Reduced hippocampal neurogenesis in R6/2 transgenic Huntington's disease mice.
(2005) In Neurobiology of Disease 20(3). p.744-751- Abstract
- We investigated whether cell proliferation and neurogenesis are altered in R6/2 transgenic Huntington's disease mice. Using bromodeoxyuridine (BrdU), we found a progressive decrease in the number of proliferating cells in the dentate gyrus of R6/2 mice. This reduction was detected in pre-symptomatic mice, and by 11.5 weeks, R6/2 mice had 66% fewer newly born cells in the hippocampus. The results were confirmed by immunohistochemistry for the cell cycle markers Ki-67 and proliferating cell nuclear antigen (PCNA). We did not observe changes in cell proliferation in the R6/2 subventricular zone, indicating that the decrease in cell proliferation is specific for the hippocampus. This decrease corresponded to a reduction in actual hippocampal... (More)
- We investigated whether cell proliferation and neurogenesis are altered in R6/2 transgenic Huntington's disease mice. Using bromodeoxyuridine (BrdU), we found a progressive decrease in the number of proliferating cells in the dentate gyrus of R6/2 mice. This reduction was detected in pre-symptomatic mice, and by 11.5 weeks, R6/2 mice had 66% fewer newly born cells in the hippocampus. The results were confirmed by immunohistochemistry for the cell cycle markers Ki-67 and proliferating cell nuclear antigen (PCNA). We did not observe changes in cell proliferation in the R6/2 subventricular zone, indicating that the decrease in cell proliferation is specific for the hippocampus. This decrease corresponded to a reduction in actual hippocampal neurogenesis as assessed by double immunostaining for BrdU and the neuronal marker neuronal nuclei (NeuN) and by immunohistochemistry for the neuroblast marker doublecortin. Reduced hippocampal neurogenesis may be a novel neuropathological feature in R6/2 mice that could be assessed when evaluating potential therapies. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/140069
- author
- Gil, Joana LU ; Mohapel, Paul LU ; Araujo, Ines LU ; Popovic, Natalija LU ; Li, Jia-Yi LU ; Brundin, Patrik LU and Petersén, Åsa LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Cell proliferation, Dentate gyrus, Doublecortin, Huntington's disease, Ki-67, Neurogenesis, R6/2 mice, PCNA, Subventricular zone, BrdU
- in
- Neurobiology of Disease
- volume
- 20
- issue
- 3
- pages
- 744 - 751
- publisher
- Academic Press
- external identifiers
-
- wos:000233739100013
- pmid:15951191
- scopus:27644485740
- pmid:15951191
- ISSN
- 0969-9961
- DOI
- 10.1016/j.nbd.2005.05.006
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Neuronal Survival (013212041), Wallenberg Neuroscience Centre, Lund (0131000110)
- id
- 67b2862a-c1f3-42e7-bd1e-c345b97e43f6 (old id 140069)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15951191&query_hl=14006
- date added to LUP
- 2016-04-01 11:32:58
- date last changed
- 2025-04-04 14:52:40
@article{67b2862a-c1f3-42e7-bd1e-c345b97e43f6, abstract = {{We investigated whether cell proliferation and neurogenesis are altered in R6/2 transgenic Huntington's disease mice. Using bromodeoxyuridine (BrdU), we found a progressive decrease in the number of proliferating cells in the dentate gyrus of R6/2 mice. This reduction was detected in pre-symptomatic mice, and by 11.5 weeks, R6/2 mice had 66% fewer newly born cells in the hippocampus. The results were confirmed by immunohistochemistry for the cell cycle markers Ki-67 and proliferating cell nuclear antigen (PCNA). We did not observe changes in cell proliferation in the R6/2 subventricular zone, indicating that the decrease in cell proliferation is specific for the hippocampus. This decrease corresponded to a reduction in actual hippocampal neurogenesis as assessed by double immunostaining for BrdU and the neuronal marker neuronal nuclei (NeuN) and by immunohistochemistry for the neuroblast marker doublecortin. Reduced hippocampal neurogenesis may be a novel neuropathological feature in R6/2 mice that could be assessed when evaluating potential therapies.}}, author = {{Gil, Joana and Mohapel, Paul and Araujo, Ines and Popovic, Natalija and Li, Jia-Yi and Brundin, Patrik and Petersén, Åsa}}, issn = {{0969-9961}}, keywords = {{Cell proliferation; Dentate gyrus; Doublecortin; Huntington's disease; Ki-67; Neurogenesis; R6/2 mice; PCNA; Subventricular zone; BrdU}}, language = {{eng}}, number = {{3}}, pages = {{744--751}}, publisher = {{Academic Press}}, series = {{Neurobiology of Disease}}, title = {{Reduced hippocampal neurogenesis in R6/2 transgenic Huntington's disease mice.}}, url = {{http://dx.doi.org/10.1016/j.nbd.2005.05.006}}, doi = {{10.1016/j.nbd.2005.05.006}}, volume = {{20}}, year = {{2005}}, }