Mass overloading in the flow field-flow fractionation channel studied by the behaviour of the ultra-large wheat protein glutein.
(2003) In Journal of Chromatography A 1011(1-2). p.99-109- Abstract
- Flow field-flow fractionation (FFF) has previously been used in successful fractionation and characterisation of the ultra-large wheat protein glutenin. The many parameters, which may influence the retention behaviour, especially when analysing extremely high-molecular-mass samples such as glutenin, are here reported. Size determination from the sample retention time, using FFF theory, will as a result have a very low accuracy. The need for direct molecular mass determination, such as by light scattering, in combination with FFF, in order to do accurate size measurements of glutenin is pointed out as well as the importance to minimise the overloading.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/132717
- author
- Arfvidsson, Cecilia LU and Wahlund, Karl-Gustav LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Chromatography A
- volume
- 1011
- issue
- 1-2
- pages
- 99 - 109
- publisher
- Elsevier
- external identifiers
-
- wos:000185157500011
- pmid:14518767
- scopus:0042521032
- ISSN
- 0021-9673
- DOI
- 10.1016/S0021-9673(03)01145-2
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- 743ac15a-dd00-4641-992c-fe907bd3785a (old id 132717)
- date added to LUP
- 2016-04-01 15:34:11
- date last changed
- 2022-01-28 06:00:01
@article{743ac15a-dd00-4641-992c-fe907bd3785a, abstract = {{Flow field-flow fractionation (FFF) has previously been used in successful fractionation and characterisation of the ultra-large wheat protein glutenin. The many parameters, which may influence the retention behaviour, especially when analysing extremely high-molecular-mass samples such as glutenin, are here reported. Size determination from the sample retention time, using FFF theory, will as a result have a very low accuracy. The need for direct molecular mass determination, such as by light scattering, in combination with FFF, in order to do accurate size measurements of glutenin is pointed out as well as the importance to minimise the overloading.}}, author = {{Arfvidsson, Cecilia and Wahlund, Karl-Gustav}}, issn = {{0021-9673}}, language = {{eng}}, number = {{1-2}}, pages = {{99--109}}, publisher = {{Elsevier}}, series = {{Journal of Chromatography A}}, title = {{Mass overloading in the flow field-flow fractionation channel studied by the behaviour of the ultra-large wheat protein glutein.}}, url = {{http://dx.doi.org/10.1016/S0021-9673(03)01145-2}}, doi = {{10.1016/S0021-9673(03)01145-2}}, volume = {{1011}}, year = {{2003}}, }