Aqueous two-phase affinity partitioning of biotinylated liposomes using neutral avidin as affinity ligand
(1998) In Journal of Chromatography A 815(2). p.189-195- Abstract
- Biotinylated small unilamellar liposomes were affinity partitioned in an aqueous poly(ethylene glycol)-dextran two-phase system using avidin coupled to dextran as affinity ligand. In the absence of affinity ligand more than 90% of the liposomes partitioned in the poly(ethylene glycol)-rich top phase, whereas in its presence more than 95% partitioned in the dextran-rich bottom phase. For this redistribution to occur 10 mM and above of lithium sulphate, or other appropriate salts, had to be added to the two-phase system. Without added salt the liposomes with complexed avidin-dextran instead partitioned in the top phase. An extended mixing time for the system was required for maximum redistribution. Less than two biotin residues per liposome,... (More)
- Biotinylated small unilamellar liposomes were affinity partitioned in an aqueous poly(ethylene glycol)-dextran two-phase system using avidin coupled to dextran as affinity ligand. In the absence of affinity ligand more than 90% of the liposomes partitioned in the poly(ethylene glycol)-rich top phase, whereas in its presence more than 95% partitioned in the dextran-rich bottom phase. For this redistribution to occur 10 mM and above of lithium sulphate, or other appropriate salts, had to be added to the two-phase system. Without added salt the liposomes with complexed avidin-dextran instead partitioned in the top phase. An extended mixing time for the system was required for maximum redistribution. Less than two biotin residues per liposome, coupled via a C6 spacer arm, was required to redistribute the liposomes to the bottom phase. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/125506
- author
- Ekblad, Lars LU ; Kernbichler, Judith and Jergil, Bengt LU
- organization
- publishing date
- 1998
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Phospholipids
- in
- Journal of Chromatography A
- volume
- 815
- issue
- 2
- pages
- 189 - 195
- publisher
- Elsevier
- external identifiers
-
- scopus:0031857414
- ISSN
- 0021-9673
- DOI
- 10.1016/S0021-9673(98)00433-6
- language
- English
- LU publication?
- yes
- id
- ead80a69-8f5e-443b-9eca-d064b417835f (old id 125506)
- date added to LUP
- 2016-04-01 16:45:09
- date last changed
- 2022-01-28 21:50:32
@article{ead80a69-8f5e-443b-9eca-d064b417835f, abstract = {{Biotinylated small unilamellar liposomes were affinity partitioned in an aqueous poly(ethylene glycol)-dextran two-phase system using avidin coupled to dextran as affinity ligand. In the absence of affinity ligand more than 90% of the liposomes partitioned in the poly(ethylene glycol)-rich top phase, whereas in its presence more than 95% partitioned in the dextran-rich bottom phase. For this redistribution to occur 10 mM and above of lithium sulphate, or other appropriate salts, had to be added to the two-phase system. Without added salt the liposomes with complexed avidin-dextran instead partitioned in the top phase. An extended mixing time for the system was required for maximum redistribution. Less than two biotin residues per liposome, coupled via a C6 spacer arm, was required to redistribute the liposomes to the bottom phase.}}, author = {{Ekblad, Lars and Kernbichler, Judith and Jergil, Bengt}}, issn = {{0021-9673}}, keywords = {{Phospholipids}}, language = {{eng}}, number = {{2}}, pages = {{189--195}}, publisher = {{Elsevier}}, series = {{Journal of Chromatography A}}, title = {{Aqueous two-phase affinity partitioning of biotinylated liposomes using neutral avidin as affinity ligand}}, url = {{http://dx.doi.org/10.1016/S0021-9673(98)00433-6}}, doi = {{10.1016/S0021-9673(98)00433-6}}, volume = {{815}}, year = {{1998}}, }