Mannan-Degrading Enzymes from Cellulomonas fimi
(1999) In Applied and Environmental Microbiology 65(6). p.2598-2605- Abstract
- The genes man26a and man2A from Cellulomonas fimi encode mannanase 26A (Man26A) and -mannosidase 2A (Man2A), respectively. Mature Man26A is a secreted, modular protein of 951 amino acids, comprising a catalytic module in family 26 of glycosyl hydrolases, an S-layer homology module, and two modules of unknown function. Exposure of Man26A produced by Escherichia coli to C. fimi protease generates active fragments of the enzyme that correspond to polypeptides with mannanase activity produced by C. fimi during growth on mannans, indicating that it may be the only mannanase produced by the organism. A significant fraction of the Man26A produced by C. fimi remains cell associated. Man2A is an intracellular enzyme comprising a catalytic module in... (More)
- The genes man26a and man2A from Cellulomonas fimi encode mannanase 26A (Man26A) and -mannosidase 2A (Man2A), respectively. Mature Man26A is a secreted, modular protein of 951 amino acids, comprising a catalytic module in family 26 of glycosyl hydrolases, an S-layer homology module, and two modules of unknown function. Exposure of Man26A produced by Escherichia coli to C. fimi protease generates active fragments of the enzyme that correspond to polypeptides with mannanase activity produced by C. fimi during growth on mannans, indicating that it may be the only mannanase produced by the organism. A significant fraction of the Man26A produced by C. fimi remains cell associated. Man2A is an intracellular enzyme comprising a catalytic module in a subfamily of family 2 of the glycosyl hydrolases that at present contains only mammalian -mannosidases. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/125413
- author
- Stoll, Dominik ; Stålbrand, Henrik LU and Warren, R Antony
- organization
- publishing date
- 1999
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Applied and Environmental Microbiology
- volume
- 65
- issue
- 6
- pages
- 2598 - 2605
- publisher
- American Society for Microbiology
- external identifiers
-
- scopus:0039180039
- ISSN
- 0099-2240
- language
- English
- LU publication?
- yes
- id
- 693e0c03-48a9-47fc-8534-7a72aeabffa4 (old id 125413)
- alternative location
- http://aem.asm.org/cgi/content/abstract/65/6/2598
- date added to LUP
- 2016-04-01 12:01:50
- date last changed
- 2022-02-18 08:52:22
@article{693e0c03-48a9-47fc-8534-7a72aeabffa4, abstract = {{The genes man26a and man2A from Cellulomonas fimi encode mannanase 26A (Man26A) and -mannosidase 2A (Man2A), respectively. Mature Man26A is a secreted, modular protein of 951 amino acids, comprising a catalytic module in family 26 of glycosyl hydrolases, an S-layer homology module, and two modules of unknown function. Exposure of Man26A produced by Escherichia coli to C. fimi protease generates active fragments of the enzyme that correspond to polypeptides with mannanase activity produced by C. fimi during growth on mannans, indicating that it may be the only mannanase produced by the organism. A significant fraction of the Man26A produced by C. fimi remains cell associated. Man2A is an intracellular enzyme comprising a catalytic module in a subfamily of family 2 of the glycosyl hydrolases that at present contains only mammalian -mannosidases.}}, author = {{Stoll, Dominik and Stålbrand, Henrik and Warren, R Antony}}, issn = {{0099-2240}}, language = {{eng}}, number = {{6}}, pages = {{2598--2605}}, publisher = {{American Society for Microbiology}}, series = {{Applied and Environmental Microbiology}}, title = {{Mannan-Degrading Enzymes from Cellulomonas fimi}}, url = {{http://aem.asm.org/cgi/content/abstract/65/6/2598}}, volume = {{65}}, year = {{1999}}, }