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Fatty-acid-displaced transcriptional repressor, a conserved regulator of cytochrome P450 102 transcription in Bacillus species

Gustafsson, Mattias LU ; Palmer, Colin N. ; Wolf, Roland C. and von Wachenfeldt, Claes LU (2001) In Archives of Microbiology 176(6). p.459-464
Abstract
Bacillus subtilis strain 168 encodes two flavocytochromes P450, Cyp102A2 and Cyp102A3. The cyp102A3 gene is preceded by, and organized in an operon with, a gene for a transcriptional regulator, encoded by fatR. The paralogous gene, cyp102A2, is most likely transcribed as a mono-cistronic message. We show that fatR encodes a protein that binds to an operator sequence that is present upstream of its own reading frame, thereby repressing the expression of the fatR-cyp102A3 operon. Unsaturated fatty acids and phytanic acid have the capacity to interact with FatR and to abrogate its binding to the operator sequence.
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cyp102A2, Cyp102A1, FatR, Bacillus subtilis, cytochrome P450, P450BM-3, Cyp102A3
in
Archives of Microbiology
volume
176
issue
6
pages
459 - 464
publisher
Springer Science and Business Media B.V.
external identifiers
  • pmid:11734890
  • scopus:0035210014
ISSN
0302-8933
DOI
10.1007/s002030100350
language
English
LU publication?
yes
id
3723a02c-8010-4eee-91f7-169bb8b06b4e (old id 1121782)
date added to LUP
2016-04-01 16:41:17
date last changed
2025-04-04 14:54:29
@article{3723a02c-8010-4eee-91f7-169bb8b06b4e,
  abstract     = {{Bacillus subtilis strain 168 encodes two flavocytochromes P450, Cyp102A2 and Cyp102A3. The cyp102A3 gene is preceded by, and organized in an operon with, a gene for a transcriptional regulator, encoded by fatR. The paralogous gene, cyp102A2, is most likely transcribed as a mono-cistronic message. We show that fatR encodes a protein that binds to an operator sequence that is present upstream of its own reading frame, thereby repressing the expression of the fatR-cyp102A3 operon. Unsaturated fatty acids and phytanic acid have the capacity to interact with FatR and to abrogate its binding to the operator sequence.}},
  author       = {{Gustafsson, Mattias and Palmer, Colin N. and Wolf, Roland C. and von Wachenfeldt, Claes}},
  issn         = {{0302-8933}},
  keywords     = {{Cyp102A2; Cyp102A1; FatR; Bacillus subtilis; cytochrome P450; P450BM-3; Cyp102A3}},
  language     = {{eng}},
  number       = {{6}},
  pages        = {{459--464}},
  publisher    = {{Springer Science and Business Media B.V.}},
  series       = {{Archives of Microbiology}},
  title        = {{Fatty-acid-displaced transcriptional repressor, a conserved regulator of cytochrome P450 102 transcription in Bacillus species}},
  url          = {{http://dx.doi.org/10.1007/s002030100350}},
  doi          = {{10.1007/s002030100350}},
  volume       = {{176}},
  year         = {{2001}},
}