Summary report of the TD-3 workshop: characterization of 83 antibodies against prostate-specific antigen
(1999) In Tumor Biology 20(Suppl. 1). p.1-12- Abstract
- Twelve research groups participated in the ISOBM TD-3 Workshop in which the reactivity and specificity of 83 antibodies against prostate-specific antigen (PSA) were investigated. Using a variety of techniques including cross-inhibition assays, Western blotting, BIAcore, immunoradiometric assays and immunohistochemistry, the antibodies were categorized into six major groups which formed the basis for mapping onto two- and three-dimensional (2-D and 3-D) models of PSA. The overall findings of the TD-3 Workshop are summarized in this report. In agreement with all participating groups, three main antigenic domains were identified: free PSA-specific epitopes located in or close to amino acids 86-91; discontinuous epitopes specific for PSA... (More)
- Twelve research groups participated in the ISOBM TD-3 Workshop in which the reactivity and specificity of 83 antibodies against prostate-specific antigen (PSA) were investigated. Using a variety of techniques including cross-inhibition assays, Western blotting, BIAcore, immunoradiometric assays and immunohistochemistry, the antibodies were categorized into six major groups which formed the basis for mapping onto two- and three-dimensional (2-D and 3-D) models of PSA. The overall findings of the TD-3 Workshop are summarized in this report. In agreement with all participating groups, three main antigenic domains were identified: free PSA-specific epitopes located in or close to amino acids 86-91; discontinuous epitopes specific for PSA without human kallikrein (hK2) cross-reactivity located at or close to amino acids 158-163; and continuous or linear epitopes shared between PSA and hK2 located close to amino acids 3-11. In addition, several minor and partly overlapping domains were also identified. Clearly, the characterization of antibodies from this workshop and the location of their epitopes on the 3-D model of PSA illustrate the importance of selecting appropriate antibody pairs for use in immunoassays. It is hoped that these findings and the epitope nomenclature described in this TD-3 Workshop are used as a standard for future evaluation of anti-PSA antibodies. (Less)
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https://lup.lub.lu.se/record/1115131
- author
- organization
- publishing date
- 1999
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- PSA, Prostate, Antibody, Workshop, ISOBM
- in
- Tumor Biology
- volume
- 20
- issue
- Suppl. 1
- pages
- 1 - 12
- publisher
- Springer
- external identifiers
-
- pmid:10628402
- scopus:0032726502
- ISSN
- 1423-0380
- DOI
- 10.1159/000056523
- language
- English
- LU publication?
- yes
- id
- dd52b522-f26f-4f81-a0d7-cf93c0ba2804 (old id 1115131)
- date added to LUP
- 2016-04-01 16:39:11
- date last changed
- 2022-01-28 21:12:13
@article{dd52b522-f26f-4f81-a0d7-cf93c0ba2804, abstract = {{Twelve research groups participated in the ISOBM TD-3 Workshop in which the reactivity and specificity of 83 antibodies against prostate-specific antigen (PSA) were investigated. Using a variety of techniques including cross-inhibition assays, Western blotting, BIAcore, immunoradiometric assays and immunohistochemistry, the antibodies were categorized into six major groups which formed the basis for mapping onto two- and three-dimensional (2-D and 3-D) models of PSA. The overall findings of the TD-3 Workshop are summarized in this report. In agreement with all participating groups, three main antigenic domains were identified: free PSA-specific epitopes located in or close to amino acids 86-91; discontinuous epitopes specific for PSA without human kallikrein (hK2) cross-reactivity located at or close to amino acids 158-163; and continuous or linear epitopes shared between PSA and hK2 located close to amino acids 3-11. In addition, several minor and partly overlapping domains were also identified. Clearly, the characterization of antibodies from this workshop and the location of their epitopes on the 3-D model of PSA illustrate the importance of selecting appropriate antibody pairs for use in immunoassays. It is hoped that these findings and the epitope nomenclature described in this TD-3 Workshop are used as a standard for future evaluation of anti-PSA antibodies.}}, author = {{Stenman, U H and Paus, E and Allard, W J and Andersson, I and Andres, C and Barnett, T R and Becker, Charlotte and Belenky, A and Bellanger, L and Pellegrino, C M and Bormer, O P and Davis, G and Dowell, B and Grauer, L S and Jette, D C and Karlsson, B and Kreutz, F T and van der Kwast, T M and Lauren, L and Leinimaa, M and Leinonen, J and Lilja, Hans and Linton, H J and Nap, M and Nilsson, O and Ng, P C and Nustad, K and Peter, A and Pettersson, K and Piironen, T and Rapp, J and Rittenhouse, H G and Rye, P D and Seguin, P and Slota, J and Sokoloff, R L and Suresh, M R and Very, D L and Wang, T J and Wigheden, Ingrid and Wolfert, R L and Yeung, K K and Zhang, W-M and Zhou, Z and Hilgers, J}}, issn = {{1423-0380}}, keywords = {{PSA; Prostate; Antibody; Workshop; ISOBM}}, language = {{eng}}, number = {{Suppl. 1}}, pages = {{1--12}}, publisher = {{Springer}}, series = {{Tumor Biology}}, title = {{Summary report of the TD-3 workshop: characterization of 83 antibodies against prostate-specific antigen}}, url = {{http://dx.doi.org/10.1159/000056523}}, doi = {{10.1159/000056523}}, volume = {{20}}, year = {{1999}}, }