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High capacity xylose transport in Candida intermedia PYCC4715.

Gárdonyi, Márk LU ; Österberg, Måns ; Rodrigues, C ; Spencer-Martins, I and Hahn-Hägerdal, Bärbel LU (2003) In FEMS Yeast Research 3(1). p.45-52
Abstract
Xylose-utilising yeasts were screened to identify strains with high xylose transport capacity. Among the fastest-growing strains in xylose medium, Candida intermedia PYCC 4715 showed the highest xylose transport capacity. Maximal specific growth rate was the same in glucose and xylose media (small mu, Greekmax=0.5 h−1, 30°C). Xylose transport showed biphasic kinetics when cells were grown in either xylose- or glucose-limited culture. The high-affinity xylose/proton symport system (Km=0.2 mM, Vmax=7.5 mmol h−1 g−1) was more repressed by glucose than by xylose. The less specific low-affinity transport system (K=50 mM, Vmax=11 mmol h−1 g−1) appeared to operate through a facilitated-diffusion mechanism and was expressed constitutively.... (More)
Xylose-utilising yeasts were screened to identify strains with high xylose transport capacity. Among the fastest-growing strains in xylose medium, Candida intermedia PYCC 4715 showed the highest xylose transport capacity. Maximal specific growth rate was the same in glucose and xylose media (small mu, Greekmax=0.5 h−1, 30°C). Xylose transport showed biphasic kinetics when cells were grown in either xylose- or glucose-limited culture. The high-affinity xylose/proton symport system (Km=0.2 mM, Vmax=7.5 mmol h−1 g−1) was more repressed by glucose than by xylose. The less specific low-affinity transport system (K=50 mM, Vmax=11 mmol h−1 g−1) appeared to operate through a facilitated-diffusion mechanism and was expressed constitutively. Inhibition experiments showed that glucose is a substrate of both xylose transport systems. (Less)
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
FEMS Yeast Research
volume
3
issue
1
pages
45 - 52
publisher
Oxford University Press
external identifiers
  • wos:000181502500007
  • scopus:0037369565
ISSN
1567-1364
DOI
10.1016/S1567-1356(02)00137-X
language
English
LU publication?
yes
id
8c48c7f9-5e14-4ff0-b45d-825a3560e4ba (old id 132782)
date added to LUP
2016-04-01 11:52:55
date last changed
2022-01-26 19:37:13
@article{8c48c7f9-5e14-4ff0-b45d-825a3560e4ba,
  abstract     = {{Xylose-utilising yeasts were screened to identify strains with high xylose transport capacity. Among the fastest-growing strains in xylose medium, Candida intermedia PYCC 4715 showed the highest xylose transport capacity. Maximal specific growth rate was the same in glucose and xylose media (small mu, Greekmax=0.5 h−1, 30°C). Xylose transport showed biphasic kinetics when cells were grown in either xylose- or glucose-limited culture. The high-affinity xylose/proton symport system (Km=0.2 mM, Vmax=7.5 mmol h−1 g−1) was more repressed by glucose than by xylose. The less specific low-affinity transport system (K=50 mM, Vmax=11 mmol h−1 g−1) appeared to operate through a facilitated-diffusion mechanism and was expressed constitutively. Inhibition experiments showed that glucose is a substrate of both xylose transport systems.}},
  author       = {{Gárdonyi, Márk and Österberg, Måns and Rodrigues, C and Spencer-Martins, I and Hahn-Hägerdal, Bärbel}},
  issn         = {{1567-1364}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{45--52}},
  publisher    = {{Oxford University Press}},
  series       = {{FEMS Yeast Research}},
  title        = {{High capacity xylose transport in Candida intermedia PYCC4715.}},
  url          = {{http://dx.doi.org/10.1016/S1567-1356(02)00137-X}},
  doi          = {{10.1016/S1567-1356(02)00137-X}},
  volume       = {{3}},
  year         = {{2003}},
}