Comparison of some different methods for analysis of thyroid autoantibodies: Importance of thyroglobulin autoantibodies
(2001) In Thyroid 11(3). p.265-269- Abstract
- Blood samples from 141 children and adolescents were used to evaluate differences between commercial kits and radioimmunoassay (RIA) methods for detecting thyroid autoantibodies. Thyroglobulin autoantibodies (Tg-Ab) were analyzed with a hemagglutination kit and a RIA; thyroid peroxidase autoantibodies (TPO-Ab) were measured with a gelagglutination assay and a RIA. The result; of the antibody tests were compared with thyroid function tests (triiodothyronine [T-3] thyroxine [T-4], thyrotropin [TSH]) and with the results of ultrasound of the thyroid in antibody-positive patients. The correlation of antibody levels between the two methods was higher for TPO-Ab than for Tg-Ab. Moderate to high levels of TPO-Ab correlated to elevated TSH levels.... (More)
- Blood samples from 141 children and adolescents were used to evaluate differences between commercial kits and radioimmunoassay (RIA) methods for detecting thyroid autoantibodies. Thyroglobulin autoantibodies (Tg-Ab) were analyzed with a hemagglutination kit and a RIA; thyroid peroxidase autoantibodies (TPO-Ab) were measured with a gelagglutination assay and a RIA. The result; of the antibody tests were compared with thyroid function tests (triiodothyronine [T-3] thyroxine [T-4], thyrotropin [TSH]) and with the results of ultrasound of the thyroid in antibody-positive patients. The correlation of antibody levels between the two methods was higher for TPO-Ab than for Tg-Ab. Moderate to high levels of TPO-Ab correlated to elevated TSH levels. Auto immune thyroiditis (AIT) was found in 6 of the 141 children. The RIA-based thyroglobulin assay was the only test that identified autoantibodies in all 6 cases. in contrast, the hemagglutination kit thyroglobulin assay failed to identify 4 of the 6 AIT cases. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1119312
- author
- Lindberg, Bengt LU ; Svensson, Johan LU ; Ericsson, Ulla-Britt ; Nilsson, Paul ; Svenonius, Eivor and Ivarsson, Sten LU
- organization
- publishing date
- 2001
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Thyroid
- volume
- 11
- issue
- 3
- pages
- 265 - 269
- publisher
- Mary Ann Liebert, Inc.
- external identifiers
-
- wos:000168143700010
- scopus:0035050878
- ISSN
- 1557-9077
- DOI
- 10.1089/105072501750159697
- language
- English
- LU publication?
- yes
- id
- 028f47a8-7116-4e48-b02d-ef24761af957 (old id 1119312)
- date added to LUP
- 2016-04-01 16:08:38
- date last changed
- 2022-01-28 17:33:44
@article{028f47a8-7116-4e48-b02d-ef24761af957, abstract = {{Blood samples from 141 children and adolescents were used to evaluate differences between commercial kits and radioimmunoassay (RIA) methods for detecting thyroid autoantibodies. Thyroglobulin autoantibodies (Tg-Ab) were analyzed with a hemagglutination kit and a RIA; thyroid peroxidase autoantibodies (TPO-Ab) were measured with a gelagglutination assay and a RIA. The result; of the antibody tests were compared with thyroid function tests (triiodothyronine [T-3] thyroxine [T-4], thyrotropin [TSH]) and with the results of ultrasound of the thyroid in antibody-positive patients. The correlation of antibody levels between the two methods was higher for TPO-Ab than for Tg-Ab. Moderate to high levels of TPO-Ab correlated to elevated TSH levels. Auto immune thyroiditis (AIT) was found in 6 of the 141 children. The RIA-based thyroglobulin assay was the only test that identified autoantibodies in all 6 cases. in contrast, the hemagglutination kit thyroglobulin assay failed to identify 4 of the 6 AIT cases.}}, author = {{Lindberg, Bengt and Svensson, Johan and Ericsson, Ulla-Britt and Nilsson, Paul and Svenonius, Eivor and Ivarsson, Sten}}, issn = {{1557-9077}}, language = {{eng}}, number = {{3}}, pages = {{265--269}}, publisher = {{Mary Ann Liebert, Inc.}}, series = {{Thyroid}}, title = {{Comparison of some different methods for analysis of thyroid autoantibodies: Importance of thyroglobulin autoantibodies}}, url = {{http://dx.doi.org/10.1089/105072501750159697}}, doi = {{10.1089/105072501750159697}}, volume = {{11}}, year = {{2001}}, }