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Fibronectin binding by Propionibacterium acnes

Yu, Jian-Lin ; Månsson, Roland LU ; Flock, Jan-Ingmar and Ljungh, Åsa LU (1997) In Pathogens and Disease 19(3). p.247-253
Abstract
Strains of Propionibacterium acnes, isolated from different kinds of orthopaedic and biomaterial-associated infections and from skin flora were shown to express binding of soluble as well as immobilized fibronectin. Among these 7 strains isolated from orthopaedic infections, 2 from breast prostheses, and 9 skin isolates, 2, 2, and 5 strains respectively bound immobilized fibronectin. The fibronectin binding was sensitive to protease and heat treatment, and was inhibited by a cell surface extract from one of the binding strains. In SDS-PAGE and autoradiography of cell surface extracts, a band corresponding to a MW of about 80 kD reacted with fibronectin and the 150 kD fragment of fibronectin. Binding to fibronectin and the 150 kD fragment... (More)
Strains of Propionibacterium acnes, isolated from different kinds of orthopaedic and biomaterial-associated infections and from skin flora were shown to express binding of soluble as well as immobilized fibronectin. Among these 7 strains isolated from orthopaedic infections, 2 from breast prostheses, and 9 skin isolates, 2, 2, and 5 strains respectively bound immobilized fibronectin. The fibronectin binding was sensitive to protease and heat treatment, and was inhibited by a cell surface extract from one of the binding strains. In SDS-PAGE and autoradiography of cell surface extracts, a band corresponding to a MW of about 80 kD reacted with fibronectin and the 150 kD fragment of fibronectin. Binding to fibronectin and the 150 kD fragment of fibronectin could be inhibited with heparin. We thus present a first Fn binding protein of P. acnes, a surface exposed protein of 80 kD. None of the strains bound soluble collagen, and only one strain expressed weak binding of vitronectin and bone sialoprotein II. (Less)
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Pathogens and Disease
volume
19
issue
3
pages
247 - 253
publisher
Wiley-Blackwell
external identifiers
  • pmid:9453395
  • scopus:0343035560
ISSN
2049-632X
DOI
10.1111/j.1574-695X.1997.tb01094.x
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Division of Medical Microbiology (013250400), Neurology, Malmö (013027010)
id
337be879-3b25-43bc-9f3f-320686406e89 (old id 1111968)
date added to LUP
2016-04-01 12:15:00
date last changed
2022-03-21 01:28:30
@article{337be879-3b25-43bc-9f3f-320686406e89,
  abstract     = {{Strains of Propionibacterium acnes, isolated from different kinds of orthopaedic and biomaterial-associated infections and from skin flora were shown to express binding of soluble as well as immobilized fibronectin. Among these 7 strains isolated from orthopaedic infections, 2 from breast prostheses, and 9 skin isolates, 2, 2, and 5 strains respectively bound immobilized fibronectin. The fibronectin binding was sensitive to protease and heat treatment, and was inhibited by a cell surface extract from one of the binding strains. In SDS-PAGE and autoradiography of cell surface extracts, a band corresponding to a MW of about 80 kD reacted with fibronectin and the 150 kD fragment of fibronectin. Binding to fibronectin and the 150 kD fragment of fibronectin could be inhibited with heparin. We thus present a first Fn binding protein of P. acnes, a surface exposed protein of 80 kD. None of the strains bound soluble collagen, and only one strain expressed weak binding of vitronectin and bone sialoprotein II.}},
  author       = {{Yu, Jian-Lin and Månsson, Roland and Flock, Jan-Ingmar and Ljungh, Åsa}},
  issn         = {{2049-632X}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{247--253}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Pathogens and Disease}},
  title        = {{Fibronectin binding by Propionibacterium acnes}},
  url          = {{http://dx.doi.org/10.1111/j.1574-695X.1997.tb01094.x}},
  doi          = {{10.1111/j.1574-695X.1997.tb01094.x}},
  volume       = {{19}},
  year         = {{1997}},
}