Positively Regulated Glycerol/G3P-Dependent Bacillus subtilis Gene Expression System Based on Anti-Termination.
(2009) In Journal of Molecular Microbiology and Biotechnology 17. p.61-70- Abstract
- Plasmid pLALA was constructed for glycerol or glycerol-3-phosphate inducible plasmid-borne gene expression in Bacillus subtilis and closely related Gram-positive bacteria. Gene expression using pLALA is based on anti-termination of transcription and involves the B. subtilis GlpP protein that in the presence of glycerol-3-phosphate acts as an anti-terminator protein by binding to the 5'-untranslated region of glpD mRNA. Properties and the usefulness of the system, denoted LALA, were validated by inducible production in B. subtilis strainsof two water-soluble proteins (beta-galactosidase and a protein phospho-tyrosine phosphatase), and one integral membrane protein (heme A synthase). Advantages with LALA is that it is based on positive... (More)
- Plasmid pLALA was constructed for glycerol or glycerol-3-phosphate inducible plasmid-borne gene expression in Bacillus subtilis and closely related Gram-positive bacteria. Gene expression using pLALA is based on anti-termination of transcription and involves the B. subtilis GlpP protein that in the presence of glycerol-3-phosphate acts as an anti-terminator protein by binding to the 5'-untranslated region of glpD mRNA. Properties and the usefulness of the system, denoted LALA, were validated by inducible production in B. subtilis strainsof two water-soluble proteins (beta-galactosidase and a protein phospho-tyrosine phosphatase), and one integral membrane protein (heme A synthase). Advantages with LALA is that it is based on positive control, does not involve a DNA-binding protein, and that glycerol, a cheap and stable compound, can be used as inducer of gene expression. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1262036
- author
- Lewin, Anna LU ; Su, Xiao-Dong and Hederstedt, Lars LU
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Anti-termination, glp, Glycerol inducible, Bacillus subtilis
- in
- Journal of Molecular Microbiology and Biotechnology
- volume
- 17
- pages
- 61 - 70
- publisher
- Karger
- external identifiers
-
- wos:000268373100002
- scopus:68149160868
- pmid:18946204
- ISSN
- 1464-1801
- DOI
- 10.1159/000166210
- language
- English
- LU publication?
- yes
- id
- 49b62a06-b757-4eec-8b8b-37961d8df1c1 (old id 1262036)
- date added to LUP
- 2016-04-01 13:00:46
- date last changed
- 2022-01-27 08:51:32
@article{49b62a06-b757-4eec-8b8b-37961d8df1c1, abstract = {{Plasmid pLALA was constructed for glycerol or glycerol-3-phosphate inducible plasmid-borne gene expression in Bacillus subtilis and closely related Gram-positive bacteria. Gene expression using pLALA is based on anti-termination of transcription and involves the B. subtilis GlpP protein that in the presence of glycerol-3-phosphate acts as an anti-terminator protein by binding to the 5'-untranslated region of glpD mRNA. Properties and the usefulness of the system, denoted LALA, were validated by inducible production in B. subtilis strainsof two water-soluble proteins (beta-galactosidase and a protein phospho-tyrosine phosphatase), and one integral membrane protein (heme A synthase). Advantages with LALA is that it is based on positive control, does not involve a DNA-binding protein, and that glycerol, a cheap and stable compound, can be used as inducer of gene expression.}}, author = {{Lewin, Anna and Su, Xiao-Dong and Hederstedt, Lars}}, issn = {{1464-1801}}, keywords = {{Anti-termination; glp; Glycerol inducible; Bacillus subtilis}}, language = {{eng}}, pages = {{61--70}}, publisher = {{Karger}}, series = {{Journal of Molecular Microbiology and Biotechnology}}, title = {{Positively Regulated Glycerol/G3P-Dependent <em>Bacillus subtilis</em> Gene Expression System Based on Anti-Termination.}}, url = {{http://dx.doi.org/10.1159/000166210}}, doi = {{10.1159/000166210}}, volume = {{17}}, year = {{2009}}, }