Mouse ten-m/Odz is a new family of dimeric type II transmembrane proteins expressed in many tissues
(1999) In Journal of Cell Biology 145(3). p.563-577- Abstract
- The Drosophila gene ten-m/odz is the only pair rule gene identified to date which is not a transcription factor. In an attempt to analyze the structure and the function of ten-m/odz in mouse, we isolated four murine ten-m cDNAs which code for proteins of 2,700-2, 800 amino acids. All four proteins (Ten-m1-4) lack signal peptides at the NH2 terminus, but contain a short hydrophobic domain characteristic of transmembrane proteins, 300-400 amino acids after the NH2 terminus. About 200 amino acids COOH-terminal to this hydrophobic region are eight consecutive EGF-like domains. Cell transfection, biochemical, and electronmicroscopic studies suggest that Ten-m1 is a dimeric type II transmembrane protein. Expression of fusion proteins composed of... (More)
- The Drosophila gene ten-m/odz is the only pair rule gene identified to date which is not a transcription factor. In an attempt to analyze the structure and the function of ten-m/odz in mouse, we isolated four murine ten-m cDNAs which code for proteins of 2,700-2, 800 amino acids. All four proteins (Ten-m1-4) lack signal peptides at the NH2 terminus, but contain a short hydrophobic domain characteristic of transmembrane proteins, 300-400 amino acids after the NH2 terminus. About 200 amino acids COOH-terminal to this hydrophobic region are eight consecutive EGF-like domains. Cell transfection, biochemical, and electronmicroscopic studies suggest that Ten-m1 is a dimeric type II transmembrane protein. Expression of fusion proteins composed of the NH2-terminal and hydrophobic domain of ten-m1 attached to the alkaline phosphatase reporter gene resulted in membrane-associated staining of the alkaline phosphatase. Electronmicroscopic and electrophoretic analysis of a secreted form of the extracellular domain of Ten-m1 showed that Ten-m1 is a disulfide-linked dimer and that the dimerization is mediated by EGF-like modules 2 and 5 which contain an odd number of cysteines. Northern blot and immunohistochemical analyses revealed widespread expression of mouse ten-m genes, with most prominent expression in brain. All four ten-m genes can be expressed in variously spliced mRNA isoforms. The extracellular domain of Ten-m1 fused to an alkaline phosphatase reporter bound to specific regions in many tissues which were partially overlapping with the Ten-m1 immunostaining. Far Western assays and electronmicroscopy demonstrated that Ten-m1 can bind to itself. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1115468
- author
- Oohashi, T ; Zhou, Xiaohong LU ; Feng, Kang LU ; Richter, B ; Mörgelin, Matthias LU ; Perez, Maria Thereza LU ; Su, W D ; Chiquet-Ehrismann, R ; Rauch, Uwe LU and Fässler, Reinhard LU
- organization
- publishing date
- 1999
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- epidermal growth factor, pair rule, ten-m/odz, transmembrane protein
- in
- Journal of Cell Biology
- volume
- 145
- issue
- 3
- pages
- 563 - 577
- publisher
- Rockefeller University Press
- external identifiers
-
- pmid:10225957
- scopus:0033519207
- ISSN
- 0021-9525
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Ophthalmology (Lund) (013043000), Vessel Wall Biology (013212028), Pathology, (Lund) (013030000), Division of Infection Medicine (BMC) (013024020)
- id
- b32dc2f3-926c-417f-8793-bbb058614038 (old id 1115468)
- alternative location
- http://www.jcb.org/cgi/content/full/145/3/563
- date added to LUP
- 2016-04-01 11:34:38
- date last changed
- 2022-04-28 17:05:59
@article{b32dc2f3-926c-417f-8793-bbb058614038, abstract = {{The Drosophila gene ten-m/odz is the only pair rule gene identified to date which is not a transcription factor. In an attempt to analyze the structure and the function of ten-m/odz in mouse, we isolated four murine ten-m cDNAs which code for proteins of 2,700-2, 800 amino acids. All four proteins (Ten-m1-4) lack signal peptides at the NH2 terminus, but contain a short hydrophobic domain characteristic of transmembrane proteins, 300-400 amino acids after the NH2 terminus. About 200 amino acids COOH-terminal to this hydrophobic region are eight consecutive EGF-like domains. Cell transfection, biochemical, and electronmicroscopic studies suggest that Ten-m1 is a dimeric type II transmembrane protein. Expression of fusion proteins composed of the NH2-terminal and hydrophobic domain of ten-m1 attached to the alkaline phosphatase reporter gene resulted in membrane-associated staining of the alkaline phosphatase. Electronmicroscopic and electrophoretic analysis of a secreted form of the extracellular domain of Ten-m1 showed that Ten-m1 is a disulfide-linked dimer and that the dimerization is mediated by EGF-like modules 2 and 5 which contain an odd number of cysteines. Northern blot and immunohistochemical analyses revealed widespread expression of mouse ten-m genes, with most prominent expression in brain. All four ten-m genes can be expressed in variously spliced mRNA isoforms. The extracellular domain of Ten-m1 fused to an alkaline phosphatase reporter bound to specific regions in many tissues which were partially overlapping with the Ten-m1 immunostaining. Far Western assays and electronmicroscopy demonstrated that Ten-m1 can bind to itself.}}, author = {{Oohashi, T and Zhou, Xiaohong and Feng, Kang and Richter, B and Mörgelin, Matthias and Perez, Maria Thereza and Su, W D and Chiquet-Ehrismann, R and Rauch, Uwe and Fässler, Reinhard}}, issn = {{0021-9525}}, keywords = {{epidermal growth factor; pair rule; ten-m/odz; transmembrane protein}}, language = {{eng}}, number = {{3}}, pages = {{563--577}}, publisher = {{Rockefeller University Press}}, series = {{Journal of Cell Biology}}, title = {{Mouse ten-m/Odz is a new family of dimeric type II transmembrane proteins expressed in many tissues}}, url = {{http://www.jcb.org/cgi/content/full/145/3/563}}, volume = {{145}}, year = {{1999}}, }