Evaluation of exposure biomarkers from percutaneous absorption of N-methyl-2-pyrrolidone.
(2004) In Scandinavian Journal of Work, Environment and Health 30(4). p.306-312- Abstract
- OBJECTIVES: The aim of this study was to evaluate different biomarkers of exposure to N-methyl-2-pyrrolidone (NMP), a widely used industrial chemical. For this purpose, differences in toxicokinetics between men and women and between pure and water-mixed NMP were evaluated after dermal absorption. METHODS: Six female and six male volunteers (groups 1 and 2) were topically exposed for 6 hours to 300 mg of NMP. An additional group of six male volunteers (group 3) was exposed to 300 mg of NMP in a 50% water solution. Blood and urine were sampled before, during, and up to 9 days after the exposure. Plasma and urine were analyzed using mass spectrometry. RESULTS: For groups 1 and 2, 16% and 18% of the applied dose were recovered in the urine as... (More)
- OBJECTIVES: The aim of this study was to evaluate different biomarkers of exposure to N-methyl-2-pyrrolidone (NMP), a widely used industrial chemical. For this purpose, differences in toxicokinetics between men and women and between pure and water-mixed NMP were evaluated after dermal absorption. METHODS: Six female and six male volunteers (groups 1 and 2) were topically exposed for 6 hours to 300 mg of NMP. An additional group of six male volunteers (group 3) was exposed to 300 mg of NMP in a 50% water solution. Blood and urine were sampled before, during, and up to 9 days after the exposure. Plasma and urine were analyzed using mass spectrometry. RESULTS: For groups 1 and 2, 16% and 18% of the applied dose were recovered in the urine as the sum of NMP and its metabolites. For group 3, 4% was recovered. The maximal concentration of 5-hydroxy-N-methyl-2-pyrrolidone (5-HNMP) was 10, 8.1, and 2.1 micromol/l for groups 1, 2 and 3, respectively, in plasma and 420, 360 and 62 micromol/l in urine adjusted for density. For 2-hydroxy-N-methylsuccinimide (2-HMSI), the maximal concentration was 5.4, 4.5, and 1.3 micromol/l for groups 1, 2 and 3, in plasma, respectively, and 110, 82 and 19 micromol/l in urine adjusted for density. For 5-HNMP there was a difference in time to reach the maximal concentration depending on whether pure NMP or 50% NMP in water was used. No such difference was seen for 2-HMSI. The differences in kinetics between male and female volunteers were small. CONCLUSIONS: Preferably 2-HMSI should be used as the biomarker of exposure to NMP. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/129977
- author
- Åkesson, Bengt LU ; Carnerup, Martin LU and Jönsson, Bo A LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Scandinavian Journal of Work, Environment and Health
- volume
- 30
- issue
- 4
- pages
- 306 - 312
- publisher
- Finnish Institute of Occupational Health
- external identifiers
-
- pmid:15458014
- wos:000223735000007
- scopus:4444262009
- ISSN
- 0355-3140
- language
- English
- LU publication?
- yes
- id
- 1b22e6ea-0c94-4225-bddd-efb5abea22e9 (old id 129977)
- alternative location
- http://www.sjweh.fi/show_abstract.php?abstract_id=799
- date added to LUP
- 2016-04-04 07:07:35
- date last changed
- 2022-04-15 18:37:22
@article{1b22e6ea-0c94-4225-bddd-efb5abea22e9, abstract = {{OBJECTIVES: The aim of this study was to evaluate different biomarkers of exposure to N-methyl-2-pyrrolidone (NMP), a widely used industrial chemical. For this purpose, differences in toxicokinetics between men and women and between pure and water-mixed NMP were evaluated after dermal absorption. METHODS: Six female and six male volunteers (groups 1 and 2) were topically exposed for 6 hours to 300 mg of NMP. An additional group of six male volunteers (group 3) was exposed to 300 mg of NMP in a 50% water solution. Blood and urine were sampled before, during, and up to 9 days after the exposure. Plasma and urine were analyzed using mass spectrometry. RESULTS: For groups 1 and 2, 16% and 18% of the applied dose were recovered in the urine as the sum of NMP and its metabolites. For group 3, 4% was recovered. The maximal concentration of 5-hydroxy-N-methyl-2-pyrrolidone (5-HNMP) was 10, 8.1, and 2.1 micromol/l for groups 1, 2 and 3, respectively, in plasma and 420, 360 and 62 micromol/l in urine adjusted for density. For 2-hydroxy-N-methylsuccinimide (2-HMSI), the maximal concentration was 5.4, 4.5, and 1.3 micromol/l for groups 1, 2 and 3, in plasma, respectively, and 110, 82 and 19 micromol/l in urine adjusted for density. For 5-HNMP there was a difference in time to reach the maximal concentration depending on whether pure NMP or 50% NMP in water was used. No such difference was seen for 2-HMSI. The differences in kinetics between male and female volunteers were small. CONCLUSIONS: Preferably 2-HMSI should be used as the biomarker of exposure to NMP.}}, author = {{Åkesson, Bengt and Carnerup, Martin and Jönsson, Bo A}}, issn = {{0355-3140}}, language = {{eng}}, number = {{4}}, pages = {{306--312}}, publisher = {{Finnish Institute of Occupational Health}}, series = {{Scandinavian Journal of Work, Environment and Health}}, title = {{Evaluation of exposure biomarkers from percutaneous absorption of N-methyl-2-pyrrolidone.}}, url = {{http://www.sjweh.fi/show_abstract.php?abstract_id=799}}, volume = {{30}}, year = {{2004}}, }