Effects of 17alpha-ethinylestradiol on immune parameters in the lizard Sceloporus occidentalis
(2003) In Environmental Toxicology 18(4). p.211-218- Abstract
- We examined the effect of 17alpha-ethinylestradiol on immunity of the Western fence lizard, Sceloporus occidentalis. Injection of 17alpha-ethinylestradiol resulted in dose-dependent suppression of peripheral blood leukocyte levels as determined by cell counts, whereas total spleen cell levels were decreased only at higher doses of 17alpha-ethinylestradiol. In contrast, spleen cell proliferation was enhanced by 17alpha-ethinylestradiol as measured by reduction of MTT to formazan following a two-way mixed lymphocyte reaction. Antibody responses were unaffected. Effects on peripheral blood leukocyte levels and spleen cell proliferation similar to those observed in response to injection of 17alpha-ethinylestradiol were observed following... (More)
- We examined the effect of 17alpha-ethinylestradiol on immunity of the Western fence lizard, Sceloporus occidentalis. Injection of 17alpha-ethinylestradiol resulted in dose-dependent suppression of peripheral blood leukocyte levels as determined by cell counts, whereas total spleen cell levels were decreased only at higher doses of 17alpha-ethinylestradiol. In contrast, spleen cell proliferation was enhanced by 17alpha-ethinylestradiol as measured by reduction of MTT to formazan following a two-way mixed lymphocyte reaction. Antibody responses were unaffected. Effects on peripheral blood leukocyte levels and spleen cell proliferation similar to those observed in response to injection of 17alpha-ethinylestradiol were observed following injection of a single dose of hydrocortisone. However, injection of lizards with 17alpha-ethinylestradiol did not result in a significant increase in serum cortisol. Results of this study suggest that exposure of Western fence lizards to 17alpha-ethinylestradiol leads to decreased numbers of circulating leukocytes and total spleen cell numbers and the enhancement of spleen cell proliferation in a two-way mixed lymphocyte reaction. These effects probably involve mechanisms other than or in addition to the induction of cortisol release. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1297619
- author
- Burnham, D Kim ; Lackey, Amanda ; Manering, Michelle ; Jaensson Gyllenbäck, Elin LU ; Pearson, Jamie ; Tyler, Daniel O ; Melson, David and Talent, Larry G
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Environmental Toxicology
- volume
- 18
- issue
- 4
- pages
- 211 - 218
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000184632600001
- scopus:0042978779
- ISSN
- 1520-4081
- DOI
- 10.1002/tox.10117
- language
- English
- LU publication?
- no
- id
- 63616822-673a-4fd0-9f99-a235af661940 (old id 1297619)
- date added to LUP
- 2016-04-01 11:41:10
- date last changed
- 2022-01-26 08:44:51
@article{63616822-673a-4fd0-9f99-a235af661940,
abstract = {{We examined the effect of 17alpha-ethinylestradiol on immunity of the Western fence lizard, Sceloporus occidentalis. Injection of 17alpha-ethinylestradiol resulted in dose-dependent suppression of peripheral blood leukocyte levels as determined by cell counts, whereas total spleen cell levels were decreased only at higher doses of 17alpha-ethinylestradiol. In contrast, spleen cell proliferation was enhanced by 17alpha-ethinylestradiol as measured by reduction of MTT to formazan following a two-way mixed lymphocyte reaction. Antibody responses were unaffected. Effects on peripheral blood leukocyte levels and spleen cell proliferation similar to those observed in response to injection of 17alpha-ethinylestradiol were observed following injection of a single dose of hydrocortisone. However, injection of lizards with 17alpha-ethinylestradiol did not result in a significant increase in serum cortisol. Results of this study suggest that exposure of Western fence lizards to 17alpha-ethinylestradiol leads to decreased numbers of circulating leukocytes and total spleen cell numbers and the enhancement of spleen cell proliferation in a two-way mixed lymphocyte reaction. These effects probably involve mechanisms other than or in addition to the induction of cortisol release.}},
author = {{Burnham, D Kim and Lackey, Amanda and Manering, Michelle and Jaensson Gyllenbäck, Elin and Pearson, Jamie and Tyler, Daniel O and Melson, David and Talent, Larry G}},
issn = {{1520-4081}},
language = {{eng}},
number = {{4}},
pages = {{211--218}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Environmental Toxicology}},
title = {{Effects of 17alpha-ethinylestradiol on immune parameters in the lizard Sceloporus occidentalis}},
url = {{http://dx.doi.org/10.1002/tox.10117}},
doi = {{10.1002/tox.10117}},
volume = {{18}},
year = {{2003}},
}