Using microdispensing to manufacture a customized cell dish for microbeam irradiation of single, living cells
(2009) In Nuclear Instruments & Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms 267(7). p.1199-1205- Abstract
- In this paper is described the preparation of patterned cell dishes to be used in studies of low dose irradiation effects on living cells. Using a droplet microdispenser, an 8 mu m thick polypropylene cell substrate, to which cells do not naturally adhere, was coated in a matrix pattern with the cell adhesive mussel protein Cell-Tak. Cells were shown to adhere and grow on the protein-coated spots, but not on the uncoated parts, providing for guided cell growth. Cultivation of isolated cell colonies provides an opportunity to study how low doses of ionizing radiation affect neighbouring un-irradiated cell colonies. (C) 2009 Elsevier B.V. All rights reserved.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1425573
- author
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- irradiation, Microbeam, Patterned cell dish, Microdispensing, Cell substrate, Bystander effect, HepG2 cells
- in
- Nuclear Instruments & Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms
- volume
- 267
- issue
- 7
- pages
- 1199 - 1205
- publisher
- Elsevier
- external identifiers
-
- wos:000266155000028
- scopus:64349101894
- ISSN
- 0168-583X
- DOI
- 10.1016/j.nimb.2009.02.051
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Nuclear Physics (Faculty of Technology) (011013007), Division of Infection Medicine (BMC) (013024020)
- id
- a2347590-91df-4dc2-bd7b-d82feb7ff6ce (old id 1425573)
- date added to LUP
- 2016-04-01 12:55:33
- date last changed
- 2022-01-27 08:18:19
@article{a2347590-91df-4dc2-bd7b-d82feb7ff6ce, abstract = {{In this paper is described the preparation of patterned cell dishes to be used in studies of low dose irradiation effects on living cells. Using a droplet microdispenser, an 8 mu m thick polypropylene cell substrate, to which cells do not naturally adhere, was coated in a matrix pattern with the cell adhesive mussel protein Cell-Tak. Cells were shown to adhere and grow on the protein-coated spots, but not on the uncoated parts, providing for guided cell growth. Cultivation of isolated cell colonies provides an opportunity to study how low doses of ionizing radiation affect neighbouring un-irradiated cell colonies. (C) 2009 Elsevier B.V. All rights reserved.}}, author = {{Nilsson, E.J.C. and Gram, Magnus and Nilsson, J. and Pallon, Jan and Masternak, Anna and Paczesny, J. and Arteaga, Natalia and Elfman, Mikael and Kristiansson, Per and Nilsson, Charlotta and Åkerström, Bo}}, issn = {{0168-583X}}, keywords = {{irradiation; Microbeam; Patterned cell dish; Microdispensing; Cell substrate; Bystander effect; HepG2 cells}}, language = {{eng}}, number = {{7}}, pages = {{1199--1205}}, publisher = {{Elsevier}}, series = {{Nuclear Instruments & Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms}}, title = {{Using microdispensing to manufacture a customized cell dish for microbeam irradiation of single, living cells}}, url = {{http://dx.doi.org/10.1016/j.nimb.2009.02.051}}, doi = {{10.1016/j.nimb.2009.02.051}}, volume = {{267}}, year = {{2009}}, }