Arabidopsis RADICAL-INDUCED CELL DEATH1 is involved in UV-B signaling.
Jiang, Lei ; Wang, Yan ; Björn, Lars Olof LU
and Li, Shaoshan
(2009)
In Photochemical and Photobiological Sciences
8.
p.838-846
- Abstract
- The Arabidopsis radical-induced cell death1 (rcd1) mutant is sensitive to ozone fumigation and
apoplastic superoxide, but tolerant to methyl viologen. In the present article, we report that the rcd1
mutant is also tolerant to supplementary UV-B radiation. The rcd1-1 mutant exhibits less accumulation
of TT dimers, increased hypocotyl growth inhibition and higher accumulation of flavonoids under
supplemental UV-B radiation. Moreover, the expression of HY5 (ELONGATED HYPOCOTYL5) is
increased in the mutant after UV-B treatment. Gene expression downstream of UV-B signaling reveals
that COP1 (CONSTITUTIVELY PHOTOMORPHOGENIC1)-regulated genes have an elevated
expression in rcd1-1... (More) - The Arabidopsis radical-induced cell death1 (rcd1) mutant is sensitive to ozone fumigation and
apoplastic superoxide, but tolerant to methyl viologen. In the present article, we report that the rcd1
mutant is also tolerant to supplementary UV-B radiation. The rcd1-1 mutant exhibits less accumulation
of TT dimers, increased hypocotyl growth inhibition and higher accumulation of flavonoids under
supplemental UV-B radiation. Moreover, the expression of HY5 (ELONGATED HYPOCOTYL5) is
increased in the mutant after UV-B treatment. Gene expression downstream of UV-B signaling reveals
that COP1 (CONSTITUTIVELY PHOTOMORPHOGENIC1)-regulated genes have an elevated
expression in rcd1-1 mutant under UV-B radiation, while expression of UVR8 (UV RESISTANCE
LOCUS 8)-regulated and HY5-independent genes are not changed. Interestingly, the expression of
RCD1 genes is not significantly changed by UV-B radiation. Previous study has shown that STO
protein is interacting with RCD1 in vitro. Here, we found the mRNA level of STO (SALT
TOLERANCE) is greatly increased in rcd1-1 mutant after UV-B radiation. However, UV-B-induced
HY5 and CHS expression is partially inhibited in sto mutant. Based on the above results, it is deduced
that the RCD1, working together with STO, is involved in Arabidopsis UV-B signaling. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1419952
- author
- Jiang, Lei
; Wang, Yan
; Björn, Lars Olof
LU
and Li, Shaoshan
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- UV-B, ultraviolet radiation, signaling, Arabidopis, rcd1
- in
- Photochemical and Photobiological Sciences
- volume
- 8
- pages
- 838 - 846
- publisher
- Royal Society of Chemistry
- external identifiers
-
- wos:000266598000013
- scopus:67449151623
- ISSN
- 1474-9092
- DOI
- 10.1039/b901187k
- project
- Photobiology
- language
- English
- LU publication?
- yes
- id
- 5cb6a7fd-4ef2-4551-a72d-8e9edb52a218 (old id 1419952)
- date added to LUP
- 2016-04-01 11:52:15
- date last changed
- 2022-03-13 01:55:43
@article{5cb6a7fd-4ef2-4551-a72d-8e9edb52a218,
abstract = {{The Arabidopsis radical-induced cell death1 (rcd1) mutant is sensitive to ozone fumigation and<br/><br>
apoplastic superoxide, but tolerant to methyl viologen. In the present article, we report that the rcd1<br/><br>
mutant is also tolerant to supplementary UV-B radiation. The rcd1-1 mutant exhibits less accumulation<br/><br>
of TT dimers, increased hypocotyl growth inhibition and higher accumulation of flavonoids under<br/><br>
supplemental UV-B radiation. Moreover, the expression of HY5 (ELONGATED HYPOCOTYL5) is<br/><br>
increased in the mutant after UV-B treatment. Gene expression downstream of UV-B signaling reveals<br/><br>
that COP1 (CONSTITUTIVELY PHOTOMORPHOGENIC1)-regulated genes have an elevated<br/><br>
expression in rcd1-1 mutant under UV-B radiation, while expression of UVR8 (UV RESISTANCE<br/><br>
LOCUS 8)-regulated and HY5-independent genes are not changed. Interestingly, the expression of<br/><br>
RCD1 genes is not significantly changed by UV-B radiation. Previous study has shown that STO<br/><br>
protein is interacting with RCD1 in vitro. Here, we found the mRNA level of STO (SALT<br/><br>
TOLERANCE) is greatly increased in rcd1-1 mutant after UV-B radiation. However, UV-B-induced<br/><br>
HY5 and CHS expression is partially inhibited in sto mutant. Based on the above results, it is deduced<br/><br>
that the RCD1, working together with STO, is involved in Arabidopsis UV-B signaling.}},
author = {{Jiang, Lei and Wang, Yan and Björn, Lars Olof and Li, Shaoshan}},
issn = {{1474-9092}},
keywords = {{UV-B; ultraviolet radiation; signaling; Arabidopis; rcd1}},
language = {{eng}},
pages = {{838--846}},
publisher = {{Royal Society of Chemistry}},
series = {{Photochemical and Photobiological Sciences}},
title = {{Arabidopsis RADICAL-INDUCED CELL DEATH1 is involved in UV-B signaling.}},
url = {{http://dx.doi.org/10.1039/b901187k}},
doi = {{10.1039/b901187k}},
volume = {{8}},
year = {{2009}},
}