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Flow cytometric mapping of the leukotriene B4 receptor, BLT1, in human bone marrow and peripheral blood using specific monoclonal antibodies.

Pettersson, Annika LU ; Richter, Johan LU and Owman, Christer LU (2003) In International Immunopharmacology 3(10-11). p.1467-1475
Abstract
We have previously raised two monoclonal antibodies (7B1, 14F11) recognizing the high-affinity leukotriene B4 receptor, BLT1. They were presently used to determine receptor surface expression in the hematopoietic system. In peripheral blood, BLT1 was primarily recognized in granulocytes, monocytes and, to a lower extent, in certain lymphocytes except the CD4 subpopulation. The expression pattern was similar in bone marrow cells. In vitro differentiation of CD34+ progenitor cells induced BLT1 expression within 7 days, which remained constant up to day 17 when a further increase was measured and maintained up to day 20. BLT1 expression was modified by inflammatory mediators: LPS, TNFalpha, fMLP, as well as LTB4 itself, caused a slight... (More)
We have previously raised two monoclonal antibodies (7B1, 14F11) recognizing the high-affinity leukotriene B4 receptor, BLT1. They were presently used to determine receptor surface expression in the hematopoietic system. In peripheral blood, BLT1 was primarily recognized in granulocytes, monocytes and, to a lower extent, in certain lymphocytes except the CD4 subpopulation. The expression pattern was similar in bone marrow cells. In vitro differentiation of CD34+ progenitor cells induced BLT1 expression within 7 days, which remained constant up to day 17 when a further increase was measured and maintained up to day 20. BLT1 expression was modified by inflammatory mediators: LPS, TNFalpha, fMLP, as well as LTB4 itself, caused a slight down-regulation at 30 min, an effect that was particularly marked with PMA, whereas the effect was least pronounced with IL-8. The antibodies have proved to be useful in an extensive mapping of BLT1 in both peripheral blood and bone marrow and as a tool to elucidate changes in the receptor expression. (Less)
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type
Contribution to journal
publication status
published
subject
in
International Immunopharmacology
volume
3
issue
10-11
pages
1467 - 1475
publisher
Elsevier
external identifiers
  • wos:000185426300012
  • pmid:12946443
  • scopus:0042928298
ISSN
1878-1705
DOI
10.1016/S1567-5769(03)00145-0
language
English
LU publication?
yes
id
25960fbd-a8eb-4019-a032-11a892c9f79d (old id 118045)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12946443&dopt=Abstract
date added to LUP
2016-04-01 12:13:53
date last changed
2026-02-19 10:47:35
@article{25960fbd-a8eb-4019-a032-11a892c9f79d,
  abstract     = {{We have previously raised two monoclonal antibodies (7B1, 14F11) recognizing the high-affinity leukotriene B4 receptor, BLT1. They were presently used to determine receptor surface expression in the hematopoietic system. In peripheral blood, BLT1 was primarily recognized in granulocytes, monocytes and, to a lower extent, in certain lymphocytes except the CD4 subpopulation. The expression pattern was similar in bone marrow cells. In vitro differentiation of CD34+ progenitor cells induced BLT1 expression within 7 days, which remained constant up to day 17 when a further increase was measured and maintained up to day 20. BLT1 expression was modified by inflammatory mediators: LPS, TNFalpha, fMLP, as well as LTB4 itself, caused a slight down-regulation at 30 min, an effect that was particularly marked with PMA, whereas the effect was least pronounced with IL-8. The antibodies have proved to be useful in an extensive mapping of BLT1 in both peripheral blood and bone marrow and as a tool to elucidate changes in the receptor expression.}},
  author       = {{Pettersson, Annika and Richter, Johan and Owman, Christer}},
  issn         = {{1878-1705}},
  language     = {{eng}},
  number       = {{10-11}},
  pages        = {{1467--1475}},
  publisher    = {{Elsevier}},
  series       = {{International Immunopharmacology}},
  title        = {{Flow cytometric mapping of the leukotriene B4 receptor, BLT1, in human bone marrow and peripheral blood using specific monoclonal antibodies.}},
  url          = {{http://dx.doi.org/10.1016/S1567-5769(03)00145-0}},
  doi          = {{10.1016/S1567-5769(03)00145-0}},
  volume       = {{3}},
  year         = {{2003}},
}