Locus-specific multifluor FISH analysis allows physical characterization of complex chromosome abnormalities in neoplasia
(2000) In Genes, Chromosomes and Cancer 28(3). p.347-352- Abstract
- Novel techniques in molecular cytogenetics have radically improved the ability to characterize genetic changes in neoplastic cells. In parallel, a rapid development in high-throughput genomics has resulted in detailed physical maps of the human genome. Combining these two fields, we have developed a method for the simultaneous visualization of several physically defined segments along a chromosome. Seven YAC clones and one subtelomeric cosmid clone from chromosome 12 were labeled with unique combinations of four fluors and hybridized to metaphase chromosomes from neoplastic cells. In a uterine leiomyoma and a myxoid liposarcoma with translocations 12;14 and 12;16, the breakpoints in chromosome 12 could be localized to the HMGIC and CHOP... (More)
- Novel techniques in molecular cytogenetics have radically improved the ability to characterize genetic changes in neoplastic cells. In parallel, a rapid development in high-throughput genomics has resulted in detailed physical maps of the human genome. Combining these two fields, we have developed a method for the simultaneous visualization of several physically defined segments along a chromosome. Seven YAC clones and one subtelomeric cosmid clone from chromosome 12 were labeled with unique combinations of four fluors and hybridized to metaphase chromosomes from neoplastic cells. In a uterine leiomyoma and a myxoid liposarcoma with translocations 12;14 and 12;16, the breakpoints in chromosome 12 could be localized to the HMGIC and CHOP regions, respectively. In the other tumors, more complex aberrations were visualized, including two inversions in 12q with a common breakpoint between MDM2 and D12S332 in a pleomorphic adenoma, amplification of MDM2 and CDK4 in ring chromosomes from a malignant fibrous histiocytoma, and amplification of KRAS2 together with other unbalanced rearrangements in two pancreatic adenocarcinomas. Combinatorially labeled single-copy probes may thus simultaneously provide physical localization of breakpoints and an overview of complex structural rearrangements. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1116723
- author
- Gisselsson Nord, David LU ; Mandahl, Nils LU ; Pålsson, Eva LU ; Gorunova, Ludmila LU and Höglund, Mattias LU
- organization
- publishing date
- 2000
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Genes, Chromosomes and Cancer
- volume
- 28
- issue
- 3
- pages
- 347 - 352
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:10862042
- scopus:0034094217
- ISSN
- 1045-2257
- DOI
- 10.1002/1098-2264(200007)28:3<347::AID-GCC14>3.0.CO;2-2
- language
- English
- LU publication?
- yes
- id
- b5da5f00-2754-44de-ad76-d240c37d80fa (old id 1116723)
- date added to LUP
- 2016-04-01 11:58:45
- date last changed
- 2022-01-26 21:00:40
@article{b5da5f00-2754-44de-ad76-d240c37d80fa, abstract = {{Novel techniques in molecular cytogenetics have radically improved the ability to characterize genetic changes in neoplastic cells. In parallel, a rapid development in high-throughput genomics has resulted in detailed physical maps of the human genome. Combining these two fields, we have developed a method for the simultaneous visualization of several physically defined segments along a chromosome. Seven YAC clones and one subtelomeric cosmid clone from chromosome 12 were labeled with unique combinations of four fluors and hybridized to metaphase chromosomes from neoplastic cells. In a uterine leiomyoma and a myxoid liposarcoma with translocations 12;14 and 12;16, the breakpoints in chromosome 12 could be localized to the HMGIC and CHOP regions, respectively. In the other tumors, more complex aberrations were visualized, including two inversions in 12q with a common breakpoint between MDM2 and D12S332 in a pleomorphic adenoma, amplification of MDM2 and CDK4 in ring chromosomes from a malignant fibrous histiocytoma, and amplification of KRAS2 together with other unbalanced rearrangements in two pancreatic adenocarcinomas. Combinatorially labeled single-copy probes may thus simultaneously provide physical localization of breakpoints and an overview of complex structural rearrangements.}}, author = {{Gisselsson Nord, David and Mandahl, Nils and Pålsson, Eva and Gorunova, Ludmila and Höglund, Mattias}}, issn = {{1045-2257}}, language = {{eng}}, number = {{3}}, pages = {{347--352}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Genes, Chromosomes and Cancer}}, title = {{Locus-specific multifluor FISH analysis allows physical characterization of complex chromosome abnormalities in neoplasia}}, url = {{http://dx.doi.org/10.1002/1098-2264(200007)28:3<347::AID-GCC14>3.0.CO;2-2}}, doi = {{10.1002/1098-2264(200007)28:3<347::AID-GCC14>3.0.CO;2-2}}, volume = {{28}}, year = {{2000}}, }