- English
- LU publication?
- yes
- id
- fae68cc6-8f08-4b4a-bd57-c812a29fa23c (old id 1111495)
- date added to LUP
- 2016-04-01 15:33:09
- date last changed
- 2022-01-28 05:54:33
@article{fae68cc6-8f08-4b4a-bd57-c812a29fa23c,
abstract = {{A method for simultaneous determination of 5-hydroxy-N-methylpyrrolidone and 2-hydroxy-N-methylsuccinimide in urine is described. These compounds are metabolites of N-methyl-2-pyrrolidone, a powerful and widely used organic solvent. 5-Hydroxy-N-methylpyrrolidone and 2-hydroxy-N-methylsuccinimide were purified from urine by adsorption to a C8 solid-phase extraction column and then elution by ethyl acetate-methanol (80:20). After evaporation, the samples were derivatised at 100 degrees C for 1 h by bis(trimethylsilyl)trifluoroacetamide. Ethyl acetate was then added and the samples were analysed by gas chromatography-mass spectrometry in the electron impact mode. The extraction recovery for 5-hydroxy-N-methylpyrrolidone was about 80% while that for 2-hydroxy-N-methylsuccinimide was about 30%. The intra-day precision for 5-hydroxy-N-methylpyrrolidone was 2-4% and the between-day precision 4-21% (4 and 60 microg/ml). The intra-day precision for 2-hydroxy-N-methylsuccinimide was 4-8% and the between-day precision 6-7% (2 and 20 microg/ml). The detection limit was 0.2 microg/ml urine for both compounds. The method is applicable for analysis of urine samples from workers exposed to N-methyl-2-pyrrolidone.}},
author = {{Jönsson, Bo A and Åkesson, Bengt}},
issn = {{1387-2273}},
keywords = {{5-Hydroxy-N-methylpyrrolidone; 2-Hydroxy-N-methylsuccininide; N-Methyl-2-pyrrolidone}},
language = {{eng}},
number = {{2}},
pages = {{351--357}},
publisher = {{Elsevier}},
series = {{Journal of Chromatography. B}},
title = {{Determination of 5-hydroxy-N-methylpyrrolidone and 2-hydroxy-N-methylsuccinimide in human urine}},
url = {{http://dx.doi.org/10.1016/S0378-4347(97)00125-4}},
doi = {{10.1016/S0378-4347(97)00125-4}},
volume = {{694}},
year = {{1997}},
}