Simultaneous detection of ethanol, glucose and glycerol in wines using PQQ - dependent dehydrogenase based biosensors
(2003) In Food Chemistry 82(3). p.481-489- Abstract
- Amperometric biosensors based on corresponding dehydrogenases have been developed for the determination of ethanol, glucose and glycerol. The enzymes have been integrated in redox hydrogels using an Os complex-modified non-conducting polymer employed as the electrochemical mediator and poly(ethyleneglycol)-diglycidyl ether (PEGDGE) as the cross-linking agent. The developed biosensors showed a sensitive response to ethanol, glucose and glycerol within the concentration range 2.5–250, 20–800, and 1–200 M, detection limits of 1.2, 9 and 1 M, and sensitivities of 220, 87 and 32 mA M−1 cm−2, respectively. The ethanol, glucose and glycerol content of several types of wine was determined with these biosensors, and the results were compared with... (More)
- Amperometric biosensors based on corresponding dehydrogenases have been developed for the determination of ethanol, glucose and glycerol. The enzymes have been integrated in redox hydrogels using an Os complex-modified non-conducting polymer employed as the electrochemical mediator and poly(ethyleneglycol)-diglycidyl ether (PEGDGE) as the cross-linking agent. The developed biosensors showed a sensitive response to ethanol, glucose and glycerol within the concentration range 2.5–250, 20–800, and 1–200 M, detection limits of 1.2, 9 and 1 M, and sensitivities of 220, 87 and 32 mA M−1 cm−2, respectively. The ethanol, glucose and glycerol content of several types of wine was determined with these biosensors, and the results were compared with those obtained by spectrophotometric methods. The developed biosensors have been successfully employed for simultaneous determination of all these substrates at the required sensitivity. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/129242
- author
- Niculescu, Mihaela ; Mieliauskiene, Rasa ; Laurinavicius, Valdas and Csöregi, Elisabeth LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Ethanol, Glucose, Glycerol, Biosensor, Wine analysis
- in
- Food Chemistry
- volume
- 82
- issue
- 3
- pages
- 481 - 489
- publisher
- Elsevier
- external identifiers
-
- wos:000184047800021
- scopus:0037671541
- ISSN
- 1873-7072
- DOI
- 10.1016/S0308-8146(03)00118-3
- language
- English
- LU publication?
- yes
- id
- 658e03f7-cada-4ad4-b766-07aa51ddab08 (old id 129242)
- date added to LUP
- 2016-04-01 15:52:13
- date last changed
- 2022-04-07 01:23:02
@article{658e03f7-cada-4ad4-b766-07aa51ddab08, abstract = {{Amperometric biosensors based on corresponding dehydrogenases have been developed for the determination of ethanol, glucose and glycerol. The enzymes have been integrated in redox hydrogels using an Os complex-modified non-conducting polymer employed as the electrochemical mediator and poly(ethyleneglycol)-diglycidyl ether (PEGDGE) as the cross-linking agent. The developed biosensors showed a sensitive response to ethanol, glucose and glycerol within the concentration range 2.5–250, 20–800, and 1–200 M, detection limits of 1.2, 9 and 1 M, and sensitivities of 220, 87 and 32 mA M−1 cm−2, respectively. The ethanol, glucose and glycerol content of several types of wine was determined with these biosensors, and the results were compared with those obtained by spectrophotometric methods. The developed biosensors have been successfully employed for simultaneous determination of all these substrates at the required sensitivity.}}, author = {{Niculescu, Mihaela and Mieliauskiene, Rasa and Laurinavicius, Valdas and Csöregi, Elisabeth}}, issn = {{1873-7072}}, keywords = {{Ethanol; Glucose; Glycerol; Biosensor; Wine analysis}}, language = {{eng}}, number = {{3}}, pages = {{481--489}}, publisher = {{Elsevier}}, series = {{Food Chemistry}}, title = {{Simultaneous detection of ethanol, glucose and glycerol in wines using PQQ - dependent dehydrogenase based biosensors}}, url = {{http://dx.doi.org/10.1016/S0308-8146(03)00118-3}}, doi = {{10.1016/S0308-8146(03)00118-3}}, volume = {{82}}, year = {{2003}}, }