Brevican distinctively assembles extracellular components at the large diameter nodes of Ranvier in the CNS
(2009) In Journal of Neurochemistry 108(5). p.1266-1276- Abstract
- Brevican is known to be an abundant extracellular matrix component in the adult brain and a structural constituent of perineuronal nets. We herein show that brevican, tenascin-R (TN-R) and phosphacan are present at the nodes of Ranvier on myelinated axons with a particularly large diameter in the central nervous system. A brevican deficiency resulted in a reorganization of the nodal matrices, which was characterized by the shift of TN-R, and concomitantly phosphacan, from an axonal diameter-dependent association with nodes to an axonal diameter independent association. Supported by the co-immunoprecipitation results, these observations indicate that the presence of TN-R and phosphacan at nodes is normally brevican-dependent, while in the... (More)
- Brevican is known to be an abundant extracellular matrix component in the adult brain and a structural constituent of perineuronal nets. We herein show that brevican, tenascin-R (TN-R) and phosphacan are present at the nodes of Ranvier on myelinated axons with a particularly large diameter in the central nervous system. A brevican deficiency resulted in a reorganization of the nodal matrices, which was characterized by the shift of TN-R, and concomitantly phosphacan, from an axonal diameter-dependent association with nodes to an axonal diameter independent association. Supported by the co-immunoprecipitation results, these observations indicate that the presence of TN-R and phosphacan at nodes is normally brevican-dependent, while in the absence of brevican these molecules can also be recruited by versican V2. The versican V2 and Bral1 distribution was not affected, thus indicating a brevican-independent role of these two molecules for establishing hyaluronan-binding matrices at the nodes. Our results revealed that brevican plays a crucial role in determining the specialization of the hyaluronan-binding nodal matrix assemblies in large diameter nodes. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1311411
- author
- Bekku, Yoko ; Rauch, Uwe LU ; Ninomiya, Yoshifumi and Oohashi, Toshitaka
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- tenascin-R, scaffolding proteins, proteoglycan, node of Ranvier, action potential, extracellular matrix
- in
- Journal of Neurochemistry
- volume
- 108
- issue
- 5
- pages
- 1266 - 1276
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000263044800016
- scopus:59449103926
- pmid:19141078
- ISSN
- 1471-4159
- DOI
- 10.1111/j.1471-4159.2009.05873.x
- language
- English
- LU publication?
- yes
- id
- 6db3cfc1-16d5-4753-81d2-aa3319127a33 (old id 1311411)
- date added to LUP
- 2016-04-01 14:04:19
- date last changed
- 2022-04-22 01:11:53
@article{6db3cfc1-16d5-4753-81d2-aa3319127a33,
abstract = {{Brevican is known to be an abundant extracellular matrix component in the adult brain and a structural constituent of perineuronal nets. We herein show that brevican, tenascin-R (TN-R) and phosphacan are present at the nodes of Ranvier on myelinated axons with a particularly large diameter in the central nervous system. A brevican deficiency resulted in a reorganization of the nodal matrices, which was characterized by the shift of TN-R, and concomitantly phosphacan, from an axonal diameter-dependent association with nodes to an axonal diameter independent association. Supported by the co-immunoprecipitation results, these observations indicate that the presence of TN-R and phosphacan at nodes is normally brevican-dependent, while in the absence of brevican these molecules can also be recruited by versican V2. The versican V2 and Bral1 distribution was not affected, thus indicating a brevican-independent role of these two molecules for establishing hyaluronan-binding matrices at the nodes. Our results revealed that brevican plays a crucial role in determining the specialization of the hyaluronan-binding nodal matrix assemblies in large diameter nodes.}},
author = {{Bekku, Yoko and Rauch, Uwe and Ninomiya, Yoshifumi and Oohashi, Toshitaka}},
issn = {{1471-4159}},
keywords = {{tenascin-R; scaffolding proteins; proteoglycan; node of Ranvier; action potential; extracellular matrix}},
language = {{eng}},
number = {{5}},
pages = {{1266--1276}},
publisher = {{Wiley-Blackwell}},
series = {{Journal of Neurochemistry}},
title = {{Brevican distinctively assembles extracellular components at the large diameter nodes of Ranvier in the CNS}},
url = {{http://dx.doi.org/10.1111/j.1471-4159.2009.05873.x}},
doi = {{10.1111/j.1471-4159.2009.05873.x}},
volume = {{108}},
year = {{2009}},
}